NDLI: Mitogenic activity of blood components. I. Thrombin and prothrombin.

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Mitogenic activity of blood components. I. Thrombin and prothrombin.

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Mitogenic activity of blood components. I. Thrombin and prothrombin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, L. B. Buchanan, J. M.
Abstract	When added to a culture medium of resting confluent chick embryo fibroblasts in the absence of serum, thrombin (EC 3.4.21.5) is able to stimulate DNA synthesis 12 hr later and to cause a substantial increase in cell number over a period of 4 days. As compared to thrombin, prothrombin exhibits low mitogenic activity. However, in the presence of purified Factor Xa (EC 3.4.21.6) and Factor V, prothrombin is converted to thrombin by 'thromboplastin activity' supplied by the fibroblasts. Prothrombin, either purified or as a constituent of plasma or serum, may thus be considered to be a reservoir of mitogenic activity in tissue culture unless antithrombin is present in the culture medium in amounts sufficient to neutralize the thrombin formed. By use of a specific inhibitor of proteases, and by separation of prothrombin by absorption on BaSO4, we estimate that the potential mitogenic activity of prothrombin is approximately 30-50% of the total activity that can be obtained by treatment of fibrinogen-free plasma with thromboplastin. In addition to its mitogenic activity, thrombin can also stimulate the migration of cells. These experiments with thrombin illustrate that well-characterized proteases of blood can act as potent mitogens and suggest that they may play a role in the process of wound healing.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	72
Language	English
Publisher	National Academy of Sciences
Publisher Date	1975-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Mitogens Prothrombin Pharmacology Thrombin Animals Chick Embryo DNA Biosynthesis Fibroblasts Metabolism Growth Substances Mitosis Drug Effects Wound Healing Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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