NDLI: Cationic lipids direct a viral glycoprotein into the class I major histocompatibility complex antigen-presentation pathway.

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Cationic lipids direct a viral glycoprotein into the class I major histocompatibility complex antigen-presentation pathway.

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On the nature of global classification.

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Cationic lipids direct a viral glycoprotein into the class I major histocompatibility complex antigen-presentation pathway.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Erickson, A. Van Nest, G. V. Cataldo, D. Walker, C. Selby, M. Valensi, J. P.
Description	Author Affiliation: Walker C ( Chiron Corporation, Emeryville, CA 94608.);
Abstract	Recombinant glycoprotein B (gB) of herpes simplex virus (HSV) was processed and presented by class I major histocompatibility complex (MHC) molecules after delivery into cells by using N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl sulfate (DOTAP), a commercially available cationic lipid used for DNA transfection. Cells treated with DOTAP-associated gB were susceptible to lysis by class I MHC-restricted, HSV-specific cytotoxic T lymphocytes (CTL), and the treated cells restimulated memory gB-specific CTL activity in spleen cells from HSV-infected mice. gB-specific CTL responses were detected in mice immunized with recombinant gB and DOTAP but not in those receiving gB emulsified in complete Freund's adjuvant. Thus, cationic lipids may facilitate induction of CD8+ T-cell responses in vaccinations with recombinant antigens, and they may serve as readily available reagents for dissecting class I MHC immunity to viruses and other intracellular pathogens.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	17
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antigen-Presenting Cells Metabolism Fatty Acids, Monounsaturated Pharmacology Histocompatibility Antigens Class I Quaternary Ammonium Compounds T-Lymphocytes, Cytotoxic Immunology Viral Envelope Proteins Adjuvants, Immunologic Animals Antigens, CD8 Cytotoxicity, Immunologic Immunologic Memory Mice Mice, Inbred C57BL Peptides Vaccines, Synthetic Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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