NDLI: Model study detecting breast cancer cells in peripheral blood mononuclear cells at frequencies as low as 10(-7).

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1995 Volume: 92 Issue: 26

Year: 1995 Volume: 92 Issue: 25

Year: 1995 Volume: 92 Issue: 24

Year: 1995 Volume: 92 Issue: 23

Year: 1995 Volume: 92 Issue: 22

Year: 1995 Volume: 92 Issue: 21

Year: 1995 Volume: 92 Issue: 20

Year: 1995 Volume: 92 Issue: 19

Year: 1995 Volume: 92 Issue: 18

Year: 1995 Volume: 92 Issue: 17

Year: 1995 Volume: 92 Issue: 16

Year: 1995 Volume: 92 Issue: 15

Year: 1995 Volume: 92 Issue: 14

Year: 1995 Volume: 92 Issue: 13

Year: 1995 Volume: 92 Issue: 12

Year: 1995 Volume: 92 Issue: 11

Year: 1995 Volume: 92 Issue: 10

Year: 1995 Volume: 92 Issue: 9

Year: 1995 Volume: 92 Issue: 8

Year: 1995 Volume: 92 Issue: 7

Year: 1995 Volume: 92 Issue: 6

Year: 1995 Volume: 92 Issue: 5

Year: 1995 Volume: 92 Issue: 4

Year: 1995 Volume: 92 Issue: 3

Year: 1995 Volume: 92 Issue: 2

Forced coalescence phasing: a method for ab initio determination of crystallographic phases.

Algebraic aspects of the computably enumerable degrees.

An aphid-resistance locus is tightly linked to the nematode-resistance gene, Mi, in tomato.

Chemoattraction of male gametes by a pheromone produced by female gametes of Chlamydomonas.

T cells with two functional antigen-specific receptors.

Cells of Escherichia coli swim either end forward.

Recent African origin of modern humans revealed by complete sequences of hominoid mitochondrial DNAs.

Active gypsy/Ty3 retrotransposons or retroviruses in Caenorhabditis elegans.

Getting the inside out: the transporter associated with antigen processing (TAP) and the presentation of viral antigen.

Building zinc fingers by selection: toward a therapeutic application.

Human immunodeficiency virus type 1 Nef and p56lck protein-tyrosine kinase interact with a common element in CD4 cytoplasmic tail.

Human spleen tyrosine kinase p72Syk associates with the Src-family kinase p53/56Lyn and a 120-kDa phosphoprotein.

Targeting of a nuclease to murine leukemia virus capsids inhibits viral multiplication.

Solubilization of beta-amyloid-(1-42)-peptide: reversing the beta-sheet conformation induced by aluminum with silicates.

Rapid long-range proton diffusion along the surface of the purple membrane and delayed proton transfer into the bulk.

Lymphoid development in mice with a targeted deletion of the interleukin 2 receptor gamma chain.

Self-association of a synthetic peptide from the N terminus of the hepatitis delta virus protein into an immunoreactive alpha-helical multimer.

A retinoic acid-triggered cascade of HOXB1 gene activation.

Evidence for two distinct retinoic acid response pathways for HOXB1 gene regulation.

Preservation of hemostatic and structural properties of rehydrated lyophilized platelets: potential for long-term storage of dried platelets for transfusion.

Macrophage foam cells from experimental atheroma constitutively produce matrix-degrading proteinases.

Systemin activates synthesis of wound-inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathway.

The orphan receptors COUP-TF and HNF-4 serve as accessory factors required for induction of phosphoenolpyruvate carboxykinase gene transcription by glucocorticoids.

Cooperative binding of lactose and the phosphorylated phosphocarrier protein HPr(Ser-P) to the lactose/H+ symport permease of Lactobacillus brevis.

Cooperative formation of high-order oligomers by retinoid X receptors: an unexpected mode of DNA recognition.

Variability of dopamine D4 receptor (DRD4) gene sequence within and among nonhuman primate species.

Breast and ovarian cancer-specific cytotoxic T lymphocytes recognize the same HER2/neu-derived peptide.

Cyclosporin A blocks apoptosis by inhibiting the DNA binding activity of the transcription factor Nur77.

Angiogenin antagonists prevent tumor growth in vivo.

Avian cellular homolog of the qin oncogene.

A relationship between protein stability and protein function.

Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells.

Bioactive conformation of stromelysin inhibitors determined by transferred nuclear Overhauser effects.

Fibroblast growth factor receptor is required for in vivo cardiac myocyte proliferation at early embryonic stages of heart development.

Functional interaction of yeast and human TATA-binding proteins with an archaeal RNA polymerase and promoter.

Genomic imprinting proposed as a surveillance mechanism for chromosome loss.

Expression of the E2F1 transcription factor overcomes type beta transforming growth factor-mediated growth suppression.

Determination of helix-helix interactions in membranes by rotational resonance NMR.

An autoregulatory region in protein kinase C: the pseudoanchoring site.

A bovine cDNA and a yeast gene (VMA8) encoding the subunit D of the vacuolar H(+)-ATPase.

A cluster of basic amino acids within an alpha-helix is essential for alpha-subunit recognition by the glycoprotein hormone N-acetylgalactosaminyltransferase.

Reaction in alphavirus mRNA capping: formation of a covalent complex of nonstructural protein nsP1 with 7-methyl-GMP.

Visualization of the thyrotropin-releasing hormone receptor and its ligand during endocytosis and recycling.

Leukotriene B4 plays a critical role in the progression of collagen-induced arthritis.

p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p.

Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes.

Model study detecting breast cancer cells in peripheral blood mononuclear cells at frequencies as low as 10(-7).

The neural cell adhesion molecule (N-CAM) inhibits proliferation in primary cultures of rat astrocytes.

Activation of NF-kappa B requires proteolysis of the inhibitor I kappa B-alpha: signal-induced phosphorylation of I kappa B-alpha alone does not release active NF-kappa B.

Periodic recurrence of methionines: fossil of gene fusion?

Activation of the myogenin promoter during mouse embryogenesis in the absence of positive autoregulation.

Effect of erythrocyte membrane on extracellular development of the erythrocytic cycle of Plasmodium falciparum.

Nanosecond time-resolved circular polarization of fluorescence: study of NADH bound to horse liver alcohol dehydrogenase.

The force generated by a single kinesin molecule against an elastic load.

Cell cycle regulation of metallothionein in human colonic cancer cells.

Effects of interferon gamma and major histocompatibility complex-encoded subunits on peptidase activities of human multicatalytic proteases.

Long-term effects of embryo freezing in mice.

Seminalplasmin: recent evolution of another member of the neuropeptide Y gene family.

Two membrane forms of guanylyl cyclase found in the eye.

Pan-neurotrophin 1: a genetically engineered neurotrophic factor displaying multiple specificities in peripheral neurons in vitro and in vivo.

The period clock gene is expressed in central nervous system neurons which also produce a neuropeptide that reveals the projections of circadian pacemaker cells within the brain of Drosophila melanogaster.

Disregulation of leukosialin (CD43, Ly48, sialophorin) expression in the B-cell lineage of transgenic mice increases splenic B-cell number and survival.

Induction of antibodies to the human immunodeficiency virus type 1 by immunization of baboons with immunoglobulin molecules carrying the principal neutralizing determinant of the envelope protein.

Cyanobacterial protein with similarity to the chlorophyll a/b binding proteins of higher plants: evolution and regulation.

Evidence for intron capture: an unusual path for the evolution of proteins. (Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 92 Issue: 2)

Year: 1995 Volume: 92 Issue: 1

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Model study detecting breast cancer cells in peripheral blood mononuclear cells at frequencies as low as 10(-7).

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hoffman, R. A. Verwer, B. Gross, H. J. Recktenwald, D. Houck, D.
Description	Author Affiliation: Gross HJ ( Monoclonal Research Center, Becton Dickinson Immunocytometry Systems, San Jose, CA 95131-1807.);
Abstract	A flow cytometric assay was developed to detect rare cancer cells in blood and bone marrow. Multiple markers; each identified by a separate color of immunofluorescence (yellow and two shades of red), are used to reliably identify the cancer cells. Blood or bone marrow cells, which are not of interest but interfere in detecting the cancer cells, are identified by a panel of immunofluorescence markers, each of which has the same color (green). Thus, the rare cancer cells of interest are yellow and two different shades of red but not green. The requirement that the rare cancer cell be simultaneously positive for three separate colors (the specific markers) and negative for a fourth color (the exclusion color) allowed detection of as few as one cancer cell in 10(7) nucleated blood cells (a frequency of 10(-7). To test this rare-event assay prior to clinical studies, a model study was performed in which the clinical sample was simulated by mixing small numbers of cells from the breast carcinoma line BT-20 with peripheral blood mononuclear cells. We detected statistically significant numbers of BT-20 cells at mixing frequencies of 10(-5), 10(-6), and 10(-7). In control samples, no target events (BT-20) were observed when more than 10(8) cells were analyzed. For additional confirmation that the BT-20 cells in the model study were correctly identified and counted, the BT-20 cells (and only BT-20 cells) were covalently stained with a fifth fluorescence dye, 7-amino-4-chloromethylcoumarin (CMAC). CMAC fluorescence data were not used in the assay for detecting BT-20 cells. Only after the analysis using data from the specific and exclusion colors had been completed were the events identified as BT-20 cells checked for CMAC fluorescence. The putative BT-20 events were always found to be positive for CMAC fluorescence, which further increases confidence in the assay. Manual data analysis and an automated computer program were compared. Results were comparable with the manual and automated methods, but the automated 'genetic algorithm' always found more BT-20 events. Cell sorting of BT-20 cells from samples that contained BT-20 at frequencies of 10(-5), 10(-6), and 10(-7) provided further evidence that these rare cells could be reliably detected. The good performance of the assay with the model system will encourage further studies on clinical samples.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	2
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Breast Neoplasms Pathology Carcinoma Flow Cytometry Neoplastic Cells, Circulating Algorithms Tumor Markers, Biological Diagnosis, Computer-Assisted Fluorescent Antibody Technique Leukocytes, Mononuclear Cytology Neoplasm Metastasis Sensitivity And Specificity Tumor Cells, Cultured Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in