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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tan, Tianwei Lv, Yongqin Lin, Zhixing Svec, Frantisek |
| Description | Country affiliation: United States Author Affiliation: Lv Y ( Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China) |
| Abstract | Monolithic 50 µm thin poly(4-methylstyrene-co-chloromethylstyrene-co-divinylbenzene) layers attached to 6.0 cm × 3.3 cm glass plates have been prepared, using a thermally initiated polymerization process. These layers had a well-defined porous structure with a globular morphology demonstrated with SEM images and exhibited superhydrophobic properties characterized with a water contact angle of 157°. They were then used for thin-layer chromatography of peptides and proteins fluorescently labeled with fluorescamine. The spots of individual separated compounds were visualized using UV light, and their identities were confirmed with a matrix-assisted laser desorption/ionization time of flight mass spectrometry. The presence of chloromethylstyrene units in the polymer enabled hypercrosslinking via a Friedel-Crafts alkylation reaction, and led to monoliths with much larger surface areas, which were suitable for separations of small dye molecules. |
| ISSN | 00219673 |
| Volume Number | 1316 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-11-05 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chromatography, Thin Layer Instrumentation Methods Polystyrenes Chemistry Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Coloring Agents Isolation & Purification Peptides Polymerization Chemical Synthesis Proteins Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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