NDLI: Proenkephalin mRNA in rat heart.

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Proenkephalin mRNA in rat heart.

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Proenkephalin mRNA in rat heart.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Udenfriend, S. Howells, R. D. Kilpatrick, D. L. Noe, M. Bailey, L. C.
Abstract	The distribution of preproenkephalin mRNA in rat tissues was investigated using a homologous cDNA probe for detection. The heart was found to contain larger amounts of the mRNA than any other tissue including brain, which heretofore had been considered the richest source. The identity of the message in heart was verified by hybridizing RNA blots with a synthetic oligodeoxynucleotide that recognizes a different region of the preproenkephalin mRNA sequence than does the cDNA probe. The preproenkephalin mRNA extracted from both heart and brain contained approximately equal to 1500 bases. Dissection of heart revealed that essentially all of the message is contained within the ventricles. In contrast to the large amounts of preproenkephalin mRNA in rat heart, the opioid peptide contents is only 3% of the amount in brain. The rat heart may be a useful model for the investigation of translation control of protein synthesis.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	6
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Enkephalins Biosynthesis Myocardium Protein Precursors RNA, Messenger Animals Brain Chemistry Chromatography, Gel DNA Genetics Isolation & Purification Genetic Markers Heart Ventricles Nucleic Acid Hybridization Oligonucleotides Chemical Synthesis Protein Biosynthesis Protein Processing, Post-Translational Rats, Inbred Strains Spinal Cord Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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