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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mukhtarova, Lucia S. Gorina, Svetlana S. Grechkin, Alexander N. Toporkova, Yana Y. Chechetkin, Ivan R. Gogolev, Yuri V. Khairutdinov, Bulat I. |
| Description | Author Affiliation: Gorina SS ( Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia.); Toporkova YY ( Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia.); Mukhtarova LS ( Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia.); Chechetkin IR ( Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia.); Khairutdinov BI ( Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia.); Gogolev YV ( Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia.); Grechkin AN ( Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, 420111 Kazan, Russia. Electronic address: grechkin@mail.knc.ru.) |
| Abstract | Enzymes of the CYP74 family, including the divinyl ether synthase (DES), play important roles in plant cell signalling and defence. The potent DES activities have been detected before in the leaves of the meadow buttercup (Ranunculus acris L.) and few other Ranunculaceae species. The nature of these DESs and their genes remained unrevealed. The PCR with degenerate primers enabled to detect the transcript of unknown P450 gene assigned as CYP74Q1. Besides, two more CYP74Q1 isoforms with minimal sequence variations have been found. The full length recombinant CYP74Q1 protein was expressed in Escherichia coli. The preferred substrates of this enzyme are the 13-hydroperoxides of α-linolenic and linoleic acids, which are converted to the divinyl ether oxylipins (ω5Z)-etherolenic acid, (9Z,11E)-12-[(1′Z,3′Z)-hexadienyloxy]-9,11-dodecadienoic acid, and (ω5Z)-etheroleic acid, (9Z,11E)-12-[(1′Z)-hexenyloxy]-9,11-dodecadienoic acid, respectively, as revealed by the data of mass spectrometry, NMR and UV spectroscopy. Thus, CYP74Q1 protein was identified as the R. acris DES (RaDES), a novel DES type and the opening member of new CYP74Q subfamily. |
| ISSN | 00063002 |
| Journal | Biochimica et Biophysica Acta (BBA) - Reviews on Cancer |
| Issue Number | 9 |
| Volume Number | 1841 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-09-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Cytochrome P-450 Enzyme System Metabolism Phylogeny Chemistry Plant Proteins Ranunculus Amino Acid Sequence Cloning, Molecular Classification Genetics DNA Primers Escherichia Coli Gene Expression Isoenzymes Kinetics Linoleic Acids Molecular Sequence Data Oxylipins Enzymology Polymerase Chain Reaction Recombinant Proteins Sequence Alignment Sequence Homology, Amino Acid Substrate Specificity Alpha-Linolenic Acid Research Support, Non-U.S. Gov't Biochemistry |
| Content Type | Text |
| Resource Type | Article |
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