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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nürnberger, T. Hahlbrock, K. Nennstiel, D. Scheel, D. |
| Description | Author Affiliation: Nürnberger T ( Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Cologne, Germany.); |
| Abstract | An oligopeptide elicitor from Phytophthora megasperma f.sp. glycinea (Pep-13) that induces phytoalexin accumulation in cultured parsley cells was radioiodinated and chemically cross-linked to its binding site in microsomal and plasma membrane preparations with each of three homobifunctional reagents. Analysis by SDS/PAGE and autoradiography of solubilized membrane proteins demonstrated labeling of a 91-kDa protein, regardless of which reagent was used. Cross-linking of this protein was prevented by addition of excess unlabeled Pep-13. The competitor concentration found to half-maximally reduce the intensity of the cross-linked band was 6 nM, which is in good agreement with the IC50 value of 4.7 nM, obtained from ligand binding assays. No crosslinking of 125I-labeled Pep-13 was observed by using microsomal membranes from three other plant species, indicating species-specific occurrence of the binding site. Coupling of 125I-Pep-13 to the parsley 91-kDa protein required the same structural elements within the ligand as was recently reported for binding of 125I-Pep-13 to parsley microsomes, elicitor-induced stimulation of ion fluxes across the plasma membrane, the oxidative burst, the expression of defense-related genes, and phytoalexin production. These findings suggest that the 91-kDa protein identified in parsley membranes is the oligopeptide elicitor receptor mediating activation of a multicomponent defense response. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 6 |
| Volume Number | 92 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1995-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Angiosperms Metabolism Fungal Proteins Membrane Glycoproteins Phytophthora Succinimides Pharmacology Amino Acid Sequence Binding, Competitive Cells, Cultured Cross-Linking Reagents Daucus Carota Electrophoresis, Polyacrylamide Gel Isolation & Purification Glycoproteins Intracellular Membranes Iodine Radioisotopes Kinetics Membrane Proteins Microsomes Molecular Sequence Data Peptide Fragments Chemistry Plant Extracts Biosynthesis Sesquiterpenes Soybeans Substrate Specificity Terpenes Comparative Study Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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