NDLI: Detection of intrahepatic replication of hepatitis C virus RNA by in situ hybridization and comparison with histopathology.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1992 Volume: 89 Issue: 24

Year: 1992 Volume: 89 Issue: 23

Year: 1992 Volume: 89 Issue: 22

Year: 1992 Volume: 89 Issue: 21

Year: 1992 Volume: 89 Issue: 20

Year: 1992 Volume: 89 Issue: 19

Year: 1992 Volume: 89 Issue: 18

Year: 1992 Volume: 89 Issue: 17

Year: 1992 Volume: 89 Issue: 16

Year: 1992 Volume: 89 Issue: 15

Year: 1992 Volume: 89 Issue: 14

Year: 1992 Volume: 89 Issue: 13

Year: 1992 Volume: 89 Issue: 12

Year: 1992 Volume: 89 Issue: 11

Year: 1992 Volume: 89 Issue: 10

Year: 1992 Volume: 89 Issue: 9

Year: 1992 Volume: 89 Issue: 8

Year: 1992 Volume: 89 Issue: 7

Year: 1992 Volume: 89 Issue: 6

Axial distances in discrete Möbius groups.

Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures.

Early hydrogen-bonding events in the folding reaction of ubiquitin.

YMXM motifs of IRS-1 define substrate specificity of the insulin receptor kinase.

Differentiation of multiple domains in the herpes simplex virus 1 protease encoded by the UL26 gene.

Homodimerization and constitutive activation of the erythropoietin receptor.

Thrombomodulin gene regulation by cAMP and retinoic acid in F9 embryonal carcinoma cells.

Detection of intrahepatic replication of hepatitis C virus RNA by in situ hybridization and comparison with histopathology.

Multiple retinoid-responsive receptors in a single cell: families of retinoid 'X' receptors and retinoic acid receptors in the Xenopus egg.

Direct effects of luteinizing hormone-releasing hormone agonists and antagonists on MCF-7 mammary cancer cells.

The rat trk protooncogene product exhibits properties characteristic of the slow nerve growth factor receptor.

Relaxin binding in the rat heart atrium.

Crystal structure of the neutral form of fructose 1,6-bisphosphatase complexed with regulatory inhibitor fructose 2,6-bisphosphate at 2.6-A resolution.

Three-dimensional structure of Theiler murine encephalomyelitis virus (BeAn strain).

Differential low density lipoprotein receptor-dependent formation of eicosanoids in human blood-derived monocytes.

Regulatory genes linked to the albino locus in the mouse confer competence for inducible expression on the structural gene encoding serine dehydratase.

Absolute requirement for GTP in activation of human neutrophil NADPH oxidase in a cell-free system: role of ATP in regenerating GTP.

An interaction between p21ras and heat shock protein hsp60, a chaperonin.

Opposing tonically active endogenous opioid systems modulate the mesolimbic dopaminergic pathway.

Cell adhesion molecules as targets for Hox genes: neural cell adhesion molecule promoter activity is modulated by cotransfection with Hox-2.5 and -2.4.

Identification of the promoter of the myelomonocytic leukocyte integrin CD11b.

Extensive genetic diversity in the HLA class II region of Africans, with a focally predominant allele, DRB1*1304.

Characterization of a growth factor that binds exclusively to the erbB-2 receptor and induces cellular responses.

Functional expression of human mdr1 in the yeast Saccharomyces cerevisiae.

ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.

Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.

Induction of nitric oxide synthase activity by toxic shock syndrome toxin 1 in a macrophage-monocyte cell line.

Activation of the cytotactin promoter by the homeobox-containing gene Evx-1.

cDNA cloning of a liver isoform of the phosphorylase kinase alpha subunit and mapping of the gene to Xp22.2-p22.1, the region of human X-linked liver glycogenosis.

Paradoxical transcriptional activation of rat liver cytochrome P-450 3A1 by dexamethasone and the antiglucocorticoid pregnenolone 16 alpha-carbonitrile: analysis by transient transfection into primary monolayer cultures of adult rat hepatocytes.

Molecular cloning and expression of chicken C-terminal Src kinase: lack of stable association with c-Src protein.

Chromosomal position of rearranging gene segments influences allelic exclusion in transgenic mice.

Granule membrane protein 140 (GMP140) binds to carcinomas and carcinoma-derived cell lines.

Isolation and characterization of human cDNA clones encoding a high mobility group box protein that recognizes structural distortions to DNA caused by binding of the anticancer agent cisplatin.

A conditional yeast mutant deficient in mRNA transport from nucleus to cytoplasm.

Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line.

Preproenkephalin promoter 'cassette' confers brain expression and synaptic regulation in transgenic mice.

The Na+/H+ antiporter cytoplasmic domain mediates growth factor signals and controls 'H(+)-sensing'.

Cloning of a D-type cyclin from murine erythroleukemia cells.

Down-regulation of a member of the S100 gene family in mammary carcinoma cells and reexpression by azadeoxycytidine treatment.

Interactions of liver Grp78 and Escherichia coli recombinant Grp78 with ATP: multiple species and disaggregation.

An ATF/CREB binding site is required for virus induction of the human interferon beta gene [corrected].

Laminin-induced retinoblastoma cell differentiation: possible involvement of a 100-kDa cell-surface laminin-binding protein.

A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis.

Neuronal lysosomal enzyme replacement using fragment C of tetanus toxin.

Coagulation factors X, Xa, and protein S as potent mitogens of cultured aortic smooth muscle cells.

The ankyrin repeat domains of the NF-kappa B precursor p105 and the protooncogene bcl-3 act as specific inhibitors of NF-kappa B DNA binding.

Methods and algorithms for statistical analysis of protein sequences.

Mutations in the bZIP domain of yeast GCN4 that alter DNA-binding specificity.

c-fos gene induction by interleukin 2: identification of the critical cytoplasmic regions within the interleukin 2 receptor beta chain.

A retroviral-like metal binding motif in an aminoacyl-tRNA synthetase is important for tRNA recognition.

Heat shock protein hsp70 protects cells from thermal stress even after deletion of its ATP-binding domain.

Cable properties of arborized Retzius cells of the leech in culture as probed by a voltage-sensitive dye.

A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy.

Three-dimensional structure of Theiler virus.

cDNA sequence of a second fibrinogen alpha chain in lamprey: an archetypal version alignable with full-length beta and gamma chains.

Primary structure of Euplotes raikovi pheromones: comparison of five sequences of pheromones from cells with variable mating interactions.

Genetic regulation of human anti-malarial antibodies in twins.

T-cell activation by autologous human T-cell leukemia virus type I-infected T-cell clones.

Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene.

Molecular polymorphism and epidemiology of Neisseria meningitidis immunoglobulin A1 proteases.

A developmentally regulated chlamydial gene with apparent homology to eukaryotic histone H1.

Characterization of NADPH-dependent methemoglobin reductase as a heme-binding protein present in erythrocytes and liver.

Isolation of a candidate gene for choroideremia.

Telomere-related markers for the pseudoautosomal region of the mouse genome.

The interleukin 2 receptor (IL-2R): the IL-2R alpha subunit alters the function of the IL-2R beta subunit to enhance IL-2 binding and signaling by mechanisms that do not require binding of IL-2 to IL-2R alpha subunit.

Expression of functional diphtheria toxin receptors on highly toxin-sensitive mouse cells that specifically bind radioiodinated toxin.

Inactivated whole-virus vaccine derived from a proviral DNA clone of simian immunodeficiency virus induces high levels of neutralizing antibodies and confers protection against heterologous challenge.

Common double- and single-stranded DNA binding factor for a sterol regulatory element.

RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains.

Molecular surface recognition: determination of geometric fit between proteins and their ligands by correlation techniques.

Fte-1, a v-fos transformation effector gene, encodes the mammalian homologue of a yeast gene involved in protein import into mitochondria.

Identification of a shark sequence resembling the major histocompatibility complex class I alpha 3 domain.

Nonrandom distribution of gp120 N-linked glycosylation sites important for infectivity of human immunodeficiency virus type 1.

Increased permeability across the blood-nerve barrier of albumin glycated in vitro and in vivo from patients with diabetic polyneuropathy.

Replication of patient isolates of human immunodeficiency virus type 1 in T cells: a spectrum of rates and efficiencies of entry.

Identification of a specific inhibitor for DNA ligase I in human cells.

Angiogenin supports endothelial and fibroblast cell adhesion.

Malignant transformation of human fibroblast cell strain MSU-1.1 by (+-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo [a]pyrene.

Solution binding of an antigenic peptide to a major histocompatibility complex class I molecule and the role of beta 2-microglobulin.

Chloroquine inhibits intracellular degradation but not secretion of Alzheimer beta/A4 amyloid precursor protein.

Translational frameshifting mediated by a viral sequence in plant cells.

2-Aminopurine overrides multiple cell cycle checkpoints in BHK cells.

Invariant chain can function as a chaperone protein for class II major histocompatibility complex molecules.

Isolation, structure, and activity of -Phe-Met-Arg-Phe-NH2 neuropeptides (designated calliFMRFamides) from the blowfly Calliphora vomitoria.

Point mutations in the dystrophin gene.

Strong evolutionary conservation of neuropeptide Y: sequences of chicken, goldfish, and Torpedo marmorata DNA clones.

A gene encoding a putative tyrosine phosphatase suppresses lethality of an N-end rule-dependent mutant.

Mechanism of maltose transport in Escherichia coli: transmembrane signaling by periplasmic binding proteins.

Analogues of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) as potential anti-platelet-aggregation agents.

Structure and dynamics of des-pentapeptide-insulin in solution: the molten-globule hypothesis.

Rapid detection of inducers of enzymes that protect against carcinogens.

A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure.

Accumulation of cell wall hydroxyproline-rich glycoprotein mRNA is an early event in maize embryo cell differentiation.

Unification of the ferritin family of proteins.

Directed evolution of a protein: selection of potent neutrophil elastase inhibitors displayed on M13 fusion phage.

Participation of bacteriorhodopsin active-site lysine backbone in vibrations associated with retinal photochemistry.

Evidence for regulatory genes on mouse chromosome 7 that affect the quantitative expression of proteins in the fetal and newborn liver.

Enhanced sensitivity of hippocampal pyramidal neurons from mdx mice to hypoxia-induced loss of synaptic transmission.

Extracellular K+ specifically modulates a rat brain K+ channel.

Rhythmogenic effects of weak electrotonic coupling in neuronal models.

Differential accumulation of transcripts for four tomato 1-aminocyclopropane-1-carboxylate synthase homologs under various conditions.

Localization, conjugation, and function of salicylic acid in tobacco during the hypersensitive reaction to tobacco mosaic virus.

Clonal hybrids of the common laboratory fish Fundulus heteroclitus.

Structure of the histidine-containing phosphocarrier protein HPr from Bacillus subtilis at 2.0-A resolution.

Year: 1992 Volume: 89 Issue: 5

Year: 1992 Volume: 89 Issue: 4

Year: 1992 Volume: 89 Issue: 3

Year: 1992 Volume: 89 Issue: 2

Year: 1992 Volume: 89 Issue: 1

On the nature of global classification.

Theory of hydrophobicity: transient cavities in molecular liquids.

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Detection of intrahepatic replication of hepatitis C virus RNA by in situ hybridization and comparison with histopathology.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pacchioni, D. Negro, F. Shimizu, Y. Miller, R. H. Bonino, F. Bussolati, G. Purcell, R. H.
Description	Author Affiliation: Negro F ( Department of Gastroenterology, Ospedale Molinette, Turin, Italy.);
Abstract	A nonisotopic in situ hybridization (NISH) assay was used to detect hepatitis C virus (HCV) RNA. A synthetic oligonucleotide complementary to bases 252-301 of the highly conserved 5' noncoding region of the HCV genome was end-labeled by terminal deoxynucleotidyltransferase using digoxigenin-conjugated dUTP. The hybridized oligomer was revealed by an immunohistochemical reaction after incubation with an alkaline phosphatase-conjugated anti-digoxigenin antibody and subsequent amplification with a complex of alkaline phosphatase and anti-alkaline phosphatase antibodies. The intracellular distribution of HCV RNA was monitored in the livers of two chimpanzees experimentally infected with the H strain of HCV and compared with the serum alanine aminotransferase activity, serum HCV RNA, and liver histopathology. Most cells were stained in the cytoplasm as early as 2 days after inoculation, 1 and 2 days, respectively, before the appearance of viral RNA in the serum. The time course of HCV RNA replication was correlated with increases in serum alanine aminotransferase. However, neither one paralleled the appearance of liver cell necrosis nor showed any correlation with the inflammatory response. The NISH signal was not found in liver biopsy specimens taken from these two animals before inoculation with HCV, from chimpanzees with acute hepatitis type A, B, or delta, or from two animals never experimentally infected with any hepatitis agent; moreover, it disappeared when the positive specimens were predigested with RNase and it was not observed after hybridization of positive controls with a labeled oligomer unrelated to HCV RNA. Thus, detection of liver HCV RNA by NISH is a sensitive and specific method for studying HCV replication at the cellular level. Intracellular replication of HCV did not appear to be associated with histopathologic changes in the liver, although the correlation with increases of liver enzyme activity in the serum suggested possible damage to the liver cell membrane.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	6
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Hepacivirus Physiology Liver Microbiology RNA, Viral Genetics Alanine Transaminase Blood Metabolism Animals Biopsy Isolation & Purification Hepatitis C Pathology Molecular Sequence Data Oligonucleotide Probes Pan Troglodytes Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in