NDLI: Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion.

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Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion.

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Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Klinger, H. P. Shin, S. I.
Abstract	Mouse A9 cells, deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8), were fused with normal chick erythrocytes and selected in hypoxanthine-aminopterin-thymidine medium for cells with hypoxanthine phosphoribosyltransferase activity. Recovered hybrid cells produced the chick hypoxanthine phosphoribosyltransferase exclusively, as demonstrated by electrophoretic mobility and immunoprecipitation tests, even though no chick chromosomes or chick cell-surface antigens could be identified in the hybrids. Surprisingly, the expression of the chick hypoxanthine phosphoribosyl-transferase activity in the mouse/chick hybrids required the presence of aminopterin in the growth medium; in its absence, enzyme synthesis decreased markedly. Because of the rapid and reversible modulation of hypoxanthine phosphoribosyltransferase activity, the hybrid cells could proliferate equally well in media containing hypoxanthine-aminopterin-thymidine or 8-azaguanine. Cellular selection was definitely ruled out as a possible cause. These results confirm previous reports that specific genetic information can be selectively transferred from one cell to another of a distant species. Furthermore, they demonstrate that an avian gene, whose activity is normally expressed constitutively, can become facultative when integrated into a mammalian cell. This seems to be the first instance where heterologous gene activity has been shown to be reversibly modulated in hybrid cells.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	4
Volume Number	71
Language	English
Publisher	National Academy of Sciences
Publisher Date	1974-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Genes Hybrid Cells Enzymology Pentosyltransferases Biosynthesis Aminopterin Animals Azaguanine Cell Fusion Chick Embryo Chromatography, Paper Clone Cells Culture Media Electrophoresis Enzyme Induction Enzyme Repression Erythrocytes Hypoxanthines Mice Precipitin Tests Species Specificity Thymidine Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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