NDLI: Nature of conformational changes in poly[d(A-T)-d(A-T)] in the premelting region.

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Nature of conformational changes in poly[d(A-T)-d(A-T)] in the premelting region.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Brahms, J. Brahms, S. Van Holde, K. E.
Abstract	The conformation of the synthetic DNA, poly-[d(A-T)-d(A-T)], has been investigated both in the solid state and in dilute aqueous solutions at different temperatures below its melting point. The change of the circular dichroism (CD) spectra of poly[d(A-T)-d(A-T)] solutions with decreasing temperatures from just below the melting point to 0 degrees involves a specific decrease of the intensity of the 262 nm CD band. This conformational change has been assigned to a gradual and partial transition from the B to C form, on the basis of the following results: (i) By the use of infrared dichroism measurements on oriented films we have defined humidity and salt conditions under which B and C forms of poly[d(A-T)-d(A-T](Li+) are stable. In addition, we find that ammonium salts induce the C form of poly[d(A-T)-d(A-T)] even at high relative humidity. (ii) CD studies of the films of the lithium salt of poly[d(A-T)-d(A-T)] under the same conditions have given CD spectra corresponding to the B and C forms of this polynucleotide. In addition, the CD spectrum of the ammonium salt of poly[d(A-T)-d(A-T)] in solution approaches that of the C form in films. (iii) The conformational change of poly[d(A-T)-d(A-T)] as a function of temperature can be entirely explained on the basis of changes in the double-stranded base-paired structure. Our data rule out hydrogen bond breaking and unstacking or 'breathing' as an explanation of the premelting changes. Curves of the continuous variation of CD(epsilon at 262 nm) as a function of temperature (from 0 degrees to the melting zone) show similar slopes in the presence of different agents stabilizing the double-stranded structure, such as Mg++, or at different salt concentration (KCl), indicating that the nature of the process is independent of ionic strength. Some specific effects were observed in the influence of certain neutral salts; ammonium induces the C form whereas magnesium favors the B form. CD data give direct evidence that a DNA like poly[d(A-T)-d(A-T)] need not change conformation upon transition from a dilute aqueous solution to a highly hydrated (film/gel) solid state. The change of conformation begins only at a defined partial dehydration.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	73
Language	English
Publisher	National Academy of Sciences
Publisher Date	1976-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Nucleic Acid Conformation Nucleic Acid Denaturation Poly DA-dT Polydeoxyribonucleotides Circular Dichroism Spectrophotometry, Infrared Temperature Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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