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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mooren, Olivia L. Kim, Joanna Cooper, John A. Li, Jinmei |
| Description | Author Affiliation: Mooren OL ( From the Department of Cell Biology and Physiology, Washington University, St. Louis, Missouri 63110.); Kim J ( From the Department of Cell Biology and Physiology, Washington University, St. Louis, Missouri 63110.); Li J ( From the Department of Cell Biology and Physiology, Washington University, St. Louis, Missouri 63110.); Cooper JA ( From the Department of Cell Biology and Physiology, Washington University, St. Louis, Missouri 63110 jacooper@wustl.edu.) |
| Abstract | Endothelial cells (ECs) form a monolayer that serves as a barrier between the blood and the underlying tissue. ECs tightly regulate their cell-cell junctions, controlling the passage of soluble materials and immune cells across the monolayer barrier. We studied the role of N-WASP, a key regulator of Arp2/3 complex and actin assembly, in EC monolayers. We report that N-WASP regulates endothelial monolayer integrity by affecting the organization of cell junctions. Depletion of N-WASP resulted in an increase in transendothelial electrical resistance, a measure of monolayer integrity. N-WASP depletion increased the width of cell-cell junctions and altered the organization of F-actin and VE-cadherin at junctions. N-WASP was not present at cell-cell junctions in monolayers under resting conditions, but it was recruited following treatment with sphingosine-1-phosphate. Taken together, our results reveal a novel role for N-WASP in remodeling EC junctions, which is critical for monolayer integrity and function. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 30 |
| Volume Number | 290 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2015-07-24 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cytoskeleton Metabolism Endothelial Cells Intercellular Junctions Wiskott-Aldrich Syndrome Protein, Neuronal Actin-Related Protein 2-3 Complex Actins Antigens, CD Cadherins Cell Line Lysophospholipids RNA, Small Interfering Skin Cytology Sphingosine Analogs & Derivatives Genetics Research Support, N.I.H., Extramural Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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