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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, N. Willingham, M. C. Maurizi, M. R. Gottesman, M. M. Gottesman, S. |
| Description | Author Affiliation: Wang N ( Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.); |
| Abstract | We have cloned a human ATP-dependent protease that is highly homologous to members of the bacterial Lon protease family. The cloned gene encodes a protein of 963 amino acids with a calculated molecular mass of 106 kDa, slightly higher than that observed by Western blotting the protein from human tissues and cell lines (100 kDa). A single species of mRNA was found for this Lon protease in all human tissues examined. The protease is encoded in the nucleus, and the amino-terminal portion of the protein sequence contains a potential mitochondrial targeting presequence. Immunofluorescence microscopy suggested a predominantly mitochondrial localization for the Lon protease in cultured human cells. A truncated LON gene, in which translation was initiated at Met118 of the coding sequence, was expressed in Escherichia coli and produced a protease that degraded alpha-casein in vitro in an ATP-dependent manner and had other properties similar to E. coli Lon protease. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 23 |
| Volume Number | 90 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1993-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Escherichia Coli Proteins Genes Heat-Shock Proteins Chemistry Mitochondria Enzymology Protease La Serine Endopeptidases ATP-Dependent Proteases Amino Acid Sequence Blotting, Western Cloning, Molecular DNA, Complementary Genetics Metabolism Molecular Sequence Data Mutagenesis, Site-Directed Sequence Alignment Sequence Homology, Amino Acid Structure-Activity Relationship Tissue Distribution Comparative Study Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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