NDLI: Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 2000 Volume: 97 Issue: 26

Year: 2000 Volume: 97 Issue: 25

Year: 2000 Volume: 97 Issue: 24

Year: 2000 Volume: 97 Issue: 23

Year: 2000 Volume: 97 Issue: 22

Year: 2000 Volume: 97 Issue: 21

Year: 2000 Volume: 97 Issue: 20

Year: 2000 Volume: 97 Issue: 19

Year: 2000 Volume: 97 Issue: 18

Year: 2000 Volume: 97 Issue: 17

Year: 2000 Volume: 97 Issue: 16

The central cytoplasmic loop of the major facilitator superfamily of transport proteins governs efficient membrane insertion.

Artificial ecosystem selection.

Bacterial-type DNA holliday junction resolvases in eukaryotic viruses.

In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse.

Inhibition of growth and metastatic progression of pancreatic carcinoma in hamster after somatostatin receptor subtype 2 (sst2) gene expression and administration of cytotoxic somatostatin analog AN-238.

The Arabidopsis dnd1 “defense, no death” gene encodes a mutated cyclic nucleotide-gated ion channel

Transcription-coupled repair in yeast is independent from ubiquitylation of RNA pol II: implications for Cockayne's syndrome.

The spliceosomal snRNP core complex of Trypanosoma brucei: cloning and functional analysis reveals seven Sm protein constituents.

In vitro and in vivo evidence for orphan nuclear receptor RORalpha function in bone metabolism.

High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells.

New roles for cis-jasmone as an insect semiochemical and in plant defense.

Myotubularin, a protein tyrosine phosphatase mutated in myotubular myopathy, dephosphorylates the lipid second messenger, phosphatidylinositol 3-phosphate

The role of dynamic palmitoylation in $Ca^{2+}$ channel inactivation

The human GRAF gene is fused to MLL in a unique t(5;11)(q31;q23) and both alleles are disrupted in three cases of myelodysplastic syndrome/acute myeloid leukemia with a deletion 5q.

A systematic exploration of the influence of the protein stability on amyloid fibril formation in vitro

The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).

Kinetic and thermodynamic control of protein adsorption.

Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex.

Subnucleus-specific loss of neurons in medial thalamus of schizophrenics.

The pleiotropic response regulator DegU functions as a priming protein in competence development in Bacillus subtilis.

Down-regulation of p21WAF1/CIP1 or p27Kip1 abrogates antiestrogen-mediated cell cycle arrest in human breast cancer cells.

Molecular basis of protein kinase C-induced activation of ATP-sensitive potassium channels.

Snapshots of usher-mediated protein secretion and ordered pilus assembly.

Trans catalysis in Tn5 transposition.

Specific binding sites for alcohols and anesthetics on ligand-gated ion channels.

A dual role for the kinase-like domain of the tyrosine kinase Tyk2 in interferon-α signaling

A chromatin insulator protects retrovirus vectors from chromosomal position effects

Coreceptor restriction within the HLA-DQ locus for Epstein-Barr virus infection.

B cell maturation protein is a receptor for the tumor necrosis factor family member TALL-1.

Inactivation of wild-type p53 tumor suppressor by electrophilic prostaglandins.

Thermodynamic characterization of the interaction between TRAF2 and tumor necrosis factor receptor peptides by isothermal titration calorimetry.

Recognition of the 300-kDa mannose 6-phosphate receptor cytoplasmic domain by 47-kDa tail-interacting protein.

Rapid mapping of protein functional epitopes by combinatorial alanine scanning.

In vivo immunological function of mast cells derived from embryonic stem cells: an approach for the rapid analysis of even embryonic lethal mutations in adult mice in vivo.

The development of fatal myocarditis and polymyositis in mice heterozygous for IFN-gamma and lacking the SOCS-1 gene.

Lipophosphoglycan is a virulence factor distinct from related glycoconjugates in the protozoan parasite Leishmania major.

Hormone binding induces rapid proteasome-mediated degradation of thyroid hormone receptors.

Engineering blood meal-activated systemic immunity in the yellow fever mosquito, Aedes aegypti.

Speeding molecular recognition by using the folding funnel: the fly-casting mechanism.

Modulation of CTP:phosphocholine cytidylyltransferase by membrane curvature elastic stress.

The cysteine desulfurase, IscS, has a major role in in vivo Fe-S cluster formation in Escherichia coli.

Learning about pain: the neural substrate of the prediction error for aversive events.

Protein-interaction modules that organize nuclear function: FF domains of CA150 bind the phosphoCTD of RNA polymerase II.

A new role for a SNARE protein as a regulator of the Ypt7/Rab-dependent stage of docking.

Crystal structure of Escherichia coli CyaY protein reveals a previously unidentified fold for the evolutionarily conserved frataxin family.

Comparing sequenced segments of the tomato and Arabidopsis genomes: large-scale duplication followed by selective gene loss creates a network of synteny.

Selective inhibition of HIV replication in primary macrophages but not T lymphocytes by macrophage-derived chemokine.

Neuroprotective potential of a viral vector system induced by a neurological insult.

Shattering the diffraction limit of light: a revolution in fluorescence microscopy?

B cells in autoimmunity.

Pathogens and hosts: the dance is the same, the couples are different.

Striking a balance: modulation of the actin cytoskeleton by Salmonella.

Structure and function of pectic enzymes: virulence factors of plant pathogens.

Pseudomonas syringae Hrp type III secretion system and effector proteins.

Molecular and cell biology aspects of plague.

A framework for interpreting the leucine-rich repeats of the Listeria internalins.

Acyl-homoserine lactone quorum sensing in gram-negative bacteria: a signaling mechanism involved in associations with higher organisms.

Phenotypic variation and intracellular parasitism by histoplasma Capsulatum.

Exploitation of host cells by enteropathogenic Escherichia coli.

Genetic complexity of pathogen perception by plants: the example of Rcr3, a tomato gene required specifically by Cf-2.

Plants and animals share functionally common bacterial virulence factors.

Role of the cystic fibrosis transmembrane conductance regulator in innate immunity to Pseudomonas aeruginosa infections.

Bad bugs and beleaguered bladders: interplay between uropathogenic Escherichia coli and innate host defenses.

AvrPto-dependent Pto-interacting proteins and AvrPto-interacting proteins in tomato.

Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens.

Nitric oxide and salicylic acid signaling in plant defense.

The role of antimicrobial peptides in animal defenses.

Suramin inhibits initiation of defense signaling by systemin, chitosan, and a beta-glucan elicitor in suspension-cultured Lycopersicon peruvianum cells.

Toward elimination of discrepancies between theory and experiment: The rate constant of the atmospheric conversion of $SO_{3}$ to $H_{2}SO_{4}$

Information theory, atoms in molecules, and molecular similarity.

The N terminus of microsomal delta 9 stearoyl-CoA desaturase contains the sequence determinant for its rapid degradation.

Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP.

Unfolding and internalization of proteins by the ATP-dependent proteases ClpXP and ClpAP.

Mouse Sebox homeobox gene expression in skin, brain, oocytes, and two-cell embryos.

Hydrolytic editing by a class II aminoacyl-tRNA synthetase.

Ribozyme-mediated inhibition of HIV 1 suggests nucleolar trafficking of HIV-1 RNA.

The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex.

Accurate and rapid docking of protein-protein complexes on the basis of intermolecular nuclear overhauser enhancement data and dipolar couplings by rigid body minimization.

Energetics of the HIV gp120-CD4 binding reaction.

The NF-kappa B signal transduction pathway in aortic endothelial cells is primed for activation in regions predisposed to atherosclerotic lesion formation.

The junctional multidomain protein AF-6 is a binding partner of the Rap1A GTPase and associates with the actin cytoskeletal regulator profilin.

Neisseria meningitidis porin PorB interacts with mitochondria and protects cells from apoptosis.

Paxillin alpha and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation.

Expression of the gene encoding the proapoptotic Nip3 protein is induced by hypoxia.

Gastrulation defective is a serine protease involved in activating the receptor toll to polarize the Drosophila embryo.

Spatial control of cell differentiation in Myxococcus xanthus.

Placental expression of the nonclassical MHC class I molecule Mamu-AG at implantation in the rhesus monkey.

Evolutionary consequences of changes in species' geographical distributions driven by Milankovitch climate oscillations.

Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray.

3' poly(A) is dispensable for translation.

Prediction, identification, and artificial selection of DNA rearrangements in Rhizobium: toward a natural genomic design.

T cell homeostasis in patients with rheumatoid arthritis.

Conditional up-regulation of MHC class I in skeletal muscle leads to self-sustaining autoimmune myositis and myositis-specific autoantibodies.

Intratumoral injection of adenoviral vectors encoding tumor-targeted immunoconjugates for cancer immunotherapy.

Positron emission tomography provides molecular imaging of biological processes.

Cloning and expression of a novel human antibody-antigen pair associated with Felty's syndrome.

Pseudorabies virus expressing enhanced green fluorescent protein: A tool for in vitro electrophysiological analysis of transsynaptically labeled neurons in identified central nervous system circuits.

Spinophilin regulates the formation and function of dendritic spines.

Presenilin complexes with the C-terminal fragments of amyloid precursor protein at the sites of amyloid β-protein generation

Role of C-terminal domains of the G protein beta subunit in the activation of effectors.

Interleukin-6 is an essential, corticotropin-releasing hormone-independent stimulator of the adrenal axis during immune system activation.

Etiology of interepidemic periods of mosquito-borne disease.

A dynamic model of social network formation.

Year: 2000 Volume: 97 Issue: 15

Year: 2000 Volume: 97 Issue: 14

Year: 2000 Volume: 97 Issue: 13

Year: 2000 Volume: 97 Issue: 12

Year: 2000 Volume: 97 Issue: 11

Year: 2000 Volume: 97 Issue: 10

Year: 2000 Volume: 97 Issue: 9

Year: 2000 Volume: 97 Issue: 8

Year: 2000 Volume: 97 Issue: 7

Year: 2000 Volume: 97 Issue: 6

Year: 2000 Volume: 97 Issue: 5

Year: 2000 Volume: 97 Issue: 4

Year: 2000 Volume: 97 Issue: 3

Year: 2000 Volume: 97 Issue: 2

Year: 2000 Volume: 97 Issue: 1

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Singh, S. K. Maurizi, M. R. Hoskins, J. R. Wickner, S.
Description	Author Affiliation: Hoskins JR ( Laboratory of Molecular Biology and Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.);
Abstract	ClpA, a bacterial member of the Clp/Hsp100 chaperone family, is an ATP-dependent molecular chaperone and the regulatory component of the ATP-dependent ClpAP protease. To study the mechanism of binding and unfolding of proteins by ClpA and translocation to ClpP, we used as a model substrate a fusion protein that joined the ClpA recognition signal from RepA to green fluorescent protein (GFP). ClpAP degrades the fusion protein in vivo and in vitro. The substrate binds specifically to ClpA in a reaction requiring ATP binding but not hydrolysis. Binding alone is not sufficient to destabilize the native structure of the GFP portion of the fusion protein. Upon ATP hydrolysis the GFP fusion protein is unfolded, and the unfolded intermediate can be sequestered by ClpA if a nonhydrolyzable analog is added to displace ATP. ATP is required for release. We found that although ClpA is unable to recognize native proteins lacking recognition signals, including GFP and rhodanese, it interacts with those same proteins when they are unfolded. Unfolded GFP is held in a nonnative conformation while associated with ClpA and its release requires ATP hydrolysis. Degradation of unfolded untagged proteins by ClpAP requires ATP even though the initial ATP-dependent unfolding reaction is bypassed. These results suggest that there are two ATP-requiring steps: an initial protein unfolding step followed by translocation of the unfolded protein to ClpP or in some cases release from the complex.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	16
Volume Number	97
Language	English
Publisher	National Academy of Sciences
Publisher Date	2000-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Adenosine Triphosphatases Metabolism Serine Endopeptidases Adenosine Triphosphate DNA Primers Endopeptidase Clp Green Fluorescent Proteins Hydrolysis Luminescent Proteins Chemistry Protein Binding Protein Conformation Protein Denaturation Substrate Specificity Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in