NDLI: Effect of substitution and planarity of the ligand on DNA /BSA interaction, free radical scavenging and cytotoxicity of diamagnetic Ni( II) complexes: A systematic investigation

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Krishnamoorthy, Paramasivam Cowley, Alan H. Dharmaraj, Nallasamy Sathyadevi, Palanisamy Butorac, Rachel R. Bhuvanesh, Nattamai S. P.
Description	Author Affiliation: Sathyadevi P ( Department of Chemistry, Bharathiar University, Coimbatore, India.)
Abstract	Four new bivalent nickel hydrazone complexes have been synthesised from the reactions of [ NiCl $_{2}(PPh$ $_{3})$ $_{2}$ ] with H $_{2}L$ {L = dianion of the hydrazones derived from the condensation of salicylaldehyde or o-hydroxy acetophenone with p-toluic acid hydrazide ( H $_{2}L$ $^{1}$ ) (1), $(H_{2}L^{2})$ (2) and o-hydroxy acetophenone or o-hydroxy naphthaldehyde with benzhydrazide ( H $_{2}L$ $^{3}$ ) (3) and ( H $_{2}L$ $^{4}$ ) (4)} and formulated as [ Ni(L $^{1})(PPh$ $_{3})$ ] (5), [ Ni(L $^{2})(PPh$ $_{3})$ ] (6), [ Ni(L $^{3})(PPh$ $_{3})$ ] (7) and [Ni(L $^{4})(PPh$ $_{3})]$ (8). Structural characterization of complexes 5–8 were accomplished by using various physico-chemical techniques. In order to study the influence of substitution in the ligand and its planarity on the biological activity of complexes 5–8 containing them, suitable hydrazone ligands 1–4 have been selected in this study. Single crystal diffraction data of complexes 5, 7 and 8 proved the geometry of the complexes to be distorted square planar with a 1:1 ratio between the metal ion and the coordinated hydrazones . To provide more insight on the mode of action of complexes 5–8 under biological conditions, additional experiments involving their interaction with calf thymus DNA (CT DNA ) and bovine serum albumin (BSA) were monitored by UV-visible and fluorescence titrations respectively. Further, the ligands 1–4 and corresponding nickel( II) chelates 5–8 have been tested for their scavenging effect towards OH and $O_{2}^{−}$ radicals. The effect of complexes 5–8 to arrest the growth of HeLa and Hep-2 tumour cell lines has been studied along with the cell viability against the non-cancerous NIH 3T3 cells under in vitro conditions.
ISSN	14779226
Issue Number	38
Volume Number	40
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2011-10-14
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Antineoplastic Agents Pharmacology DNA Chemistry Free Radical Scavengers Nickel Organometallic Compounds Serum Albumin, Bovine Animals Chemical Synthesis Binding Sites Drug Effects Cattle Cell Line, Tumor Cell Proliferation Cell Survival Crystallography, X-Ray Dose-Response Relationship, Drug Drug Screening Assays, Antitumor HeLa Cells Humans Ligands Magnetics Mice Models, Molecular Molecular Structure NIH 3T3 Cells Stereoisomerism Structure-Activity Relationship Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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