NDLI: A versatile water soluble fluorescent probe for ratiometric sensing of $Hg^{2+}$ and bovine serum albumin

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wen, Jinghan Yin, Yuxin Geng, Zhirong Wang, Zhilin
Description	Author Affiliation: Wen J ( State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, PR China.)
Abstract	A novel tetraazamacrocycle fluorescent sensor ( 6-(1-(dimethylamino)-5-naphthalene sulfonyl)-3,6,9,15-tetraazabicyclo[9.3.1] pentadeca-1(15),11,13-triene , 1) has been designed and prepared, which can be utilized for selective and ratiometric sensing of $Hg^{2+}$ and bovine serum albumin (BSA) with two different responsive modes in aqueous solution at physiological pH (50 mM Tris-HCl, pH 7.6). Above 0.5 ppb $Hg^{2+}$ can be discerned by coordination with 1 and the emission color changes enable 1 to be applied to a fast $Hg^{2+}$ test paper assay. Sensor 1 has also been demonstrated to be easily cell-penetrable and applicable for $Hg^{2+}$ imaging in living cells. Imaging of BSA in the gel using SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) stained in the medium containing 1 verified that the binding of 1 and BSA was successful in the presence of nonprotein substances. The linear range of 1 towards BSA utilizing ratiometric fluorescent calibration via noncovalent interaction in solution is 0–100 μg $mL^{−1}$ with a detection limit of 1 μg $mL^{−1},$ and has been successfully employed to determine the albumin concentration in blood serum by means of ratiometric fluorescent measurements for the first time. Finally, sensor 1 behaves as a fluorescent molecular switch composed of triple logic gates upon chemical inputs of $Hg^{2+}$ and BSA, which potentially provides intelligent diagnostics for $Hg^{2+}$ contaminated serum on the nanoscale.
ISSN	14779226
Issue Number	38
Volume Number	40
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2011-10-14
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Fluorescent Dyes Chemistry Mercury Analysis Serum Albumin, Bovine Animals Cattle Chemical Synthesis HeLa Cells Humans Hydrogen-Ion Concentration Microscopy, Fluorescence Molecular Structure Particle Size Quantum Theory Solubility Stereoisomerism Surface Properties Water Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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