NDLI: Genome mapping by nonrandom anchoring: a discrete theoretical analysis.

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Genome mapping by nonrandom anchoring: a discrete theoretical analysis.

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Genome mapping by nonrandom anchoring: a discrete theoretical analysis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, M. Q. Marr, T. G.
Description	Author Affiliation: Zhang MQ ( Cold Spring Harbor Laboratory, NY 11724.);
Abstract	As part of our effort to construct a physical map of the genome of the fission yeast Schizosaccharomyces pombe, we have made theoretical predictions for the progress expected, as measured by the expected length fraction of island coverage and by the expected properties of the anchored islands such as the number and the size of islands. Our experimental strategy is to construct a random clone library and screen the library for clones having unique sequence at both ends. This scheme is essentially the same as the clone-limited double sequence-tagged-site selection scheme which was used in a computer simulation by Palazzolo et al. [Palazzolo, M. J., Sawyer, S. A., Martin, C. H., Smoller, D. A. & Hartl, D. L. (1991) Proc. Natl. Acad. Sci. USA 88, 8034-8038]. Both simulation and ongoing experiments in our laboratory have shown that the nonrandom anchoring method is far superior to random anchoring. In this paper, we propose a theoretical model to explain the simulated data and the experimental data.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	90
Language	English
Publisher	National Academy of Sciences
Publisher Date	1993-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Chromosome Mapping Genome, Fungal Models, Genetic Schizosaccharomyces Genetics Sequence Tagged Sites Computer Simulation Gene Library Comparative Study Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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