NDLI: Selenophosphate synthetase: detection in extracts of rat tissues by immunoblot assay and partial purification of the enzyme from the archaean Methanococcus vannielii.

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Selenophosphate synthetase: detection in extracts of rat tissues by immunoblot assay and partial purification of the enzyme from the archaean Methanococcus vannielii.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, I. Y. Stadtman, T. C.
Description	Author Affiliation: Kim IY ( Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-0320, USA.);
Abstract	In Escherichia coli and Salmonella typhimurium it has been shown that selenophosphate serves as the selenium donor for the conversion of seryl-tRNA to selenocysteyl-tRNA and for the synthesis of 2-selenouridine, a modified nucleoside present in tRNAs. Although selenocysteyl-tRNA also is formed in eukaryotes and is used for the specific insertion of selenocysteine into proteins, the precise mechanism of its biosynthesis from seryl-tRNA in these systems is not known. Because selenophosphate is extremely oxygen labile and difficult to identify in biological systems, we used an immunological approach to detect the possible presence of selenophosphate synthetase in mammalian tissues. With antibodies elicited to E. coli selenophosphate synthetase the enzyme was detected in extracts of rat brain, liver, kidney, and lung by immunoblotting. Especially high levels were detected in Methanococcus vannielii, a member of the domain Archaea, and the enzyme was partially purified from this source. It seems likely that the use of selenophosphate as a selenium donor is widespread in biological systems.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	17
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Bacterial Proteins Isolation & Purification Brain Enzymology Drosophila Proteins Escherichia Coli Methanococcus Phosphotransferases Amino Acid Sequence Animals Antibodies Metabolism Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Genetics Genes, Bacterial Immunoblotting Kidney Liver Molecular Sequence Data Molecular Weight Salmonella Typhimurium Sequence Homology, Amino Acid Comparative Study Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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