NDLI: Expression of the protein encoded by the 3' open reading frame of human T-cell lymphotropic virus type III in bacteria: demonstration of its immunoreactivity with human sera.

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Expression of the protein encoded by the 3' open reading frame of human T-cell lymphotropic virus type III in bacteria: demonstration of its immunoreactivity with human sera.

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Expression of the protein encoded by the 3' open reading frame of human T-cell lymphotropic virus type III in bacteria: demonstration of its immunoreactivity with human sera.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Robert-guroff, M. Franchini, G. Chang, N. T. Chang, T. W. Wong-staal, F. Ghrayeb, J. Kato, I.
Abstract	The genome of human T-cell lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV), the infectious agent etiologically associated with the acquired immunodeficiency syndrome, contains, in addition to the genes for the polymerase, core, and envelope proteins, several open reading frames. To investigate whether the 3' open reading frame (3' orf) located between the envelope gene and the 3' long terminal repeat is a gene expressed in vivo in infected individuals, we inserted a fragment of 3' orf in a prokaryotic expression vector. The protein product synthesized in bacteria was purified and allowed to react with sera from individuals infected with human T-cell lymphotropic virus type III as indicated by seropositivity for other viral proteins. Two-thirds of the sera, regardless of the clinical status of the individuals, reacted with the purified protein indicating that 3' orf is a viral gene the product of which is immunogenic in vivo. A polyclonal rabbit antiserum reacting against the 3' orf gene product was obtained by serial injection of rabbits with the purified bacterial protein. The antiserum recognized a 27-kDa protein in the human T-cell lymphotropic virus type III-infected lymphocytes.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	14
Volume Number	83
Language	English
Publisher	National Academy of Sciences
Publisher Date	1986-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacterial Proteins Biosynthesis Deltaretrovirus Genetics Genes, Viral Retroviridae Proteins Acquired Immunodeficiency Syndrome Blood Immunology Antigens, Viral Immune Sera Recombinant Proteins T-Lymphocytes Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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