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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sierecki, Emma Wu, Selwin K. Parton, Robert G. Alexandrov, Kirill Verma, Suzie Han, Siew Ping Yap, Alpha S. Giles, Nichole Gomez, Guillermo A. Johnston, Wayne Gambin, Yann Michael, Magdalene Guo, Zhong |
| Description | Author Affiliation: Han SP ( From the Division of Molecular Cell Biology, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.) |
| Abstract | Cadherin junctions arise from the integrated action of cell adhesion, signaling, and the cytoskeleton. At the zonula adherens (ZA), a WAVE2-Arp2/3 actin nucleation apparatus is necessary for junctional tension and integrity. But how this is coordinated with cadherin adhesion is not known. We now identify cortactin as a key scaffold for actin regulation at the ZA, which localizes to the ZA through influences from both E-cadherin and N-WASP. Using cell-free protein expression and fluorescent single molecule coincidence assays, we demonstrate that cortactin binds directly to the cadherin cytoplasmic tail. However, its concentration with cadherin at the apical ZA also requires N-WASP. Cortactin is known to bind Arp2/3 directly (Weed, S. A., Karginov, A. V., Schafer, D. A., Weaver, A. M., Kinley, A. W., Cooper, J. A., and Parsons, J. T. (2000) J. Cell Biol. 151, 29-40). We further show that cortactin can directly bind WAVE2, as well as Arp2/3, and both these interactions are necessary for actin assembly at the ZA. We propose that cortactin serves as a platform that integrates regulators of junctional actin assembly at the ZA. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 11 |
| Volume Number | 289 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2014-03-14 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Actin-Related Protein 2 Metabolism Actin-Related Protein 3 Adherens Junctions Cortactin Epithelium Wiskott-Aldrich Syndrome Protein Family Actins Caco-2 Cells Cadherins Cell Adhesion Cell-Free System Cytoskeleton Green Fluorescent Proteins Image Processing, Computer-Assisted Microscopy, Confocal Microscopy, Fluorescence Spectrometry, Fluorescence Wiskott-Aldrich Syndrome Protein, Neuronal Research Support, Non-U.S. Gov't Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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