NDLI: Acoustic microscopy of living cells.

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Isolation of lymphocyte membrane complement receptor type two (the C3d receptor) and preparation of receptor-specific antibody.

Lens transglutaminase and cataract formation.

Formation of 11-trans slow reacting substances.

Increased nuclear conjugated polyamines and transglutaminase during liver regeneration.

Construction of recombinant plasmids containing Xenopus immunoglobulin heavy chain DNA sequences.

Synthesis of immunoglobulin mu chain gene products precedes synthesis of light chains during B-lymphocyte development.

Epstein-Barr virus RNA VII: size and direction of transcription of virus-specified cytoplasmic RNAs in a transformed cell line.

Autoradiographic localization of benzodiazepine receptors in immunocytochemically identified gamma-aminobutyrergic synapses.

gamma-Aminobutyric acid and benzodiazepine receptors: copurification and characterization.

Gastrointestinal secretory, motor, circulatory, and metabolic effects of prosomatostatin.

Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA.

Selective inhibition of Escherichia coli protein synthesis and growth by nonionic oligonucleotides complementary to the 3' end of 16S rRNA.

Site-specific cleavage by T1 RNase of U-1 RNA in u-1 ribonucleoprotein particles.

Partial purification and characterization of human gamma (immune) interferon.

Human placentas contain a specific inhibitor of RNA-directed DNA polymerase.

Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.

Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.

Does OKT3 monoclonal antibody react with an antigen-recognition structure on human T cells?

Production of hybridomas secreting monoclonal antibodies to the human leukocyte interferons.

Reconstitution of Ir genes, Ia antigens, and mixed lymphocyte reaction determinants by gene complementation.

Detection of a recombinant murine leukemia virus-related glycoprotein on virus-negative thymoma cells.

Developmental regulation of myelin basic protein in dispersed cultures.

Nucleotide sequences at the termini of phi 29 DNA.

Obligatory separation of hormone binding and biological response curves in systems dependent upon secondary mediators of hormone action.

Nucleotide sequence of a ribosomal RNA gene intron from slime mold Physarum polycephalum.

Mapping a eukaryotic promoter: a DNA sequence required for in vivo expression of adenovirus pre-early functions.

Unspliced functional late 19S mRNAs containing intervening sequences are produced by a late leader mutant of simian virus 40.

Saturation and suppression of hepatic lipoprotein receptors: a mechanism for the hypercholesterolemia of cholesterol-fed rabbits.

Biological activity of the spleen focus-forming virus is encoded by a molecularly cloned subgenomic fragment of spleen focus-forming virus DNA.

DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus.

Contacts between DNA gyrase and its binding site on DNA: features of symmetry and asymmetry revealed by protection from nucleases.

Conservation of the primosome in successive stages of phi X174 DNA replication.

Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.

Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.

Induction of the Z conformation in poly(dG-dC).poly(dG-dC) by binding of N-2-acetylaminofluorene to guanine residues.

Diphosphatidylglycerol is required for optimal activity of beef heart cytochrome c oxidase.

Characterization of a cDNA clone coding for the beta chain of bovine fibrinogen.

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Relationship between the Na+/H+ antiporter and Na+/substrate symport in Bacillus alcalophilus.

Changes during fasting in the activity of a specific lysosomal proteinase, fructose-1,6-bisphosphatase converting enzyme.

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Presence of a highly repetitive and widely dispersed DNA sequence in the human genome.

Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.

Molecular cloning of avian myelocytomatosis virus (MC29) transforming sequences.

Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase.

Role of DNA gyrase subunits in synthesis of bacteriophage phi X174 viral DNA.

Evidence that the Abelson virus protein functions in vivo as a protein kinase that phosphorylates tyrosine.

Immunological evidence for the transmembrane nature of the rat liver receptor for asialoglycoproteins.

Tissue-specific exposure of chromatin structure at the 5' terminus of the rat preproinsulin II gene.

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Iodoazidobenzylpindolol, a photoaffinity probe for the beta-adrenergic receptor.

Instability and reiteration of DNA sequences within the vaccinia virus genome.

Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).

Structural and functional domains of the Rous sarcoma virus transforming protein (pp60src).

Spectroscopic characterization of compound C and related derivatives of cytochrome oxidase.

Instability of integrated viral DNA in mouse cells transformed by simian virus 40.

Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane.

Regulatory interactions between mitochondrial genes: interactions between two mosaic genes.

Expression of IgD may use both DNA rearrangement and RNA splicing mechanisms.

Characterization and distribution of nucleic acid sequences of a novel type C retrovirus isolated from neoplastic human T lymphocytes.

Independent recombination between avian leukosis virus terminal sequences and host DNA in virus-induced proliferative disease.

Evidence for three classes of avian sarcoma viruses: comparison of the transformation-specific proteins of PRCII, Y73, and Fujinami viruses.

Locations of adenovirus genes required for the replication of adenovirus-associated virus.

Mechanism of the actomyosin ATPase: effect of actin on the ATP hydrolysis step.

Activation of plasminogen to plasmin by a protease associated with the outer membrane of Escherichia coli.

15N NMR studies of nitrogen metabolism in intact mycelia of Neurospora crassa.

Immune labeling of the D and E regions of human fibrinogen by electron microscopy.

Depurination causes mutations in SOS-induced cells.

Reduced tumor growth after low-dose irradiation or immunization against blastic suppressor T cells.

Digestibility of sorghum proteins.

Resonance Raman spectroscopy of specifically [epsilon-15N]lysine-labeled bacteriorhodopsin.

Recovery of protein synthesis after heat shock: prior heat treatment affects the ability of cells to translate mRNA.

Biochemical evidence for membrane disintegration in human cataracts.

Systematic punctuation of eukaryotic DNA by A+T-rich sequences.

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Specific reaction rate of acetylcholine receptor-controlled ion translocation: a comparison of measurements with membrane vesicles and with muscle cells.

A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.

Sickle cell hemoglobin fiber structure altered by alpha-chain mutation.

Antibodies to rabbit progesterone receptor: crossreaction with human receptor.

ATP-induced formation of an associated complex between microtubules and neurofilaments.

Structure of chromatin and the linking number of DNA.

Field desorption mass spectrometry of oligosaccharides.

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Specific binding of eukaryotic initiation factor 2 to satellite tobacco necrosis virus RNA at a 5′-terminal sequence comprising the ribosome binding site

Flow kinetics of mouse histocompatibility antigens.

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Role of GTP hydrolysis in microtubule treadmilling and assembly.

Method to determine the reading frame of a protein from the purine/pyrimidine genome sequence and its possible evolutionary justification.

Aggregation of colloidal particles modeled as a dynamical process.

NMR studies of 5-hydroxytryptamine transport through large unilamellar vesicle membranes.

Active transport: conditions for linearity and symmetry far from equilibrium.

Acoustic microscopy of living cells.

delta-Aminolevulinic acid synthase from Euglena gracilis.

Eradication of spontaneous metastases and activation of alveolar macrophages by intravenous injection of liposomes containing muramyl dipeptide.

Use of lectins and polyethylene glycol for fusion of glycolipid-containing liposomes with eukaryotic cells.

p53 transformation-related protein: detection by monoclonal antibody in mouse and human cells.

Quantitative colony method for tumorigenic cells transformed by two distinct strains of Friend leukemia virus.

Down regulation of specific binding of [20-3H]phorbol 12,13-dibutyrate and phorbol ester-induced differentiation of human promyelocytic leukemia cells.

Preferential replication of repeated DNA sequences in nuclei isolated from soybean cells grown in suspension culture.

Liponomic control of Ca2+ transport: relationship to mechanism of action of 1,25-dihydroxyvitamin D3.

Chromosome segregation is frequently associated with the expression of recessive mutations in mouse cells.

Malate dehydrogenase: viability of cytosolic nulls and lethality of mitochondrial nulls in maize.

Molecular weight of the membrane C5b-9 complex of human complement: characterization of the terminal complex as a C5b-9 monomer.

Identification of classical anaphylatoxin as the des-Arg form of the C5a molecule: evidence of a modulator role for the oligosaccharide unit in human des-Arg74-C5a.

Permeability characteristics of complement-damaged membranes: evaluation of the membrane leak generated by the complement proteins C5b-9.

Magnetic modulation of release of macromolecules from polymers.

Clastogenic activity from Bloom syndrome fibroblast cultures.

Use of anionic liposomes for the reduction of chronic doxorubicin-induced cardiotoxicity.

Photoisomerized bilirubin in blood from infants receiving phototherapy.

Elliptical erythrocyte membrane skeletons and heat-sensitive spectrin in hereditary elliptocytosis.

Effect of chronologic age on induction of cystathionine synthase, uroporphyrinogen I synthase, and glucose-6-phosphate dehydrogenase activities in lymphocytes.

Auditory brainstem response in dolphins.

Sexually dimorphic brain and behavioral asymmetries in the neonatal rat.

Two adrenal opioid polypeptides: proposed intermediates in the processing of proenkephalin.

Antigen-presenting cell function in induction of helper T cells for cytotoxic T-lymphocyte responses: evidence for antigen processing.

T cells respond preferentially to antigens that are similar to self H-2.

Antigen-specific human T-cell clones: development of clones requiring HLA-DR-compatible presenting cells for stimulation in presence of antigen.

Synaptic localization of alpha-bungarotoxin binding which blocks nicotinic transmission at frog sympathetic neurons.

Human insulin prepared by recombinant DNA techniques and native human insulin interact identically with insulin receptors.

Tritium exchange on transfer RNA: slowly exchanging protons sensitive to a change in the dihydrouridine stem.

Conformational activation of the yeast phenylalanyl-tRNA synthetase catalytic site induced by tRNAPhe interaction: triggering of adenosine or CpCpA trinucleoside diphosphate aminoacylation upon binding of tRNAPhe lacking these residues.

Identification of a second Escherichia coli groE gene whose product is necessary for bacteriophage morphogenesis.

Isolation, culture, and immunocytochemical characterization of epididymal epithelial cells from pubertal and adult rats.

Modulation of fertilization in vitro by peptides released during hamster sperm-zona pellucida interaction.

A mutation downstream from the signal peptidase cleavage site affects cleavage but not membrane insertion of phage coat protein.

Sulfated and nonsulfated glycosaminoglycans and glycopeptides are synthesized by kidney in vivo and incorporated into glomerular basement membranes.

Evidence that rnmB is the operator of the Escherichia coli recA gene.

Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.

Complementation of the plaque-forming cell responses of T-cell-deficient nude mice by a T-cell hybridoma.

Enhancement of formation of the esophageal carcinogen benzylmethylnitrosamine from its precursors by Candida albicans.

Enzyme-linked sandwich immunoassay for insulin using laser fluorimetric detection.

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Acoustic microscopy of living cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Quate, C. F. Johnston, R. N. Rugar, D. Hildebrand, J. A.
Abstract	This paper reports preliminary results of the observation by acoustic microscopy of living cells in vitro. The scanning acoustic microscope uses high-frequency sound waves to produce images with submicrometer resolution. The contrast observed in acoustic micrographs of living cells depends on the acoustic properties (i.e., density, stiffness, and attenuation) and on the topographic contour of the cell. Variation in distance separating the acoustic lens and the viewed cell also has a profound effect on the image. When the substratum is located at the focal plane, thick regions of the cell show a darkening that can be related to cellular acoustic attenuation (a function of cytoplasmic viscosity). When the top of the cell is placed near the focal plane, concentric bright and dark rings appear in the image. The location of the rings can be related to cell topography, and the ring contrast can be correlated to the stiffness and density of the cell. In addition, the character of the images of single cells varies dramatically when the substratum upon which they are grown is changed to a different material. By careful selection of the substratum, the information content of the acoustic images can be increased. Our analysis of acoustic images of actively motile cells indicates that leading lamella are less dense or stiff than the quiescent trailing processes of the cells.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	78
Language	English
Publisher	National Academy of Sciences
Publisher Date	1981-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Myocardium Cytology Animals Cells, Cultured Chick Embryo Heart Ventricles Microscopy Instrumentation Ultrasonics Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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