NDLI: An inhibitor of p34cdc2/cyclin B that regulates the G2/M transition in Xenopus extracts.

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An inhibitor of p34cdc2/cyclin B that regulates the G2/M transition in Xenopus extracts.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, T. H. Kirschner, M. W.
Description	Author Affiliation: Lee TH ( Harvard Medical School, Department of Cell Biology, Boston, MA 02115, USA.);
Abstract	The activity of maturation-promoting factor (MPF), a protein kinase complex composed of p34cdc2 and cyclin B, is undetectable during interphase but rises abruptly at the G2/M transition to induce mitosis. After the synthesis of cyclin B, the suppression of MPF activity before mitosis has been attributed to the phosphorylation of p34cdc2 on sites (threonine-14 and tyrosine-15) that inhibit its catalytic activity. We previously showed that the activity of the mitotic p34cdc2/cyclin B complex is rapidly suppressed when added to interphase Xenopus extracts that lack endogenous cyclin B. Here we show that a mutant of p34cdc2 that cannot be inhibited by phosphorylation (threonine-14-->alanine, tyrosine-15-->phenylalanine) is also susceptible to inactivation, demonstrating that inhibitory mechanisms independent of threonine-14 and tyrosine-15 phosphorylation must exist. We have partially characterized this inhibitory pathway as one involving a reversible binding inhibitor of p34cdc2/cyclin B that is tightly associated with cell membranes. Kinetic analysis suggests that this inhibitor, in conjunction with the kinases that mediate the inhibitory phosphorylations on p34cdc2, maintains the interphase state in Xenopus; it may play an important role in the exact timing of the G2/M transition.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	1
Volume Number	93
Language	English
Publisher	National Academy of Sciences
Publisher Date	1996-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Cycle Cyclins Antagonists & Inhibitors Animals Cell Membrane Physiology Cell-Free System Interphase Membrane Proteins Chemistry Mitosis Molecular Weight Oocytes Phosphothreonine Phosphotyrosine Rabbits Xenopus Laevis Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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