NDLI: Development of Fluorescent Glucose Bioprobes and Their Application on Real-Time and Quantitative Monitoring of Glucose Uptake in Living Cells

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Development of Fluorescent Glucose Bioprobes and Their Application on Real-Time and Quantitative Monitoring of Glucose Uptake in Living Cells

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Park, Seung Bum Lee, Jae Jeong Lee, Hyang Yeon Park, Jongmin
Description	Author Affiliation: Lee HY ( Department of Chemistry, Seoul National University, Seoul 151-747, Korea.)
Abstract	We developed a novel fluorescent glucose bioprobe, GB2-Cy3, for the real-time and quantitative monitoring of glucose uptake in living cells. We synthesized a series of fluorescent glucose analogues by adding Cy3 fluorophores to the α-anomeric position of D-glucose through various linkers. Systematic and quantitative analysis of these Cy3-labeled glucose analogues revealed that GB2-Cy3 was the ideal fluorescent glucose bioprobe. The cellular uptake of this probe competed with the cellular uptake of D-glucose in the media and was mediated by a glucose-specific transport system, and not by passive diffusion. Flow cytometry and fluorescence microscopy analyses revealed that GB2-Cy3 is ten times more sensitive than 2-NBDG, a leading fluorescent glucose bioprobe. GB2-Cy3 can also be utilized for the quantitative flow cytometry monitoring of glucose uptake in metabolically active C2C12 myocytes under various treatment conditions. As opposed to a glucose uptake assay performed by using radioisotope-labeled deoxy-D-glucose and a scintillation counter, GB2-Cy3 allows the real-time monitoring of glucose uptake in living cells under various experimental conditions by using fluorescence microscopy or confocal laser scanning microscopy (CLSM). Therefore, we believe that GB2-Cy3 can be utilized in high-content screening (HCS) for the discovery of novel therapeutic agents and for making significant advances in biomedical studies and diagnosis of various diseases, especially metabolic diseases.
ISSN	09476539
e-ISSN	15213765
Journal	Chemistry - A European Journal
Issue Number	1
Volume Number	17
Language	English
Publisher	Wiley-VCH;ChemPubSoc Europe
Publisher Date	2011-01-03
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	4-Chloro-7-nitrobenzofurazan Analogs & Derivatives Deoxyglucose Fluorescent Dyes Chemistry Glucose Metabolism Pharmacology Animals Biological Transport Biosensing Techniques Mice NIH 3T3 Cells Time Factors Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Catalysis

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