NDLI: Fluctuation analysis of motor protein movement and single enzyme kinetics.

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Fluctuation analysis of motor protein movement and single enzyme kinetics.

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Fluctuation analysis of motor protein movement and single enzyme kinetics.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Block, S. M. Mitra, P. P. Svoboda, K.
Description	Author Affiliation: Svoboda K ( Rowland Institute for Science, Cambridge, MA 02142.);
Abstract	We studied fluctuations in the displacement of silica beads driven by single molecules of the motor protein kinesin, moving under low mechanical loads at saturating ATP concentrations. The variance in position was significantly smaller than expected for the case of stepwise movement along a regular lattice of positions with exponentially distributed intervals. The small variance suggests that two or more sequential processes with comparable reaction rates dominate the biochemical cycle. The low value is inconsistent with certain recently proposed thermal ratchet models for motor movement as well as with scenarios where the hydrolysis of a single ATP molecule leads to a cluster of several steps. Fluctuation analysis is a potential powerful tool for studying kinetic behavior whenever the output of a single enzyme can be monitored.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	25
Volume Number	91
Language	English
Publisher	National Academy of Sciences
Publisher Date	1994-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Kinesin Metabolism Animals Decapodiformes Chemistry Isolation & Purification Kinetics Mathematics Nervous System Enzymology Probability Time Factors Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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