NDLI: Continuous monitoring of receptor-mediated changes in the metabolic rates of living cells.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1990 Volume: 87 Issue: 24

Year: 1990 Volume: 87 Issue: 23

Year: 1990 Volume: 87 Issue: 22

Year: 1990 Volume: 87 Issue: 21

Year: 1990 Volume: 87 Issue: 20

Year: 1990 Volume: 87 Issue: 19

Year: 1990 Volume: 87 Issue: 18

Year: 1990 Volume: 87 Issue: 17

Year: 1990 Volume: 87 Issue: 16

Year: 1990 Volume: 87 Issue: 15

Year: 1990 Volume: 87 Issue: 14

Year: 1990 Volume: 87 Issue: 13

Year: 1990 Volume: 87 Issue: 12

Year: 1990 Volume: 87 Issue: 11

Year: 1990 Volume: 87 Issue: 10

Corn metabolites affect growth and virulence of Agrobacterium tumefaciens.

BCL2-mediated tumorigenicity of a human T-lymphoid cell line: synergy with MYC and inhibition by BCL2 antisense.

Regulation of alternative splicing in the generation of isoforms of the mouse Ly-5 (CD45) glycoprotein.

Protein synthesis during acquisition of long-term facilitation is needed for the persistent loss of regulatory subunits of the Aplysia cAMP-dependent protein kinase.

Changes in the sialylation and sulfation of secreted thyrotropin in congenital hypothyroidism.

Enhanced c-myc gene expression during forelimb regenerative outgrowth in the young Xenopus laevis.

Enzymatically active lysosomal proteases are associated with amyloid deposits in Alzheimer brain.

Sequence analysis of ripening-related cytochrome P-450 cDNAs from avocado fruit.

Topographical relationship between beta-amyloid and tau protein epitopes in tangle-bearing cells in Alzheimer disease.

Expression of microtubule-associated protein 2 by reactive astrocytes.

Discrimination between activators and nonactivators of the alternative pathway of complement: regulation via a sialic acid/polyanion binding site on factor H.

A cystic fibrosis pancreatic adenocarcinoma cell line.

Rat mitochondrial and cytosolic 3-hydroxy-3-methylglutaryl-CoA synthases are encoded by two different genes.

Molecular basis for dominantly inherited inclusion body beta-thalassemia.

Purification and properties of Myxococcus xanthus C-factor, an intercellular signaling protein.

2-Azido-[32P]NAD+, a photoactivatable probe for G-protein structure: evidence for holotransducin oligomers in which the ADP-ribosylated carboxyl terminus of alpha interacts with both alpha and gamma subunits.

Binding of substrate CO2 to the active site of human carbonic anhydrase II: a molecular dynamics study.

An interferon gamma-regulated protein that binds the interferon-inducible enhancer element of major histocompatibility complex class I genes.

Autogenous regulation of the Bordetella pertussis bvgABC operon.

Trans-dominant negative mutants of Fos and Jun.

Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana.

Transcriptional activation of a conserved sequence element by ras requires a nuclear factor distinct from c-fos or c-jun.

Lesion-induced increase in nerve growth factor mRNA is mediated by c-fos.

Correction of murine mucopolysaccharidosis VII by a human beta-glucuronidase transgene.

High-level production of a functional immunoglobulin heterodimer in a baculovirus expression system.

Different configurational states of beta-amyloid and their distributions relative to plaques and tangles in Alzheimer disease.

Polyclonal B-cell activation reveals antibodies against human immunodeficiency virus type 1 (HIV-1) in HIV-1-seronegative individuals.

Base changes at position 792 of Escherichia coli 16S rRNA affect assembly of 70S ribosomes.

Activation of ara operons by a truncated AraC protein does not require inducer.

Chromosomal protein HMG-14 gene maps to the Down syndrome region of human chromosome 21 and is overexpressed in mouse trisomy 16.

Enhanced resolution of DNA restriction fragments: a procedure by two-dimensional electrophoresis and double-labeling.

Identification of a serpin-enzyme complex receptor on human hepatoma cells and human monocytes.

NICER elements: a family of nerve growth factor-inducible cAMP-extinguishable retrovirus-like elements.

Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation.

Regulation of glutathione S-transferase Ya subunit gene expression: identification of a unique xenobiotic-responsive element controlling inducible expression by planar aromatic compounds.

Glucocorticoid receptor-dependent disruption of a specific nucleosome on the mouse mammary tumor virus promoter is prevented by sodium butyrate.

Alkylation interference identifies essential DNA contacts for sequence-specific binding of the eukaryotic transcription factor C/EBP.

Continuous monitoring of receptor-mediated changes in the metabolic rates of living cells.

Tissue-specific expression of silkmoth chorion genes in vivo using Bombyx mori nuclear polyhedrosis virus as a transducing vector.

Existence of two forms of rat liver arginyl-tRNA synthetase suggests channeling of aminoacyl-tRNA for protein synthesis.

Transfection of beta-casein chimeric gene and hormonal induction of its expression in primary murine mammary epithelial cells.

Repopulation of the atrophied thymus in diabetic rats by insulin-like growth factor I.

The gamma subunit of DNA polymerase III holoenzyme of Escherichia coli is produced by ribosomal frameshifting.

Reciprocal expression of human ETS1 and ETS2 genes during T-cell activation: regulatory role for the protooncogene ETS1.

Transforming growth factor beta 1 suppression of c-myc gene transcription: role in inhibition of keratinocyte proliferation.

Human mast cell tryptase: multiple cDNAs and genes reveal a multigene serine protease family.

Ultrasensitivity and heavy-metal selectivity of the allosterically modulated MerR transcription complex.

Human immunodeficiency virus causes mononuclear phagocyte dysfunction.

Asymmetric distribution of dystrophin in developing and adult Torpedo marmorata electrocyte: evidence for its association with the acetylcholine receptor-rich membrane.

Escherichia coli thymidylate synthase: amino acid substitutions by suppression of amber nonsense mutations.

Reactivity of primary biliary cirrhosis sera with Escherichia coli dihydrolipoamide acetyltransferase (E2p): characterization of the main immunogenic region.

Human immunodeficiency virus type 1 expression in the central nervous system correlates directly with extent of disease.

Immunization of Aotus monkeys with Plasmodium falciparum blood-stage recombinant proteins.

Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases.

Receptor-mediated endocytosis of transferrin-polycation conjugates: an efficient way to introduce DNA into hematopoietic cells.

Long-range restriction map of the terminal part of the short arm of the human X chromosome.

Asynchronous replication of homologous loci on human active and inactive X chromosomes.

The N-terminal region of the human immunodeficiency virus envelope glycoprotein gp120 contains potential binding sites for CD4.

Identification of the proteolytically activated form of protein kinase C in stimulated human neutrophils.

Refinement of protein dynamic structure: normal mode refinement.

Isolation of cellular promoters by using a retrovirus promoter trap.

Development of a high-titer retrovirus producer cell line capable of gene transfer into rhesus monkey hematopoietic stem cells.

In vitro activation of heat shock transcription factor DNA-binding by calcium and biochemical conditions that affect protein conformation.

Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice.

Effect of controlled adventitial heparin delivery on smooth muscle cell proliferation following endothelial injury.

Pigeons with ablated pyriform cortex home from familiar but not from unfamiliar sites.

Introduction of site-specific mutations into the genome of influenza virus.

DNA intercalation and cleavage of an antitumor antibiotic dynemicin that contains anthracycline and enediyne cores.

Transient calcium release induced by successive increments of inositol 1,4,5-trisphosphate.

A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.

Isolation of the human chromosomal band Xq28 within somatic cell hybrids by fragile X site breakage.

Lipid biophysics of water loss through the skin.

Tandem duplication within a type II collagen gene (COL2A1) exon in an individual with spondyloepiphyseal dysplasia.

Low density lipoprotein rich in oleic acid is protected against oxidative modification: implications for dietary prevention of atherosclerosis.

Hypermutability of CpG dinucleotides in the propeptide-encoding sequence of the human albumin gene.

1,25-Dihydroxy-16-ene-23-yne-vitamin D3 prolongs survival time of leukemic mice.

Molecular characterization of germ-line immunoglobulin A transcripts produced during transforming growth factor type beta-induced isotype switching.

Epidermal growth factor or transforming growth factor alpha is required for kidney tubulogenesis in matrigel cultures in serum-free medium.

Evolutionary changes in influenza B are not primarily governed by antibody selection.

Year: 1990 Volume: 87 Issue: 9

Year: 1990 Volume: 87 Issue: 8

Year: 1990 Volume: 87 Issue: 7

Year: 1990 Volume: 87 Issue: 6

Year: 1990 Volume: 87 Issue: 5

Year: 1990 Volume: 87 Issue: 4

Year: 1990 Volume: 87 Issue: 3

Year: 1990 Volume: 87 Issue: 2

Year: 1990 Volume: 87 Issue: 1

Year: 1990 Volume: 87 Issue: Suppl

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Continuous monitoring of receptor-mediated changes in the metabolic rates of living cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Venter, J. C. Owicki, J. C. Kercso, K. M. Fraser, C. M. Mcconnell, H. M. Parce, J. W. Muir, V. C. Sigal, G. B.
Description	Author Affiliation: Owicki JC ( Molecular Devices Corp., Menlo Park, CA 94025.);
Abstract	Activation of beta-adrenergic or muscarinic acetylcholine receptors expressed in transfected cells or epidermal growth factor receptors in human keratinocytes produces 15% to 200% changes in cellular metabolic rates. Changes in cell metabolism were monitored continuously with a previously described silicon-based microphysiometer that detects small changes in extracellular pH. The amplitude and kinetics of the metabolic changes depend upon several factors including pretreatment of the cells prior to receptor stimulation, the dose of hormone/neurotransmitter used, and the receptor complement of the cells. Responses are receptor specific; cells transfected with receptor genes respond only to the appropriate hormone/transmitter, whereas control (nontransfected) cells or cells transfected with different receptors exhibit no response. The specificity of the responses was further documented by using pharmacological antagonists. In Chinese hamster ovary (CHO) cells transfected with human beta 2-adrenergic receptors, isoproterenol produces a 20-60% increase in the rate of extracellular acidification with an EC50 of 4 nM, a response that is competitively antagonized by (-)-propranolol. The EC50 for the isoproterenol response is shifted from 4 nM to 100 nM in the presence of 3 nM (-)-propranolol. The kinetics of the metabolic response induced by beta-adrenergic receptor stimulation are markedly slower than those elicited by muscarinic receptor agonists. The maximal metabolic response in cells transfected with beta-adrenergic receptors peaks at approximately 12 min as compared with less than 30 sec in cells transfected with muscarinic receptors, perhaps reflecting activation of different second-messenger pathways. These findings illustrate an alternative means of studying cellular responses to hormones and neurotransmitters and suggest that metabolic changes will be generally useful for detecting the consequences of receptor-ligand interactions.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	87
Language	English
Publisher	National Academy of Sciences
Publisher Date	1990-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Epidermal Growth Factor Pharmacology Keratinocytes Metabolism Receptors, Adrenergic, Beta Receptors, Muscarinic Transforming Growth Factors Animals Carbachol Cell Line Cells, Cultured Hydrogen-Ion Concentration Isoproterenol Drug Effects Kinetics L Cells (Cell Line) Mice Genetics Transfection Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in