NDLI: Carcinoembryonic antigen is anchored to membranes by covalent attachment to a glycosylphosphatidylinositol moiety: identification of the ethanolamine linkage site.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1988 Volume: 85 Issue: 24

Year: 1988 Volume: 85 Issue: 23

Year: 1988 Volume: 85 Issue: 22

Year: 1988 Volume: 85 Issue: 21

Year: 1988 Volume: 85 Issue: 20

Year: 1988 Volume: 85 Issue: 19

Year: 1988 Volume: 85 Issue: 18

Year: 1988 Volume: 85 Issue: 17

Year: 1988 Volume: 85 Issue: 16

Year: 1988 Volume: 85 Issue: 15

Year: 1988 Volume: 85 Issue: 14

Year: 1988 Volume: 85 Issue: 13

Identification of a region in the S1 subunit of pertussis toxin that is required for enzymatic activity and that contributes to the formation of a neutralizing antigenic determinant.

RNase PH: an Escherichia coli phosphate-dependent nuclease distinct from polynucleotide phosphorylase.

Purification and characterization of the human interferon-gamma receptor from placenta.

Structural characterization of the core of the paired helical filament of Alzheimer disease.

Purification and subunit structure of a putative K+-channel protein identified by its binding properties for dendrotoxin I.

Model for how type I restriction enzymes select cleavage sites in DNA.

Differential regulation of S6 phosphorylation by insulin and epidermal growth factor in Swiss mouse 3T3 cells: insulin activation of type 1 phosphatase.

Specificity of tumor necrosis factor toxicity for human mammary carcinomas relative to normal mammary epithelium and correlation with response to doxorubicin.

Isolation of gene fusions (soi::lacZ) inducible by oxidative stress in Escherichia coli.

An intermediate in transposition of the conjugative transposon Tn916.

Cytoplasmic domain affects membrane expression and function of an Ia molecule.

Recombinant human interferon-gamma reconstitutes defective phagocyte function in patients with chronic granulomatous disease of childhood.

Structure and synthesis of a potent glutamate receptor antagonist in wasp venom.

In vitro expression in eukaryotic cells of a prion protein gene cloned from scrapie-infected mouse brain.

Nuclear posttranscriptional processing of thymidine kinase mRNA at the onset of DNA synthesis.

Hox-5.1 defines a homeobox-containing gene locus on mouse chromosome 2.

Lack of evidence for association of meiotic nondisjunction with particular DNA haplotypes on chromosome 21.

Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae.

Requirement of GTP on somatostatin-induced K+ current in human pituitary tumor cells.

Expression of receptors for cholecystokinin and other Ca2+-mobilizing hormones in Xenopus oocytes.

Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae.

Molecular cloning of the common acute lymphoblastic leukemia antigen (CALLA) identifies a type II integral membrane protein.

Wheat germ agglutinin-positive cells in a stem cell-enriched fraction of mouse bone marrow have potent natural suppressor activity.

Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins.

Antiviral agents targeted to interact with viral capsid proteins and a possible application to human immunodeficiency virus.

Transcriptional activation of HLA-DR alpha by interferon gamma requires a trans-acting protein.

Pentose pathway in human liver.

Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells.

Reduced CO2/O2 specificity of ribulose-bisphosphate carboxylase/oxygenase in a temperature-sensitive chloroplast mutant of Chlamydomonas.

Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.

Theileria parva infection induces autocrine growth of bovine lymphocytes.

Evolution of parasitism: kinetoplastid protozoan history reconstructed from mitochondrial rRNA gene sequences. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 85 Issue: 13)

Thermostable DNA polymerase chain amplification of t(14;18) chromosome breakpoints and detection of minimal residual disease.

Antibody specific to the alpha subunit of the guanine nucleotide-binding regulatory protein Go: developmental appearance and immunocytochemical localization in brain.

Effect of in vitro DNA methylation on beta-globin gene expression.

Identification of another member of the transforming growth factor type beta gene family.

Amplified human MYC oncogenes localized to replicating submicroscopic circular DNA molecules.

Transforming growth factor beta stimulates collagen-matrix contraction by fibroblasts: implications for wound healing.

Identification and sequencing of cDNA clones encoding the granule-associated serine proteases granzymes D, E, and F of cytolytic T lymphocytes.

Self-protection of cytotoxic lymphocytes: a soluble form of homologous restriction factor in cytoplasmic granules.

Molecular cloning of a cDNA encoding the human Sm-D autoantigen.

Human renal carcinoma expresses two messages encoding a parathyroid hormone-like peptide: evidence for the alternative splicing of a single-copy gene.

One-dimensional diffusion of Escherichia coli DNA-dependent RNA polymerase: a mechanism to facilitate promoter location.

FLP recombinase is an enzyme.

Posttranslational modification of the Ha-ras oncogene protein: evidence for a third class of protein carboxyl methyltransferases.

Active human immunodeficiency virus protease is required for viral infectivity.

The copper, zinc-superoxide dismutase gene of Saccharomyces cerevisiae: cloning, sequencing, and biological activity.

Human HST1 (HSTF1) gene maps to chromosome band 11q13 and coamplifies with the INT2 gene in human cancer.

Number of visits to a state in a random walk, before absorption, and related topics.

Tunichlorin: a nickel chlorin isolated from the Caribbean tunicate Trididemnum solidum.

Nonenzymatic sequence-specific methyl transfer to single-stranded DNA.

DNA twisting and the affinity of bacteriophage 434 operator for bacteriophage 434 repressor.

Carcinoembryonic antigen is anchored to membranes by covalent attachment to a glycosylphosphatidylinositol moiety: identification of the ethanolamine linkage site.

A Holliday recombination intermediate is twofold symmetric.

Localization and conditional redundancy of regulatory elements in rbcS-3A, a pea gene encoding the small subunit of ribulose-bisphosphate carboxylase.

Characterization of specific pancreatic polypeptide receptors on basolateral membranes of the canine small intestine.

DNA methylation as a mechanism of transcriptional regulation in nonphotosynthetic plastids in plant cells.

Identification of rat growth hormone genomic sequences targeting pituitary expression in transgenic mice.

Sources and evolution of human Alu repeated sequences.

A fundamental division in the Alu family of repeated sequences.

Concentration-affinity equivalence in gene regulation: convergence of genetic and environmental effects.

Anti-tumor effects of antibody-alkaline phosphatase conjugates in combination with etoposide phosphate.

Biological activity of human-mouse IgG1, IgG2, IgG3, and IgG4 chimeric monoclonal antibodies with antitumor specificity.

Regulation of protein kinase C by extracellular calcium in bovine parathyroid cells.

Circulating complexes containing IgA and fibronectin in patients with primary IgA nephropathy.

Increase in albumin, IgG, and IgM blood-nerve barrier indices in human diabetic neuropathy.

Recombinant human insulin-like growth factor I stimulates growth and has distinct effects on organ size in hypophysectomized rats.

Transmission studies from blood of Alzheimer disease patients and healthy relatives.

Magnetic fields and orientation in homing pigeons: experiments of the late W. T. Keeton.

Retinal afferent arborization patterns, dendritic field orientations, and the segregation of function in the lateral geniculate nucleus of the monkey.

cDNA cloning of human liver monoamine oxidase A and B: molecular basis of differences in enzymatic properties.

Year: 1988 Volume: 85 Issue: 12

Year: 1988 Volume: 85 Issue: 11

Year: 1988 Volume: 85 Issue: 10

Year: 1988 Volume: 85 Issue: 9

Year: 1988 Volume: 85 Issue: 8

Year: 1988 Volume: 85 Issue: 7

Year: 1988 Volume: 85 Issue: 6

Year: 1988 Volume: 85 Issue: 5

Year: 1988 Volume: 85 Issue: 4

Year: 1988 Volume: 85 Issue: 3

Year: 1988 Volume: 85 Issue: 2

Year: 1988 Volume: 85 Issue: 1

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Carcinoembryonic antigen is anchored to membranes by covalent attachment to a glycosylphosphatidylinositol moiety: identification of the ethanolamine linkage site.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Paxton, R. J. Hefta, L. J. Hefta, S. A. Lee, T. D. Shively, J. E.
Description	Author Affiliation: Hefta SA ( Division of Immunology, Beckman Research Institute of the City of Hope, Duarte, CA 91010.);
Abstract	The COOH-terminal amino acid of carcinoembryonic antigen (CEA) is shown to covalently link with ethanolamine, evidence consistent with the anchorage of CEA to the plasma membrane through a phosphatidylinositol-glycan tail. Purified CEA was digested with trypsin, and the resulting peptides were isolated by reverse-phase HPLC. Tryptic hexapeptide T12, terminating atypically with alanine, corresponded in sequence (Ser-Ile-Thr-Val-Ser-Ala) with the last six residues (637-642) of the third repeating domain in the mature CEA protein. Mass determination of the hexapeptide by fast atom bombardment mass spectrometry suggested the presence of an additional ethanolamine moiety. This finding and the absence of the subsequent 26 hydrophobic residues predicted by cDNA sequence is evidence that hexapeptide T12 is the COOH-terminal peptide of mature CEA. A synthetic peptide identical to hexapeptide T12 was prepared, and ethanolamine was coupled to its COOH-terminal alanine; chromatographic properties of this synthetic ethanolamine-coupled peptide and peptide T12 were the same. B/E-linked-scan mass spectral analysis of the ethanolamine-coupled synthetic peptide and peptide T12 revealed a fragment ion series consistent with the presence of a COOH-terminal ethanolamine. Release of membrane-bound CEA from the CEA-expressing cell line LS 174T was shown by indirect immunofluorescence and flow cytometry after treatment with phosphatidylinositol-specific phospholipase C. We conclude that CEA is processed posttranslationally to remove the hydrophobic COOH-terminal residues (643-668) with subsequent addition of an ethanolamine-glycosylphosphatidylinositol moiety and that treatment of a colonic cell line with phosphatidylinositol-specific phospholipase C releases membrane-bound CEA.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	13
Volume Number	85
Language	English
Publisher	National Academy of Sciences
Publisher Date	1988-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Carcinoembryonic Antigen Metabolism Ethanolamines Membrane Proteins Neoplasm Proteins Phosphatidylinositols Polysaccharides Adenocarcinoma Isolation & Purification Colonic Neoplasms Ethanolamine Glycosylphosphatidylinositols Liver Neoplasms Peptide Mapping Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in