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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Roepe, P. D. Kaback, H. R. |
| Description | Author Affiliation: Roepe PD ( Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110-1199.); |
| Abstract | Lac permease, a polytopic membrane protein from Escherichia coli, has been purified in soluble form by overexpressing the lacY gene by means of the T7 RNA polymerase system. Soluble permease is dissociated from membranes with urea or other chaotropes and appears after the membrane is saturated with newly synthesized permease. Remarkably, this form of the permease appears to remain soluble in phosphate buffer at neutral pH after removal of urea, although it aggregates in a time- and concentration-dependent manner. Importantly, soluble permease behaves as a monomer during size-exclusion chromatography with or without urea, contains less than 3 mol of organic phosphate per mol of protein, and is largely helical. Soluble permease binds p-nitrophenyl alpha-D-galactopyranoside approximately 40% as well as permease in the native environment of the membrane and can be reconstituted into phospholipid vesicles that catalyze lactose counterflow or active transport in response to a membrane potential (interior negative). The results suggest that lac permease can assume a nondenatured conformation in aqueous solution. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 16 |
| Volume Number | 86 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1989-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Escherichia Coli Proteins Escherichia Coli Enzymology Membrane Proteins Isolation & Purification Membrane Transport Proteins Monosaccharide Transport Proteins Symporters Cell Membrane Circular Dichroism Electrophoresis, Polyacrylamide Gel Kinetics Lactose Metabolism Molecular Weight Solubility Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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