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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Imafuku, I. Okazawa, H. Kanazawa, I. Mouradian, M. M. Minowa, M. T. Hamada, H. |
| Description | Author Affiliation: Okazawa H ( Department of Neurology, Faculty of Medicine, University of Tokyo, Japan.); |
| Abstract | Brn-4 is a member of the POU transcription factor family and is expressed in the central nervous system. In this study, we addressed whether Brn-4 regulates expression of the D1A dopamine receptor gene. We found a functional Brn-4 responsive element in the intron of this gene by means of cotransfection chloramphenical acetyltransferase assays. This region contains two consensus sequences for binding of POU factors. Gel mobility-shift assays using glutathione S-transferase-Brn-4 fusion protein indicated that Brn-4 binds to these sequences. Both these sites are essential for transactivation by Brn-4 because deletion of either significantly reduced this enhancer activity. In situ hybridization revealed colocalization of Brn-4 and D1A mRNAs at the level of a single neuron in the rat striatum where this dopamine receptor is most abundantly expressed. Gel mobility-supershift assay using rat striatal nuclear extract and Brn-4 antibody confirmed the presence of Brn-4 in this brain region and its ability to bind to its consensus sequences in the D1A gene. These data suggest a functional role for Brn-4 in the expression of the D1A dopamine receptor gene both in vitro and in vivo. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 21 |
| Volume Number | 93 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1996-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Receptors, Dopamine D1 Biosynthesis Transcription Factors Metabolism Transcription, Genetic Animals Chloramphenicol O-Acetyltransferase Cytomegalovirus DNA Primers Gene Expression Regulation Genes, Reporter Genetic Vectors Glutathione Transferase Oligonucleotide Probes Polymerase Chain Reaction Recombinant Fusion Proteins Transfection Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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