NDLI: Polyclonal B-cell activation reveals antibodies against human immunodeficiency virus type 1 (HIV-1) in HIV-1-seronegative individuals.

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Polyclonal B-cell activation reveals antibodies against human immunodeficiency virus type 1 (HIV-1) in HIV-1-seronegative individuals.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Powell, J. D. Jehuda-cohen, T. Ahmed-ansari, A. Folks, T. M. Mcclure, H. M. Villinger, F. Slade, B. A. De, B. Sell, K. W.
Description	Author Affiliation: Jehuda-Cohen T ( Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322.);
Abstract	Identification of human immunodeficiency virus type 1 (HIV-1)-infected individuals is of paramount importance for the control of the spread of AIDS worldwide. Currently, the vast majority of screening centers throughout the world rely on serological techniques. As such, clinically asymptomatic but HIV-infected, seronegative individuals are rarely identified. In this report we show that 18% (30/165) of seronegative individuals who were considered to be a unique cohort of patients at high risk for HIV infection had circulating B cells that, upon in vitro polyclonal activation with pokeweed mitogen, produced antibodies reactive with HIV. Furthermore, polymerase chain reaction analysis of DNA obtained from aliquots of the peripheral blood mononuclear cells from these seronegative but pokeweed mitogen assay-positive individuals tested revealed the presence of HIV-specific sequences in a significant number of samples. In addition, depletion of CD8+ T cells from peripheral blood mononuclear cells of HIV-1-seronegative individuals prior to in vitro culture with pokeweed mitogen resulted in increased sensitivity for detecting HIV-reactive antibodies. This assay has obvious epidemiological implications, especially in the case of high-risk groups, and also provides a simple technique to enhance detection of HIV-infected individuals. Of further interest is the determination of the mechanisms related to the lack of HIV-specific antibodies in the serum of these infected individuals.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	87
Language	English
Publisher	National Academy of Sciences
Publisher Date	1990-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	B-Lymphocytes Immunology HIV Antibodies HIV Seropositivity HIV-1 Lymphocyte Activation Antigens, CD8 Antigens, Differentiation, T-Lymphocyte Blotting, Western Cells, Cultured DNA Genetics Isolation & Purification Enzyme-Linked Immunosorbent Assay Pokeweed Mitogens Polymerase Chain Reaction Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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