NDLI: The Dr1/DRAP1 heterodimer is a global repressor of transcription in vivo.

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The Dr1/DRAP1 heterodimer is a global repressor of transcription in vivo.

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The Dr1/DRAP1 heterodimer is a global repressor of transcription in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hampsey, M. Kim, S. Na, J. G. Reinberg, D.
Description	Author Affiliation: Kim S ( Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635, USA.);
Abstract	A general repressor extensively studied in vitro is the human Dr1/DRAP1 heterodimeric complex. To elucidate the function of Dr1 and DRAP1 in vivo, the yeast Saccharomyces cerevisiae Dr1/DRAP1 repressor complex was identified. The repressor complex is encoded by two essential genes, designated YDR1 and BUR6. The inviability associated with deletion of the yeast genes can be overcome by expressing the human genes. However, the human corepressor DRAP1 functions in yeast only when human Dr1 is coexpressed. The yDr1/Bur6 complex represses transcription in vitro in a reconstituted RNA polymerase II transcription system. Repression of transcription could be overcome by increasing the concentration of TATA-element binding protein (TBP). Consistent with the in vitro results, overexpression of YDR1 in vivo resulted in decreased mRNA accumulation. Furthermore, YDR1 overexpression impaired cell growth, an effect that could be rescued by overexpression of TBP. In agreement with our previous studies in vitro, we found that overexpression of Dr1 in vivo also affected the accumulation of RNA polymerase III transcripts, but not of RNA polymerase I transcripts. Our results demonstrate that Dr1 functions as a repressor of transcription in vivo and, moreover, directly targets TBP, a global regulator of transcription.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	94
Language	English
Publisher	National Academy of Sciences
Publisher Date	1997-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	ATP-Binding Cassette Transporters Physiology Fungal Proteins Phosphoproteins Genetics Repressor Proteins Saccharomyces Cerevisiae Proteins Saccharomyces Cerevisiae Transcription Factors Metabolism Amino Acid Sequence DNA-Binding Proteins Gene Expression Regulation, Fungal Genes, Fungal Genetic Complementation Test Molecular Sequence Data RNA, Fungal Biosynthesis RNA, Messenger Growth & Development Sequence Homology, Amino Acid Species Specificity TATA-Box Binding Protein Transcription, Genetic Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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