NDLI: Treponema pallidum invades intercellular junctions of endothelial cell monolayers.

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Treponema pallidum invades intercellular junctions of endothelial cell monolayers.

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Treponema pallidum invades intercellular junctions of endothelial cell monolayers.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Haake, D. A. Navab, M. Lovett, M. A. Thomas, D. D. Fogelman, A. M. Miller, J. N.
Description	Author Affiliation: Thomas DD ( Department of Microbiology and Immunology, University of California, Los Angeles 90024.);
Abstract	The pathogenesis of syphilis reflects invasive properties of Treponema pallidum, but the actual mode of tissue invasion is unknown. We have found two in vitro parallels of treponemal invasiveness. We tested whether motile T. pallidum could invade host cells by determining the fate of radiolabeled motile organisms added to a HeLa cell monolayer; 26% of treponemes associated with the monolayer in a trypsin-resistant niche, presumably between the monolayer and the surface to which it adhered, but did not attain intracellularity. Attachment of T. pallidum to cultured human and rabbit aortic and human umbilical vein endothelial cells was 2-fold greater than to HeLa cells. We added T. pallidum to aortic endothelial cells grown on membrane filters under conditions in which tight intercellular junctions had formed. T. pallidum was able to pass through the endothelial cell monolayers without altering tight junctions, as measured by electrical resistance. In contrast, heat-killed T. pallidum and the nonpathogen Treponema phagedenis biotype Reiter failed to penetrate the monolayer. Transmission electron micrographs of sections of the monolayer showed T. pallidum in intercellular junctions. Our in vitro observations suggest that these highly motile spirochetes may leave the circulation by invading the junctions between endothelial cells.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	85
Language	English
Publisher	National Academy of Sciences
Publisher Date	1988-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Endothelium, Vascular Microbiology Intercellular Junctions Treponema Pallidum Pathogenicity Animals Aorta Cells, Cultured Ultrastructure Epithelium HeLa Cells Kinetics Microscopy, Electron Rabbits Umbilical Veins Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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