NDLI: Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones.

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Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones.

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Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ball, L. A. Whelan, S. P. Barr, J. N. Wertz, G. T.
Description	Author Affiliation: Whelan SP ( Department of Microbiology, Medical School, University of Alabama at Birmingham 35294, USA.);
Abstract	Infectious vesicular stomatitis virus (VSV), the prototypic nonsegmented negative-strand RNA virus, was recovered from a full-length cDNA clone of the viral genome. Bacteriophage T7 RNA polymerase expressed from a recombinant vaccinia virus was used to drive the synthesis of a genome-length positive-sense transcript of VSV from a cDNA clone in baby hamster kidney cells that were simultaneously expressing the VSV nucleocapsid protein, phosphoprotein, and polymerase from separate plasmids. Up to 10(5) infectious virus particles were obtained from transfection of 10(6) cells, as determined by plaque assays. This virus was amplified on passage, neutralized by VSV-specific antiserum, and shown to possess specific nucleotide sequence markers characteristic of the cDNA. This achievement renders the biology of VSV fully accessible to genetic manipulation of the viral genome. In contrast to the success with positive-sense RNA, attempts to recover infectious virus from negative-sense T7 transcripts were uniformly unsuccessful, because T7 RNA polymerase terminated transcription at or near the VSV intergenic junctions.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	18
Volume Number	92
Language	English
Publisher	National Academy of Sciences
Publisher Date	1995-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	DNA, Complementary DNA, Viral Vesicular Stomatitis Indiana Virus Isolation & Purification Animals Antibodies, Viral Immunology Bacteriophage T7 Enzymology Cells, Cultured Cloning, Molecular Cricetinae DNA-Directed RNA Polymerases Metabolism Genetic Markers Neutralization Tests RNA, Viral Biosynthesis Genetics Pathogenicity Viral Proteins Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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