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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ryan, D. E. Reik, L. Levin, W. Thomas, P. E. Bresnick, E. Brosseau, M. |
| Abstract | Poly(A)+-RNA obtained from the livers of 3-methylcholanthrene (3MC)-treated rats was translated into cytochrome P-450c in a cell-free reticulocyte system. In this translational system, no precursor cytochrome P-450c was observed. The mRNA responsible for the synthesis of this cytochrome was isolated by immunoprecipitation of liver polyribosomes obtained at 15 hr after 3MC treatment, and a cDNA was constructed by the reverse transcriptase reaction. The cDNA was further purified by hybridizing at a high R0t (product of RNA concentration and incubation time) to poly(A)+-RNA isolated from control rat liver, and the nonhybridized, single-stranded cDNA was isolated by hydroxylapatite chromatography. This cDNAp-450c was employed in hybridization reactions with poly(A)+-RNA isolated from the livers of rats treated with 3MC for various times. These studies indicated a maximal induction of mRNAp-450c at about 15 hr after 3MC injection, although levels of this mRNA were significantly increased by 7 hr. The mRNAp-450c concentration had diminished by 24 hr but remained higher than control levels for at least 48 hr. These studies establish an effect of 3MC upon the accumulation of mRNAp-450c in rat liver. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 7 |
| Volume Number | 78 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1981-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cytochrome P-450 Enzyme System Genetics Methylcholanthrene Pharmacology RNA, Messenger Animals Biosynthesis Enzyme Induction Drug Effects Nucleic Acid Hybridization Protein Biosynthesis Rats, Inbred Strains Time Factors Transcription, Genetic Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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