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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | York, D. Swan, D. Bohan, C. Jannoun-nasr, R. Luciw, P. A. Srinivasan, A. Benson, J. Schnoll, S. Kalyanaraman, S. Robinson, R. A. |
| Description | Author Affiliation: Srinivasan A ( Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, GA 30333.); |
| Abstract | Human immunodeficiency virus (HIV) type 1, isolated from diverse sources, exhibits genomic diversity. The mechanisms by which the genomic diversity takes place in individuals exposed to multiple virus isolates is yet to be elucidated. Genetic variation, in general, might result from mutagenic events such as point mutations, rearrangements (insertions and deletions), and recombination. In an attempt to evaluate the process of genetic diversity, we designed experiments to analyze recombination between HIV DNAs by using DNA transfection in cell cultures. Here we report the successful recombination between truncated HIV proviral DNAs with an overlap homology of 53 base pairs that leads to the formation of viable hybrid virus. Recombination was also seen between exogenous DNA introduced into cells and homologous HIV sequences resident in the cells. These results indicate that recombination among various HIV isolates may play a significant role in the generation of genetic diversity of HIV. Further, the method used here enables the construction of hybrid HIV genomes to identify the viral determinants responsible for tropism, replication, and cytopathic effects. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 16 |
| Volume Number | 86 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1989-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | HIV-1 Genetics Recombination, Genetic Cell Line DNA, Viral Enzyme-Linked Immunosorbent Assay HIV Antigens HeLa Cells Molecular Sequence Data Plasmids RNA-Directed DNA Polymerase Restriction Mapping Transfection Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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