NDLI: Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.

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Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sharp, J. A. Summers, W. C. Wagner, M. J.
Abstract	We have determined the complete nucleotide sequence of the thymidine kinase (ATP:thymidine 5' phosphotransferase, EC 2.7.1.21) gene of herpes simplex virus type 1 strain CL101 from a plasmid clone of viral DNA derived by Enquist et al. [Enquist, L. W., Vande Woude, G. F., Wagner, M., Smiley, J. R. & Summers, W. C. (1979) Gene 7, 335-342]. A cDNA copy of the 5' end of thymidine kinase mRNA was also analyzed to locate the transcribed sequences. The transcribed portion of the gene is approximately 1300 nucleotides in length and appears to contain no intervening sequences. There is an untranslated region of 107 nucleotides at the 5' end of the mRNA followed by an open reading frame of 1128 nucleotides. The gene is thus capable of coding for a protein of 376 amino acids. Sequences similar to those thought to be involved in control of transcription and translation of a variety of eukaryotic and viral genes such as a 'Hogness box' and A-A-T-A-A-A polyadenylylation signals are also present in the herpesvirus thymidine kinase gene.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	78
Language	English
Publisher	National Academy of Sciences
Publisher Date	1981-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA, Viral Genetics Genes, Viral Simplexvirus Enzymology Thymidine Kinase Amino Acid Sequence DNA Restriction Enzymes RNA, Messenger Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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