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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rouzer, C. A. Samuelsson, B. |
| Description | Author Affiliation: Rouzer CA ( Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.); |
| Abstract | Maximal activity of human leukocyte 5-lipoxygenase requires Ca2+, ATP, a microsomal membrane preparation, and two cytosolic stimulatory factors. We report here some effects of Ca2+ on the physical properties of the 5-lipoxygenase. When leukocytes were homogenized in the presence of 2 mM EDTA, 5-lipoxygenase was found to be a soluble enzyme. However, when Ca2+ was added to homogenization buffers at 0-1 mM in excess of EDTA, increasing quantities of the enzyme were recovered in the microsomal membrane fraction (100,000 X g pellet). The membrane-associated enzyme was resolubilized by washing pellet preparations in buffers containing 2 mM EDTA and was partially purified by anion-exchange chromatography. Studies of the stimulatory-factor requirements of the membrane-associated, resolubilized, and partially purified enzyme indicated that one of the cytosolic 5-lipoxygenase stimulatory factors exhibited a reversible, Ca2+-dependent membrane association, similar to that of the enzyme itself. Ca2+ also caused a destabilization of the 5-lipoxygenase. Homogenates prepared in the presence of Ca2+ contained lower total enzyme activity, and retention of activity in these samples over time was also diminished. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 21 |
| Volume Number | 84 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1987-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Arachidonate 5-Lipoxygenase Blood Arachidonate Lipoxygenases Calcium Pharmacology Leukocytes Enzymology Isolation & Purification Calcimycin Cell Membrane Chromatography, High Pressure Liquid Chromatography, Ion Exchange Kinetics Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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