NDLI: $[Ru(bpy)_{2}dppz-idzo]^{2+}:$ a colorimetric molecular “light switch” and powerful stabilizer for G-quadruplex DNA

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yao, Jun-liang Gao, Xing Sun, Wenliang Shi, Shuo Yao, Tian-ming
Description	Author Affiliation: Yao JL ( Department of Chemistry, Tongji University, Shanghai, China.)
Abstract	A new ruthenium complex, $[Ru(bpy)_{2}dppz-idzo]^{2+}$ ( bpy = 2,2′-bipyridine , dppz-idzo = dipyrido-[3,2-a:2′,3′-c] phenazine - imidazolone ), was synthesized and characterized. The luminescent titrations showed that the Ru-complex exhibited an outstanding “light switch” effect with an emission enhancement factor of about 300 in the presence of G-quadruplex DNA in a $K^{+}$ solution. This remarkable “light switch” behavior can even be observed by the naked eye under irradiation with UV light. To get an insight into the “light switch” mechanism, quantum-chemical calculations were performed based on the DFT/TDDFT/PCM method at the B3LYP/6-31G* level. Furthermore, the CD titrations and thermal melting experiments indicated that $[Ru(bpy)_{2}dppz-idzo]^{2+}$ could not only induce the formation of an antiparallel G-quadruplex structure in the absence of monocations, but also has the ability to stabilize the G-quadruplex architecture, implying potential applications in anticancer therapeutics. Both the “light switch” effect and the structure stabilization ability of $[Ru(bpy)_{2}dppz-idzo]^{2+}$ were found to be superior to the well-known DNA molecular “light switch” $[Ru(bpy)_{2}dppz]^{2+}.$ Finally, a “sandwich-like” binding model was proposed on the basis of molecular docking simulations.
ISSN	14779226
Issue Number	16
Volume Number	42
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2013-04-28
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	G-Quadruplexes Organometallic Compounds Chemistry Phenazines Ultraviolet Rays Binding Sites Circular Dichroism Colorimetry Ions Molecular Conformation Molecular Docking Simulation Chemical Synthesis Potassium Quantum Theory Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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