NDLI: Human immunodeficiency virus reverse transcriptase substitutes for DNA polymerase I in Escherichia coli.

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Human immunodeficiency virus reverse transcriptase substitutes for DNA polymerase I in Escherichia coli.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Loeb, L. A. Kim, B.
Description	Author Affiliation: Kim B ( Joseph Gottstein Memorial Cancer Research Laboratory, Department of Pathology, University of Washington, Seattle 98195.);
Abstract	We present evidence that human immunodeficiency virus (HIV) reverse transcriptase (RT) can substitute for DNA polymerase I in bacteria. Expression of HIV RT enables an Escherichia coli mutant, polA12 recA718, containing a temperature-sensitive mutation in DNA polymerase I, to grow at a nonpermissive temperature. The plasmid pBR322 contains a DNA polymerase I-dependent origin of replication. Expression of HIV RT enables the same E. coli mutant to maintain this plasmid at a nonpermissive temperature. Furthermore, expression of HIV RT in this mutant renders it sensitive to 3'-azido-3'-deoxythymidine, a commonly used anti-AIDS drug that targets HIV RT. These combined findings on the genetic complementation of DNA polymerase I by HIV RT provide a bacterial assay to screen for drugs directed against HIV RT. Genetic complementation provides a method for positive selection of large numbers of functional HIV RT mutants for studies on structure-function relationships.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA Polymerase I Metabolism Escherichia Coli Enzymology HIV RNA-Directed DNA Polymerase DNA Replication Drug Effects Drug Evaluation, Preclinical Genetics Genetic Complementation Test Mutation Physiology Plasmids Temperature Zidovudine Pharmacology Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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