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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kimura, Y. Stadtman, T. C. |
| Description | Author Affiliation: Kimura Y ( Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.); |
| Abstract | The complete amino acid sequence of Clostridium sticklandii selenoprotein A, a selenocysteine-containing protein component of the glycine reductase complex, has been established. Both the intact protein and peptide fragments produced by Staphylococcus aureus V8 protease or trypsin were purified by reversed-phase high-performance liquid chromatography and subjected to electrospray ionization mass spectrometric analysis and standard Edman degradation. Selenoprotein A consists of 157 amino acids with a chemical molecular weight of 17,011, in reasonable agreement with the observed molecular weight (17,022.7) determined from its ionization mass spectrum. The sequence of the amino-terminal region of the isolated native protein is Ser-Arg-Phe-Thr-Gly-Lys- Lys-Ile-Val-Ile-Ile-Gly-Asp-Arg-Asp-. An N-terminal methionine residue deduced from the gene sequence was not present. Although selenoprotein A reacted positively in a glycoprotein stain when using either the periodic acid-Schiff reagent procedure or a commercial glycan detection kit, no saccharide was detected by carbohydrate analyses after acid hydrolysis or methanolysis. Identity of the amino acid sequence determined by analysis with that deduced from the gene sequence is further evidence of the absence of bound carbohydrate. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 6 |
| Volume Number | 92 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1995-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Amino Acid Oxidoreductases Chemistry Bacterial Proteins Clostridium Enzymology Multienzyme Complexes Isolation & Purification Amino Acid Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Mass Spectrometry Molecular Sequence Data Molecular Weight Peptide Fragments Selenium Metabolism Selenoproteins Serine Endopeptidases Trypsin Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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