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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Seng, Jo Ann Ellis, Shane R. Mitchell, Todd W. Hughes, Jessica R. Maccarone, Alan T. Truscott, Roger J. W. Blanksby, Stephen J. |
| Description | Author Affiliation: Seng JA ( ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, School of Chemistry, University of Wollongong, NSW 2522, Australia.); Ellis SR ( ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, School of Chemistry, University of Wollongong, NSW 2522, Australia.); Hughes JR ( School of Medicine, University of Wollongong, NSW 2522, Australia.); Maccarone AT ( ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, School of Chemistry, University of Wollongong, NSW 2522, Australia.); Truscott RJ ( Illawarra Health and Medical Research Institute, University of Wollongong, NSW 2522, Australia.); Blanksby SJ ( ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, School of Chemistry, University of Wollongong, NSW 2522, Australia. Electronic address: stephen.blanksby@qut.edu.au.); Mitchell TW ( School of Medicine, University of Wollongong, NSW 2522, Australia) |
| Abstract | The lipidome of the human lens is unique in that cholesterol and dihydrosphingomyelin are the dominant classes. Moreover, the lens lipidome is not static with dramatic changes in several sphingolipid classes associated with both aging and cataract. Accordingly, there is a clear need to expand knowledge of the molecular species that constitute the human lens sphingolipidome. In this study, human lens lipids have been extracted and separated by thin-layer chromatography (TLC). Direct analysis of the TLC plates by desorption electrospray ionisation–mass spectrometry (DESI–MS) allowed the detection over 30 species from 11 classes of sphingolipids. Significantly, novel classes of lens lipids including sulfatides, dihydrosulfatides, lactosylceramide sulfates and dihydrolactosylceramide sulfates were identified. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 9 |
| Volume Number | 1841 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-09-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Cholesterol Isolation & Purification Lens, Crystalline Chemistry Sphingolipids Chromatography, Thin Layer Spectrometry, Mass, Electrospray Ionization Classification Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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