NDLI: Cloning of an origin of DNA replication of Xenopus laevis.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1980 Volume: 77 Issue: 12

Year: 1980 Volume: 77 Issue: 11

Year: 1980 Volume: 77 Issue: 10

Year: 1980 Volume: 77 Issue: 9

Unique long-acting antiglucocorticoid in whole and broken cell systems.

Release of immunoreactive somatostatin from hypothalamic cells in culture: inhibition by gamma-aminobutyric acid.

Amino-terminal amino acid sequence of human leukocyte interferon.

Mechanism of translocation: relative arrangement of tRNA and mRNA on the ribosome.

Genetic structure of avian myeloblastosis virus, released from transformed myeloblasts as a defective virus particle.

Solubilization of hemoglobin S by other hemoglobins.

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Expression of the human fibroblast interferon gene in Escherichia coli.

Localization of androgen-binding protein in proliferating Sertoli cells in culture.

Le interferon mRNA from human fibroblasts.

Action of granulocyte-macrophage colony-stimulating factors: studies using a human leukemia cell line.

Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.

Establishment of the interferon-mediated antiviral state: role of fatty acid cyclooxygenase.

Specific inhibition of renin by an angiotensinogen analog: studies in sodium depletion and renin-dependent hypertension.

Brain regulation of gastric secretion: influence of neuropeptides.

Nucleotide sequence of influenza virus RNA segment 8 indicates that coding regions for NS1 and NS2 proteins overlap.

Nucleosomal packaging of the thymidine kinase gene of herpes simplex virus transferred into mouse cells: an actively expressed single-copy gene.

Crystallization of the middle part of the mitochondrial electron transfer chain: cytochrome bc1-cytochrome c complex.

Reconstitution of the hepatic asialoglycoprotein receptor with phospholipid vesicles.

Construction of a composite tRNA gene by anticodon loop transplant.

Cloning and analysis of a cDNA coding for bovine prothrombin.

Origin and functional properties of the major gene product of the Snyder-Theilen strain of feline sarcoma virus.

Acyclovir inhibition of Epstein-Barr virus replication.

Novel bacteriophage lambda cloning vector.

Cellular sequences are present in the presumptive avian myeloblastosis virus genome.

In vitro DNA replication of recombinant plasmid DNAs containing the origin of progeny replicative form DNA synthesis of phage phi X174.

Autoradiographic detection of animal cell membrane mutants altered in phosphatidylcholine synthesis.

Antibodies specific for the carboxy- and amino-terminal regions of simian virus 40 large tumor antigen.

Processing of the precursor to corticotropin and beta-lipotropin in humans.

Origin of DNA replication of bacteriophage f1 as the signal for termination.

Nucleotide sequence analysis of the transforming region and large terminal redundancies of Moloney murine sarcoma virus.

Electron paramagnetic resonance spectroscopy of Cu2+ in hen egg-white lysozyme.

Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.

Cloning of an origin of DNA replication of Xenopus laevis.

Monoclonal antibodies against the major glycoprotein (gp350/220) of Epstein-Barr virus neutralize infectivity.

Messenger RNA prevalence in sea urchin embryos measured with cloned cDNAs.

Regulation of stromal cell collagenase production in adult rabbit cornea: in vitro stimulation and inhibition by epithelial cell products.

Putative actin genes in the macronucleus of Oxytricha fallax.

Activation of cellular genes by avian RNA tumor viruses.

Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule.

Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B.

Replication and expression of thymidine kinase and human globin genes microinjected into mouse fibroblasts.

Effect of diethyldithiocarbamate rescue on tumor response to cis-platinum in a rat model.

Transfer of Epstein-Barr virus receptors to receptor-negative cells permits virus penetration and antigen expression.

Low density lipoprotein receptor activity in human monocyte-derived macrophages and its relation to atheromatous lesions.

Generation of an electrochemical proton gradient in Streptococcus cremoris by lactate efflux.

Expression of cloned hepatitis B virus DNA in human cell cultures.

Peptide inhibitor of morphine- and beta-endorphin-induced analgesia.

Morphine and opioid peptides reduce paraventricular neuronal activity: studies on the rat hypothalamic slice preparation.

In vitro autoradiography of opiate receptors in rat brain suggests loci of 'opiatergic' pathways.

Localization of cyclic GMP-dependent protein kinase and substrate in mammalian cerebellum.

Adenosine receptors in brain membranes: binding of N6-cyclohexyl[3H]adenosine and 1,3-diethyl-8-[3H]phenylxanthine.

Similar luteinizing hormone-releasing hormone binding sites in rat anterior pituitary and ovary.

Overlapping genes at the cheA locus of Escherichia coli.

Syngeneic tumor cells can induce alloreactive T killer cells: a biological role for transplantation antigens.

Role of arachidonic acid in stimulation of hexose transport by human polymorphonuclear leukocytes.

Origins of metabolic diversity: substitution of homologous sequences into genes for enzymes with different catalytic activities.

Interaction between I region loci influences the expression of a cell surface Ia antigen.

Successive switching of antibody isotypes expressed within the lines of a B-cell clone.

Lasting damage to bacterial ribosomes by reversibly bound virginiamycin M.

Light-induced calcium release by intact retinal rods.

Mitochondrial carbonic anhydrase.

Mechanism of negative cooperativity in glyceraldehyde-3-phosphate dehydrogenase deduced from ligand competition experiments.

Scanning calorimetric evidence for a third phase transition in phosphatidylcholine bilayers.

Human plasma fibrinogen heterogeneity: evidence for an extended carboxyl-terminal sequence in a normal gamma chain variant (gamma').

Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands.

Cell cycle regulation of dihydrofolate reductase mRNA metabolism in mouse fibroblasts.

Photochemically induced dynamic nuclear polarization investigation of complex formation of the NH2-terminal DNA-binding domain of lac repressor with poly[d(AT)].

Enzymatic reduction of alloxan by thioredoxin and NADPH-thioredoxin reductase.

Communication between catalytic subunits in hybrid aspartate transcarbamoylase molecules: effect of ligand binding to active chains on the conformation of unliganded, inactive chains.

Increase of NAD(P)H:quinone reductase by dietary antioxidants: possible role in protection against carcinogenesis and toxicity.

Pyrroline-5-carboxylate synthase activity in mammalian cells.

Ligand-linked phase changes in a biological system: applications to sickle cell hemoglobin.

The energy relay: a proofreading scheme based on dynamic cooperativity and lacking all characteristic symptoms of kinetic proofreading in DNA replication and protein synthesis.

Chloroplast phosphoproteins: regulation of excitation energy transfer by phosphorylation of thylakoid membrane polypeptides.

Carbohydrate requirement for expression and stability of acetylcholine receptor on the surface of embryonic muscle cells in culture.

Prolonged survival of bovine adrenal chromaffin cells in rat cerebral ventricles.

A colchicine-sensitive uptake system in Morris hepatomas.

Helper virus is not required for in vitro erythroid transformation of hematopoietic cells by Friend virus.

Local anesthetics inhibit induction of ornithine decarboxylase by the tumor promoter 12-O-tetradecanoylphorbol 13-acetate.

Maintenance of an aminopeptidase allele frequency cline by natural selection.

Protection of cultured renal tubular epithelial cells from anoxic cell swelling and cell death.

Molecular abnormality of phosphoglycerate kinase-Uppsala associated with chronic nonspherocytic hemolytic anemia.

Prolonged derangements of canine myocardial purine metabolism after a brief coronary artery occlusion not associated with anatomic evidence of necrosis.

Albumin Naskapi variant in North American Indians and Eti Turks.

Determination of deoxyhemoglobin S polymer in sickle erythrocytes upon deoxygenation.

Analgesic activity of the naturally occurring heptapeptide [Met]enkephalin-Arg6-Phe7.

The differential effects of ionizing radiation on the circadian oscillator and other functions in the eye of Aplysia.

Stereoacuity of human infants.

Clonal analysis of proliferation and differentiation of paired daughter cells: action of granulocyte-macrophage colony-stimulating factor on granulocyte-macrophage precursors.

Rat dendritic cells function as accessory cells and control the production of a soluble factor required for mitogenic responses of T lymphocytes.

Selective killing of normal or neoplastic B cells by antibodies coupled to the A chain of ricin.

Development and characterization of allospecific long-term human cytolytic T-cell lines.

Anti-Thy 1.2 monoclonal antibody linked to ricin is a potent cell-type-specific toxin.

Differential amplification of specific areas of phage T4 genome as revealed by hybridization to cloned genetic segments.

Novel histone H2A-like protein of escherichia coli.

Monoclonal antibodies to human estrogen receptor.

Comparison of the structures of free and ribosome-bound tRNAPhe by using slow tritium exchange.

Chinese hamster lung cells synthesize and confine to the cellular domain a collagen composed solely of B chains.

Reversible translocation of cytoplasmic actin into the nucleus caused by dimethyl sulfoxide.

Selective effects of phorbol 12-myristate 13-acetate on myofibrils and 10-nm filaments.

Preferential inclusion of extrachromosomal genetic elements in yeast meiotic spores.

Genetic manipulation by means of microcell-mediated transfer of normal human chromosomes into recipient mouse cells.

Cleavage of fibrinogen by the human neutrophil neutral peptide-generating protease.

Wide distribution of immunoreactive renin in nerve cells of human brain.

Regulation of beta-galactoside phosphate accumulation in Streptococcus pyogenes by an expulsion mechanism.

Brain clathrin: immunofluorescent patterns in cultured cells and tissues.

Year: 1980 Volume: 77 Issue: 8

Year: 1980 Volume: 77 Issue: 7

Year: 1980 Volume: 77 Issue: 6

Year: 1980 Volume: 77 Issue: 5

Year: 1980 Volume: 77 Issue: 4

Year: 1980 Volume: 77 Issue: 3

Year: 1980 Volume: 77 Issue: 2

Year: 1980 Volume: 77 Issue: 1

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Cloning of an origin of DNA replication of Xenopus laevis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Watanabe, S. Taylor, J. H.
Abstract	DNA fragments of Xenopus laevis, the African frog, were cloned in the EcoRI site of the Escherichia coli plasmid pACYC189 and tested for ability to initiate and complete replication of the recombinant plasmid when injected into unfertilized eggs of X. laevis. After measurement of the [3H]-thymidine incorporation per egg for a number of recombinant plasmids, pSW14 and pSW9, which respectively contain a small segment (550 base pairs) and several kilobases of frog DNA, were selected for more extensive analysis. In spite of the small size of the segment in pSW14, it incorporates in 2 hr at least 3 times as much labeled thymidine as either pSW9 or the vector alone. The DNA synthesis in pSW14 was shown to be replication rather than repair synthesis, based on a buoyant density shift of the product when iododeoxyuridine was used for labeling. To determine the number of replications of pSW14, a novel method was employed. Because pSW14 is a head-to-head dimer of the vector with the Xenopus fragment inserted at an EcoRI site, the plasmid has three methylatable sites--two bracketing the Xenopus fragment and one opposite the fragment. By cotransformation of E. coli with pSW14 and pBR322 containing the EcoRI methylase gene, supercoiled pSW14 was methylated and injected into eggs with [3H]thymidine. Disappearance of modified EcoRI sites by semiconservative replication was followed by measuring the sensitivity to EcoRI endonuclease over time. The results showed that about 50% of the labeled, supercoiled DNA recovered from eggs after 4 hr was sensitive to EcoRI digestion, which indicates that most of the DNA that incorporated [3H]thymidine had replicated twice during the 4 hr in the unfertilized eggs of X. laevis. We conclude that pSW14 has a functional origin in the Xenopus DNA segment.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	77
Language	English
Publisher	National Academy of Sciences
Publisher Date	1980-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA Replication DNA, Recombinant Metabolism Gene Expression Regulation Xenopus Genetics Animals Cloning, Molecular DNA, Superhelical Biosynthesis Escherichia Coli Genetic Vectors Microinjections Ovum Plasmids Replicon Transformation, Genetic Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in