NDLI: Mutant profiles of selectable genetic elements.

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Geometric interpretation of vertex operator algebras.

Post's program and incomplete recursively enumerable sets.

Tat transactivation of the human immunodeficiency virus type 1 promoter is influenced by basal promoter activity and the simian virus 40 origin of DNA replication.

Interplay between glutamate and gamma-aminobutyric acid transmitter systems in the physiological regulation of brain-derived neurotrophic factor and nerve growth factor synthesis in hippocampal neurons.

Does superoxide underlie the pathogenesis of hypertension?

Clone pAT 133 identifies a gene that encodes another human member of a class of growth factor-induced genes with almost identical zinc-finger domains.

Recombination mediated by vaccinia virus DNA topoisomerase I in Escherichia coli is sequence specific.

Mouse retinoic acid receptor alpha 2 isoform is transcribed from a promoter that contains a retinoic acid response element.

Directed mutagenesis of an iron-sulfur protein of the photosystem I complex in the filamentous cyanobacterium Anabaena variabilis ATCC 29413.

Synchronized overproduction of neurotransmitter receptors in diverse regions of the primate cerebral cortex.

Genomic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates.

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Molecular phylogeny of the superorder Archonta.

Phylogenetic relationships among megabats, microbats, and primates.

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Chronic myeloproliferative disease induced by site-specific integration of Abelson murine leukemia virus-infected hemopoietic stem cells.

Construction of intertypic chimeric dengue viruses by substitution of structural protein genes.

Early mutation of the neu (erbB-2) gene during ethylnitrosourea-induced oncogenesis in the rat Schwann cell lineage.

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Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.

A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals.

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Molecular cloning and characterization of a Ca2+/calmodulin-insensitive adenylyl cyclase from rat brain.

Mutator activity in maize correlates with the presence and expression of the Mu transposable element Mu9.

Pharmacological properties of a potent and selective nonpeptide substance P antagonist.

Agonist-independent activation of acetylcholine receptor channels by protein kinase A phosphorylation.

cAMP-dependent regulation of proenkephalin by JunD and JunB: positive and negative effects of AP-1 proteins.

Expression of acidic and basic fibroblast growth factors in the substantia nigra of rat, monkey, and human.

Thyrotropin-releasing hormone induces opposite effects on Ca2+ channel currents in pituitary cells by two pathways.

Defective membrane expression of human growth hormone (GH) receptor causes Laron-type GH insensitivity syndrome.

Specific lysis of human immunodeficiency virus type 1-infected cells by a HLA-A3.1-restricted CD8+ cytotoxic T-lymphocyte clone that recognizes a conserved peptide sequence within the gp41 subunit of the envelope protein.

Structural requirements for the carbohydrate ligand of E-selectin.

Immunochemical dissection of the Ultrabithorax homeoprotein family in Drosophila melanogaster.

Ligand-stimulated signaling events in immature CD4+CD8+ thymocytes expressing competent T-cell receptor complexes.

Augmentation of synthesis of plasminogen activator inhibitor type 1 by insulin and insulin-like growth factor type I: implications for vascular disease in hyperinsulinemic states.

Com, the phage Mu mom translational activator, is a zinc-binding protein that binds specifically to its cognate mRNA.

Relationship between alternatively spliced exons and functional domains in tropomyosin.

Profound alteration in an alpha beta T-cell antigen receptor repertoire due to polymorphism in the first complementarity-determining region of the beta chain.

Rolling-circle replication of the plasmid pKYM isolated from a gram-negative bacterium. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 88 Issue: 22)

Zinc transfer from transcription factor IIIA fingers to thionein clusters.

Conversion of the E1A Cys4 zinc finger to a nonfunctional His2,Cys2 zinc finger by a single point mutation.

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Characterization of a ceramide-activated protein kinase: stimulation by tumor necrosis factor alpha.

Triple helix formation inhibits transcription elongation in vitro.

Evolution of compensatory substitutions through G.U intermediate state in Drosophila rRNA.

Characterization of G-protein alpha subunits in the Gq class: expression in murine tissues and in stromal and hematopoietic cell lines.

Amino acids 367-376 of the Gs alpha subunit induce membrane association when fused to soluble amino-terminal deleted Gi1 alpha subunit.

Primary structure and functional expression of a glutaminyl cyclase.

Three-dimensional structure of holo 3 alpha,20 beta-hydroxysteroid dehydrogenase: a member of a short-chain dehydrogenase family.

Acetylcholine induces voltage-independent increase of cytosolic calcium in mouse myotubes.

Low-density particles (W-particles) containing catalase in Zellweger syndrome and normal fibroblasts.

DNA sequencing by hybridization: 100 bases read by a non-gel-based method.

Two putative subunits of a peptide pump encoded in the human major histocompatibility complex class II region.

Direct calculation of atomic coordinates from diffraction intensities: space group P1.

A regulated RNA binding protein also possesses aconitase activity.

Molecular genetics of kinesin light chains: generation of isoforms by alternative splicing.

Molecular evolutionary history of ancient aquatic angiosperms.

A role for sunlight in skin cancer: UV-induced p53 mutations in squamous cell carcinoma.

The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix.

Feedback regulation of collagen gene expression: a Trojan horse approach.

Efficient trans cleavage and a common structural motif for the ribozymes of the human hepatitis delta agent.

Cloning and expression of a widely distributed (type IV) adenylyl cyclase.

Cell type-specific integrin variants with alternative alpha chain cytoplasmic domains.

In vivo protein-DNA interactions at the beta-globin gene locus.

Replication of acetylaminofluorene-adducted plasmids in human cells: spectrum of base substitutions and evidence of excision repair.

Evolution of gene position: chromosomal arrangement and sequence comparison of the Drosophila melanogaster and Drosophila virilis sina and Rh4 genes.

Factor-binding element in the human c-myc promoter involved in transcriptional regulation by transforming growth factor beta 1 and by the retinoblastoma gene product.

Rapid activation of hippocampal casein kinase II during long-term potentiation.

Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family.

Suppression of diabetes in nonobese diabetic mice by oral administration of porcine insulin.

High correlation between pentosidine protein crosslinks and pigmentation implicates ascorbate oxidation in human lens senescence and cataractogenesis.

Relative differences in the binding free energies of human immunodeficiency virus 1 protease inhibitors: a thermodynamic cycle-perturbation approach.

The predominant form of the amyloid beta-protein precursor in human brain is protease nexin 2.

Expression of active secreted forms of human amyloid beta-protein precursor by recombinant baculovirus-infected insect cells.

Nuclear matrins: identification of the major nuclear matrix proteins.

Dominant male sterility in mice caused by insertion of a transgene.

Recognition of beta 2-microglobulin-negative (beta 2m-) T-cell blasts by natural killer cells from normal but not from beta 2m- mice: nonresponsiveness controlled by beta 2m- bone marrow in chimeric mice.

A recombinant chimeric plasminogen activator with high affinity for fibrin has increased thrombolytic potency in vitro and in vivo.

Delineation of a previously unrecognized cis-acting element required for HLA class II gene expression.

Expanded distribution of mRNA for nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 in the rat brain after colchicine treatment.

The concept of symmorphosis: a testable hypothesis of structure-function relationship.

A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole.

Four decades of inversion polymorphism in Drosophila pseudoobscura.

Mutant profiles of selectable genetic elements.

Identification and characterization of the neurofibromatosis type 1 protein product.

Centrosomes competent for parthenogenesis in Xenopus eggs support procentriole budding in cell-free extracts.

Compartmentalized expression of a gene under the control of sporulation transcription factor sigma E in Bacillus subtilis.

Peritoneal B cells regulate the numbers of allotype-matched pre-B and B cells in bone marrow.

Identification of the staphylococcal enterotoxin A superantigen binding site in the beta 1 domain of the human histocompatibility antigen HLA-DR.

Molecular cloning, expression, and induction of berberine bridge enzyme, an enzyme essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack.

The vinyl chloride DNA derivative N2,3-ethenoguanine produces G----A transitions in Escherichia coli.

Wild-type p53 can down-modulate the activity of various promoters.

Insulin-like growth factor I shifts from promoting cell division to potentiating maturation during neuronal differentiation.

The small subunit of transcription factor IIF recruits RNA polymerase II into the preinitiation complex.

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Two-dimensional life?

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Mutant profiles of selectable genetic elements.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wurst, H. Pohl, F. M.
Description	Author Affiliation: Wurst H ( Fakultät für Biologie, Universität Konstanz, Federal Republic of Germany.);
Abstract	A method is presented that allows simultaneous analysis of the effects of all possible point mutations within a specific mutation window of at least 50 base pairs on a DNA fragment that codes for a selectable function. It relies on the detection of mismatched base pairs with hydroxylamine and osmium tetroxide. A mutant plasmid library of randomly distributed point mutations within the lacZ' gene of Escherichia coli was selected for functional alpha-complementation by growth on lactose. The DNA fragments of the selected and unselected library were each heat denatured and again renatured, thereby generating a randomly distributed set of all possible mismatches within the mutagenesis window. Cytidine-containing mismatches were then detected with hydroxylamine, and thymidine-containing mismatches were detected with osmium tetroxide. When this procedure was performed for both DNA strands, all mismatches could be detected. A comparison of the results of the unselected and selected library leads to an estimation of the effects of each detectable mutation on alpha-complementation in vivo. This method, called 'mutant profiling,' should be applicable to all selectable genetic elements.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	22
Volume Number	88
Language	English
Publisher	National Academy of Sciences
Publisher Date	1991-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Escherichia Coli Genetics Mutagenesis, Site-Directed Mutation Amino Acid Sequence Base Composition DNA, Bacterial Isolation & Purification Drug Effects Enzymology Gene Library Genetic Complementation Test Hydroxylamine Hydroxylamines Pharmacology Molecular Sequence Data Nucleic Acid Heteroduplexes Oligodeoxyribonucleotides Osmium Tetroxide Plasmids Restriction Mapping Transformation, Bacterial Beta-Galactosidase Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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