NDLI: Aggregation of IgE receptors induces degranulation in rat basophilic leukemia cells permeabilized with alpha-toxin from Staphylococcus aureus.

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Aggregation of IgE receptors induces degranulation in rat basophilic leukemia cells permeabilized with alpha-toxin from Staphylococcus aureus.

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Aggregation of IgE receptors induces degranulation in rat basophilic leukemia cells permeabilized with alpha-toxin from Staphylococcus aureus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hohman, R. J.
Description	Author Affiliation: Hohman RJ ( National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.);
Abstract	A method has been developed by which rat basophilic leukemia (RBL) cells can be permeabilized to small molecules while maintaining their ability to degranulate in response to aggregation of IgE receptors. alpha-Toxin was isolated from culture supernatants of Staphylococcus aureus by precipitation with (NH4)2SO4 and chromatography on phenyl-Sepharose. The isolated toxin binds to the plasma membrane of RBL cells and polymerizes to form a transmembrane pore that allows small molecules (Mr less than 1000), but not macromolecules, to diffuse freely across the membrane. There was no spontaneous release of the contents of RBL cell secretory granules during permeabilization or subsequent incubations. Substantial IgE receptor-mediated exocytosis occurred in the absence of Ca2+, and degranulation was maximal at 0.1-1.0 microM Ca2+, the physiologically important range of Ca2+ concentrations. Using these permeabilized cells, small molecules (i.e., substrates and inhibitors of various enzymes) normally excluded by the plasma membrane can be introduced into the cell. Moreover, the intracellular concentration of ions (such as Ca2+) can be precisely controlled. This method will allow a detailed examination of the individual biochemical events involved in degranulation of mast cells.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	85
Language	English
Publisher	National Academy of Sciences
Publisher Date	1988-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacterial Toxins Pharmacology Basophils Physiology Hemolysin Proteins Receptors, Fc Serotonin Secretion Animals Calcium Cell Membrane Permeability Exocytosis Histamine Release Inositol Metabolism Potassium Chloride Receptors, IgE Staphylococcus Aureus Tumor Cells, Cultured Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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