NDLI: Differentiation and identification of the two catalytic metal binding sites in bovine lens leucine aminopeptidase by x-ray crystallography.

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Physical cosmology. Introduction to papers presented at a colloquium 'Physical Cosmology,' March 27-28, 1992, National Academy of Sciences, Irvine, CA.

Scientific results from the Cosmic Background Explorer (COBE).

Preliminary results from the third flight of the Millimeter Anisotropy Experiment (MAX).

University of california at Santa Barbara Anisotropy Program: degree scale results from the South Pole 1990-1991.

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Cosmological implications of light element abundances: theory.

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K+-conducting ion channel of the chloroplast inner envelope: functional reconstitution into liposomes.

Kinetics of p56lck and p60src Src homology 2 domain binding to tyrosine-phosphorylated peptides determined by a competition assay or surface plasmon resonance.

The Drosophila Stubble-stubbloid gene encodes an apparent transmembrane serine protease required for epithelial morphogenesis.

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Molecular cloning, functional expression, and pharmacological characterization of an N-methyl-D-aspartate receptor subunit from human brain.

Expression cloning of an ATP receptor from mouse neuroblastoma cells.

Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.

Blockade of tolerance to morphine but not to kappa opioids by a nitric oxide synthase inhibitor.

The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors.

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Priming with recombinant influenza virus followed by administration of recombinant vaccinia virus induces CD8+ T-cell-mediated protective immunity against malaria.

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Genetically controlled food preference: biochemical mechanisms.

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Specific labeling and permanent activation of the retinal rod cGMP-activated channel by the photoaffinity analog 8-p-azidophenacylthio-cGMP.

Identification and cloning of a protein kinase-encoding mouse gene, Plk, related to the polo gene of Drosophila.

Potential role of phospholipase A2 in HL-60 cell differentiation to macrophages induced by protein kinase C activation.

Feedback inhibition of fully unadenylylated glutamine synthetase from Salmonella typhimurium by glycine, alanine, and serine.

Unusual organization of the human T-cell receptor beta-chain gene complex is linked to recombination hotspots.

Molecular and active-site structure of a Bacillus 1,3-1,4-beta-glucanase.

Identification of cell-specific and developmentally regulated nuclear factors that direct myeloid and lymphoid expression of the CD11a gene.

Ontogeny of the prohormone convertases PC1 and PC2 in the mouse hypophysis and their colocalization with corticotropin and alpha-melanotropin.

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Origin of JC polyomavirus variants associated with progressive multifocal leukoencephalopathy.

Analysis of the mouse protamine 1 promoter in transgenic mice.

The E1 protein of bovine papilloma virus 1 is an ATP-dependent DNA helicase.

Physical and functional interaction between WT1 and p53 proteins.

Concordance between isolated cleft palate in mice and alterations within a region including the gene encoding the beta 3 subunit of the type A gamma-aminobutyric acid receptor.

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von Willebrand factor mediates platelet adhesion to virally infected endothelial cells.

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Gene organization and primary structure of human hormone-sensitive lipase: possible significance of a sequence homology with a lipase of Moraxella TA144, an antarctic bacterium.

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Colocalization of centromeric and replicative functions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica.

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Elevated glucose and angiotensin II increase 12-lipoxygenase activity and expression in porcine aortic smooth muscle cells.

Echo-planar magnetic resonance imaging studies of frontal cortex activation during word generation in humans.

Nouns and verbs are retrieved with differently distributed neural systems.

p53 gene mutations and protein accumulation in human ovarian cancer.

Axonal sprouting and laminin appearance after destruction of glial sheaths.

Functional heterogeneity of calcium release by inositol trisphosphate in single Purkinje neurones, cultured cerebellar astrocytes, and peripheral tissues.

Differentiation and identification of the two catalytic metal binding sites in bovine lens leucine aminopeptidase by x-ray crystallography.

Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.

Single amino acid substitution affects desensitization of the 5-hydroxytryptamine type 3 receptor expressed in Xenopus oocytes.

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Rapid identification of proteins.

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Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck.

Identification of two missense mutations in a dihydrolipoamide dehydrogenase-deficient patient.

Release of amyloid beta-protein precursor derivatives by electrical depolarization of rat hippocampal slices.

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5'-CGA sequence is a strong motif for homo base-paired parallel-stranded DNA duplex as revealed by NMR analysis.

Transfection of the malaria parasite and expression of firefly luciferase.

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Picosecond infrared studies of the dynamics of the photosynthetic reaction center.

Structural changes in the actomyosin cross-bridges associated with force generation.

Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas.

Myocyte enhancer factor (MEF) 2C: a tissue-restricted member of the MEF-2 family of transcription factors.

Isolation and the complete amino acid sequence of lumenal endoplasmic reticulum glucose-6-phosphate dehydrogenase.

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The beta subunit of the mitochondrial processing peptidase from rat liver: cloning and sequencing of a cDNA and comparison with a proposed family of metallopeptidases.

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Differentiation and identification of the two catalytic metal binding sites in bovine lens leucine aminopeptidase by x-ray crystallography.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, H. Lipscomb, W. N.
Description	Author Affiliation: Kim H ( Gibbs Chemical Laboratory, Harvard University, Cambridge, MA 02138.);
Abstract	The tightly binding and readily exchanging metal binding sites in the active site of bovine lens leucine aminopeptidase (blLAP; EC 3.4.11.1) have been differentiated and identified by x-ray crystallography. In native blLAP,Zn2+ occupies both binding sites. In solution, site 1 readily exchanges Zn2+ for other divalent cations, including Mg2+. The Zn2+ in site 2 is unavailable for metal exchange under conditions which allow exchange at site 1. The Zn2+/Mg2+ metal hybrid of blLAP (Mg-blLAP) was prepared in solution and crystallized. X-ray diffraction data to 2.9-A resolution were collected at -150 degrees C from single crystals of Mg-blLAP and native blLAP. Comparisons of omit maps calculated from the Mg-blLAP data with analogous maps calculated from the native blLAP data show electron density in one of the metal binding sites in Mg-blLAP which is much weaker than the electron density in the other binding site. Since there are fewer electrons associated with Mg2+ than with Zn2+, the difference in electron density between the two metal binding sites is consistent with occupancy of the weaker electron density site by Mg2+ and identifies this metal binding site as site 1, defined as the readily exchanging site. The present identification of the metal binding sites reverses the previous presumptive assignment of the metal binding sites which was based on the structure of native blLAP [Burley, S. K., David, P. R., Sweet, R. M., Taylor, A. & Lipscomb, W. N. (1992) J. Mol. Biol. 224, 113-140]. According to the residue-numbering convention of native blLAP, the new assignment of the metal binding sites identifies the readily exchanging site 1 with Zn-488, which is within interaction distance of one side-chain carboxylate oxygen from each of Asp-255, Asp-332, and Glu-334 and the main-chain carbonyl oxygen of Asp-332. The more tightly binding site 2 is identified with Zn-489, which is within interaction distance of one side-chain carboxylate oxygen from each of Asp-255, Asp-273, and Glu-334 and the side-chain amine nitrogen of Lys-250.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	11
Volume Number	90
Language	English
Publisher	National Academy of Sciences
Publisher Date	1993-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Lens, Crystalline Enzymology Leucyl Aminopeptidase Chemistry Metabolism Magnesium Protein Conformation Zinc Amino Acid Sequence Animals Binding Sites Cations, Divalent Chromatography, Gel Isolation & Purification Models, Structural X-Ray Diffraction Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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