NDLI: Filamin Interacts with Epithelial Sodium Channel and Inhibits Its Channel Function

Please wait, while we are loading the content...

Year: 2017 Volume: 292

Year: 2016 Volume: 291

Year: 2015 Volume: 290

Year: 2014 Volume: 289

Year: 2013 Volume: 288

Year: 2013 Volume: 288 Issue: 52

Year: 2013 Volume: 288 Issue: 51

Year: 2013 Volume: 288 Issue: 50

Year: 2013 Volume: 288 Issue: 49

Year: 2013 Volume: 288 Issue: 48

Year: 2013 Volume: 288 Issue: 47

Year: 2013 Volume: 288 Issue: 46

Year: 2013 Volume: 288 Issue: 45

Year: 2013 Volume: 288 Issue: 44

Year: 2013 Volume: 288 Issue: 43

Year: 2013 Volume: 288 Issue: 42

Year: 2013 Volume: 288 Issue: 41

Year: 2013 Volume: 288 Issue: 40

Year: 2013 Volume: 288 Issue: 39

Year: 2013 Volume: 288 Issue: 38

Year: 2013 Volume: 288 Issue: 37

Year: 2013 Volume: 288 Issue: 36

Year: 2013 Volume: 288 Issue: 35

Year: 2013 Volume: 288 Issue: 34

Year: 2013 Volume: 288 Issue: 33

Year: 2013 Volume: 288 Issue: 32

Year: 2013 Volume: 288 Issue: 31

Year: 2013 Volume: 288 Issue: 30

Year: 2013 Volume: 288 Issue: 29

Year: 2013 Volume: 288 Issue: 28

Year: 2013 Volume: 288 Issue: 27

Year: 2013 Volume: 288 Issue: 26

Year: 2013 Volume: 288 Issue: 25

Year: 2013 Volume: 288 Issue: 24

Year: 2013 Volume: 288 Issue: 23

Year: 2013 Volume: 288 Issue: 22

Year: 2013 Volume: 288 Issue: 21

Year: 2013 Volume: 288 Issue: 20

Year: 2013 Volume: 288 Issue: 19

Year: 2013 Volume: 288 Issue: 18

Year: 2013 Volume: 288 Issue: 17

Year: 2013 Volume: 288 Issue: 16

Year: 2013 Volume: 288 Issue: 15

Year: 2013 Volume: 288 Issue: 14

Year: 2013 Volume: 288 Issue: 13

Year: 2013 Volume: 288 Issue: 12

Year: 2013 Volume: 288 Issue: 11

Year: 2013 Volume: 288 Issue: 10

Year: 2013 Volume: 288 Issue: 9

Year: 2013 Volume: 288 Issue: 8

Year: 2013 Volume: 288 Issue: 7

Year: 2013 Volume: 288 Issue: 6

Year: 2013 Volume: 288 Issue: 5

Year: 2013 Volume: 288 Issue: 4

Year: 2013 Volume: 288 Issue: 3

Year: 2013 Volume: 288 Issue: 2

Year: 2013 Volume: 288 Issue: 1

Our NIH years: a confluence of beginnings.

Site-specific phosphorylation of the p65 protein subunit mediates selective gene expression by differential NF-κB and RNA polymerase II promoter recruitment.

Dynamic phosphorylation of tyrosine 665 in pseudopodium-enriched atypical kinase 1 (PEAK1) is essential for the regulation of cell migration and focal adhesion turnover.

TSG-6 protein is crucial for the development of pulmonary hyaluronan deposition, eosinophilia, and airway hyperresponsiveness in a murine model of asthma.

The human W42R Î³D-crystallin mutant structure provides a link between congenital and age-related cataracts.

Sjogren syndrome antigen B (SSB)/La promotes global microRNA expression by binding microRNA precursors through stem-loop recognition.

Tumor necrosis factor-stimulated gene-6 (TSG-6) amplifies hyaluronan synthesis by airway smooth muscle cells.

The intermediate filament vimentin mediates microRNA miR-378 function in cellular self-renewal by regulating the expression of the Sox2 transcription factor.

Mitoferrin-2-dependent Mitochondrial Iron Uptake Sensitizes Human Head and Neck Squamous Carcinoma Cells to Photodynamic Therapy

F-box and Leucine-rich Repeat Protein 5 (FBXL5) Is Required for Maintenance of Cellular and Systemic Iron $Homeostasis^{*}$

Analysis of small molecule ligands targeting the HIV-1 matrix protein-RNA binding site.

Long range effect of mutations on specific conformational changes in the extracellular loop 2 of angiotensin II type 1 receptor.

Identification of STAT2 serine 287 as a novel regulatory phosphorylation site in type I interferon-induced cellular responses.

Integrated Redox Sensor and Effector Functions for Tetrahydrobiopterin- and Glutathionylation-dependent Endothelial Nitric-oxide Synthase $Uncoupling^{*}$

An Extremely Halophilic Proteobacterium Combines a Highly Acidic Proteome with a Low Cytoplasmic Potassium Content

Bile Acids Induce Pancreatic Acinar Cell Injury and Pancreatitis by Activating Calcineurin

AlkB dioxygenase preferentially repairs protonated substrates: specificity against exocyclic adducts and molecular mechanism of action.

Neuregulin mediates F-actin-driven cell migration through inhibition of protein kinase D1 via Rac1 protein.

Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.

Kinetic characterization of nonmuscle myosin IIb at the single molecule level.

Heparin oligosaccharides inhibit chemokine (CXC motif) ligand 12 (CXCL12) cardioprotection by binding orthogonal to the dimerization interface, promoting oligomerization, and competing with the chemokine (CXC motif) receptor 4 (CXCR4) N terminus.

Re-evaluation of the Role of Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP) in Cellular Calcium Signaling

Assembly of subtype 1 influenza neuraminidase is driven by both the transmembrane and head domains.

Dual function of mitochondrial Nm23-H4 protein in phosphotransfer and intermembrane lipid transfer: a cardiolipin-dependent switch.

The polybasic insertion in autotaxin confers specific binding to heparin and cell surface heparan sulfate proteoglycans.

Polycomb Group Protein PHF1 Regulates p53-dependent Cell Growth Arrest and Apoptosis

Characterization of the Staphylococcus aureus rRNA methyltransferase encoded by orfX, the gene containing the staphylococcal chromosome Cassette mec (SCCmec) insertion site.

C1q-induced LRP1B and GPR6 proteins expressed early in Alzheimer disease mouse models, are essential for the C1q-mediated protection against amyloid-ß neurotoxicity.

Myotubularin-related protein 4 (MTMR4) attenuates BMP/Dpp signaling by dephosphorylation of Smad proteins.

Matrix rigidity activates Wnt signaling through down-regulation of Dickkopf-1 protein.

Sorting nexin 1 loss results in D5 dopamine receptor dysfunction in human renal proximal tubule cells and hypertension in mice.

Bim, a proapoptotic protein, up-regulated via transcription factor E2F1-dependent mechanism, functions as a prosurvival molecule in cancer.

Exposure to a cutinase-like serine esterase triggers rapid lysis of multiple mycobacterial species.

Mechanical modulation of ATP-binding affinity of V1-ATPase.

ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase contributes to malignant transformation of intestinal epithelial cells.

The retinol dehydrogenase Rdh10 localizes to lipid droplets during acyl ester biosynthesis.

Matrix metalloproteinase-1-mediated up-regulation of vascular endothelial growth factor-2 in endothelial cells.

Integration and long distance axonal regeneration in the central nervous system from transplanted primitive neural stem cells.

Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway.

Filamin Interacts with Epithelial Sodium Channel and Inhibits Its Channel Function

Acute ß-adrenergic activation triggers nuclear import of histone deacetylase 5 and delays G(q)-induced transcriptional activation.

Interplay between vß3 integrin and nucleolin regulates human endothelial and glioma cell migration.

Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis.

Promiscuous dimerization of the growth hormone secretagogue receptor (GHS-R1a) attenuates ghrelin-mediated signaling.

Anticoagulant activity of a unique sulfated pyranosic (1->3)-ß-L-arabinan through direct interaction with thrombin.

The voltage-dependent anion channel (VDAC) binds tissue-type plasminogen activator and promotes activation of plasminogen on the cell surface.

Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding.

Zebrafish as a model for monocarboxyl transporter 8-deficiency.

PA1 protein, a new competitive decelerator acting at more than one step to impede glucocorticoid receptor-mediated transactivation.

Differential regulation of extracellular tissue inhibitor of metalloproteinases-3 levels by cell membrane-bound and shed low density lipoprotein receptor-related protein 1.

The role of UNC93B1 protein in surface localization of TLR3 receptor and in cell priming to nucleic acid agonists.

Nuclear factor of activated T-cells (NFAT)C2 inhibits Notch receptor signaling in osteoblasts.

Irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6.

Biochemical characterization of individual human glycosylated pro-insulin-like growth factor (IGF)-II and big-IGF-II isoforms associated with cancer.

Oncogenic microRNA-155 down-regulates tumor suppressor CDC73 and promotes oral squamous cell carcinoma cell proliferation: implications for cancer therapeutics.

Neurokinin-1 receptor directly mediates glioma cell migration by up-regulation of matrix metalloproteinase-2 (MMP-2) and membrane type 1-matrix metalloproteinase (MT1-MMP).

Increase in Serum $Ca^{2+/}Mg^{2+}$ Ratio Promotes Proliferation of Prostate Cancer Cells by Activating TRPM7 $Channels^{*}$

Distinct roles of Ser-764 and Lys-773 at the N terminus of von Willebrand factor in complex assembly with coagulation factor VIII.

Selective amplification of classical and atypical prions using modified protein misfolding cyclic amplification.

Murine hyaluronidase 2 deficiency results in extracellular hyaluronan accumulation and severe cardiopulmonary dysfunction.

Cardiolipin-dependent reconstitution of respiratory supercomplexes from purified Saccharomyces cerevisiae complexes III and IV.

Ligand binding to the AMP-activated protein kinase active site mediates protection of the activation loop from dephosphorylation.

Microtubule-associated protein/microtubule affinity-regulating kinase 4 (MARK4) is a negative regulator of the mammalian target of rapamycin complex 1 (mTORC1).

Dynamic Nucleotide-dependent Interactions of Cysteine- and Histidine-rich Domain (CHORD)-containing Hsp90 Cochaperones Chp-1 and Melusin with Cochaperones PP5 and Sgt1

Molecular mechanism of fascin function in filopodial formation.

Mechanistic target of rapamycin complex 1 (mTORC1)-mediated phosphorylation is governed by competition between substrates for interaction with raptor.

A single N-acetylgalactosamine residue at threonine 106 modifies the dynamics and structure of interferon 2a around the glycosylation site.

Designing a soluble near full-length HIV-1 gp41 trimer.

The Mechanism of $Cu^{+}$ Transport ATPases: INTERACTION WITH $CU^{+}$ CHAPERONES AND THE ROLE OF TRANSIENT METAL-BINDING SITES

Identification of microRNA-98 as a therapeutic target inhibiting prostate cancer growth and a biomarker induced by vitamin D.

Phosphorylation and stabilization of Arabidopsis MAP kinase phosphatase 1 in response to UV-B stress.

Year: 2012 Volume: 287

Year: 2011 Volume: 286

Year: 2010 Volume: 285

Year: 2009 Volume: 284

Year: 2008 Volume: 283

Year: 2007 Volume: 282

Year: 2006 Volume: 281

Year: 2005 Volume: 280

Year: 2004 Volume: 279

Year: 2003 Volume: 278

Year: 2002 Volume: 277

Year: 2001 Volume: 276

Year: 2000 Volume: 275

Year: 1999 Volume: 274

Year: 1998 Volume: 273

Year: 1997 Volume: 272

Year: 1996 Volume: 271

Year: 1995 Volume: 270

Year: 1994 Volume: 269

Year: 1993 Volume: 268

Year: 1992 Volume: 267

Year: 1991 Volume: 266

Year: 1990 Volume: 265

Year: 1989 Volume: 264

Year: 1988 Volume: 263

Year: 1987 Volume: 262

Year: 1986 Volume: 261

Year: 1984 Volume: 259

Year: 1982 Volume: 257

Year: 1981 Volume: 256

Year: 1975 Volume: 250

Filamin Interacts with Epithelial Sodium Channel and Inhibits Its Channel Function

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dai, Xiao-qing Li, Qiang Tuli, Jagdeep Li, Shayla S. Chen, Xing-zhen Wang, Qian Liang, Gengqing
Description	Author Affiliation: Wang Q ( Membrane Protein Disease Research Group, Department of Physiology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.)
Abstract	Epithelial sodium channel (ENaC) in the kidneys is critical for Na(+) balance, extracellular volume, and blood pressure. Altered ENaC function is associated with respiratory disorders, pseudohypoaldosteronism type 1, and Liddle syndrome. ENaC is known to interact with components of the cytoskeleton, but the functional roles remain largely unclear. Here, we examined the interaction between ENaC and filamins, important actin filament components. We first discovered by yeast two-hybrid screening that the C termini of ENaC and ß subunits bind filamin A, B, and C, and we then confirmed the binding by in vitro biochemical assays. We demonstrated by co-immunoprecipitation that ENaC, either overexpressed in HEK, HeLa, and melanoma A7 cells or natively expressed in LLC-PK1 and IMCD cells, is in the same complex with native filamin. Furthermore, the biotinylation and co-immunoprecipitation combined assays showed the ENaC-filamin interaction on the cell surface. Using Xenopus oocyte expression and two-electrode voltage clamp electrophysiology, we found that co-expression of an ENaC-binding domain of filamin substantially reduces ENaC channel function. Western blot and immunohistochemistry experiments revealed that the filamin A C terminus (FLNAC) modestly reduces the expression of the ENaC subunit in oocytes and A7 cells. After normalizing the current by plasma membrane expression, we found that FLNAC results in ~50% reduction in the ENaC channel activity. The inhibitory effect of FLNAC was confirmed by lipid bilayer electrophysiology experiments using purified ENaC and FLNAC proteins, which showed that FLNAC substantially reduces ENaC single channel open probability. Taken together, our study demonstrated that filamin reduces ENaC channel function through direct interaction on the cell surface.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	1
Volume Number	288
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2013-01-04
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Contractile Proteins Chemistry Epithelial Sodium Channels Gene Expression Regulation Microfilament Proteins Sodium Metabolism Animals Cell Line, Tumor Cell Membrane Cell Proliferation Cytoskeleton Filamins Glutathione Transferase HEK293 Cells HeLa Cells Homeostasis Kidney Mice Oocytes Protein Interaction Mapping Sodium Channels Swine Two-Hybrid System Techniques Xenopus Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in