NDLI: Direct visualization at the single-cell level of electrically mediated gene delivery.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2002 Volume: 99 Issue: 26

Year: 2002 Volume: 99 Issue: 25

Year: 2002 Volume: 99 Issue: 24

Year: 2002 Volume: 99 Issue: 23

Year: 2002 Volume: 99 Issue: 22

Year: 2002 Volume: 99 Issue: 21

Year: 2002 Volume: 99 Issue: 20

Year: 2002 Volume: 99 Issue: 19

Year: 2002 Volume: 99 Issue: 18

Year: 2002 Volume: 99 Issue: 17

Year: 2002 Volume: 99 Issue: 16

Year: 2002 Volume: 99 Issue: 15

Year: 2002 Volume: 99 Issue: 14

Year: 2002 Volume: 99 Issue: 13

Year: 2002 Volume: 99 Issue: 12

Year: 2002 Volume: 99 Issue: 11

Year: 2002 Volume: 99 Issue: 10

Year: 2002 Volume: 99 Issue: 9

Year: 2002 Volume: 99 Issue: 8

Year: 2002 Volume: 99 Issue: 7

Year: 2002 Volume: 99 Issue: 6

Year: 2002 Volume: 99 Issue: 5

Year: 2002 Volume: 99 Issue: 4

Year: 2002 Volume: 99 Issue: Suppl 4

Year: 2002 Volume: 99 Issue: 3

Conformational landscape of cytochrome c folding studied by microsecond-resolved small-angle x-ray scattering.

Visual arrestins in olfactory pathways of Drosophila and the malaria vector mosquito Anopheles gambiae.

Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria.

Antigen-presenting dendritic cells provide the reducing extracellular microenvironment required for T lymphocyte activation.

Functional p53 chimeras containing the Epstein-Barr virus Gly-Ala repeat are protected from Mdm2- and HPV-E6-induced proteolysis.

Cytosolic malate dehydrogenase confers selectivity of the nucleic acid-conducting channel.

The evolution and development of cranial form in Homosapiens.

Microbat paraphyly and the convergent evolution of a key innovation in Old World rhinolophoid microbats.

Subnuclear distribution of topoisomerase I is linked to ongoing transcription and p53 status.

Potent antiarthritic properties of a glucocorticoid derivative, NCX-1015, in an experimental model of arthritis.

IL-6 transgenic mouse model for extraosseous plasmacytoma.

Human macrophage activation programs induced by bacterial pathogens.

Type I collagen is thermally unstable at body temperature.

The intramembrane cleavage site of the amyloid precursor protein depends on the length of its transmembrane domain

Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes.

Protein topology and stability define the space of allowed sequences.

Photoactivation of the flavin cofactor in Xenopus laevis (6 - 4) photolyase: observation of a transient tyrosyl radical by time-resolved electron paramagnetic resonance.

HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.

C2A activates a cryptic $Ca^{2+}-triggered$ membrane penetration activity within the C2B domain of synaptotagmin I

Testosterone prevents the heat shock-induced overactivation of glycogen synthase kinase-3 beta but not of cyclin-dependent kinase 5 and c-Jun NH2-terminal kinase and concomitantly abolishes hyperphosphorylation of tau: implications for Alzheimer's disease.

Cerebellar cortical inhibition and classical eyeblink conditioning.

Guiding the study of brain dynamics by using first-person data: synchrony patterns correlate with ongoing conscious states during a simple visual task.

The origin of the eukaryotic cell: a genomic investigation.

Adenovirus-directed expression of Q227L-G alpha(s) inhibits growth of established tumors of later-stage human breast cancer cells in athymic mice.

Recurrent evolution of DNA-binding motifs in the Drosophila centromeric histone.

Role of backbone solvation in determining thermodynamic beta propensities of the amino acids.

Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm.

Analysis of Cdc6 function in the assembly of mammalian prereplication complexes.

Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution.

The thioesterase domain from a nonribosomal peptide synthetase as a cyclization catalyst for integrin binding peptides.

Self-generated DNA termini relax the specificity of SgrAI restriction endonuclease.

Anthropogenic modification of New England salt marsh landscapes.

A cell number-counting factor regulates the cytoskeleton and cell motility in Dictyostelium.

Determinants of cluster distribution in the molecular epidemiology of tuberculosis.

Nitrogen assimilation and growth of wheat under elevated carbon dioxide

The mechanism of catalysis of the chorismate to prephenate reaction by the Escherichia coli mutase enzyme.

Genetic analysis of Pten and Ink4a/Arf interactions in the suppression of tumorigenesis in mice.

gamma -Glutamyl carboxylation: An extracellular posttranslational modification that antedates the divergence of molluscs, arthropods, and chordates.

Spatial frequency and orientation tuning dynamics in area V1.

Efficacy and age-related effects of nitric oxide-releasing aspirin on experimental restenosis.

Spirochete and protist symbionts of a termite (Mastotermes electrodominicus) in Miocene amber.

A direct role of SRY and SOX proteins in pre-mRNA splicing.

Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice.

Direct visualization at the single-cell level of electrically mediated gene delivery.

Cultured rat hippocampal neural progenitors generate spontaneously active neural networks.

Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system.

Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP.

The fibrinolytic receptor for urokinase activates the G protein-coupled chemotactic receptor FPRL1/LXA4R.

Conformational Abs recognizing a generic amyloid fibril epitope.

mtDNA from fossils reveals a radiation of Hawaiian geese recently derived from the Canada goose (Brantacanadensis).

Evidence of a mate-finding cue in the hermaphrodite nematode Caenorhabditis elegans.

Generation of an activating $Zn^{2+}$ switch in the dopamine transporter: Mutation of an intracellular tyrosine constitutively alters the conformational equilibrium of the transport cycle

Extracellular ubiquitination and proteasome-mediated degradation of the ascidian sperm receptor.

Casein kinase I phosphorylates and destabilizes the beta-catenin degradation complex.

Molecular phylogenetic evidence for the independent evolutionary origin of an arthropod compound eye.

Flexibility and packing in proteins.

Focal loss of the glutamate transporter EAAT2 in a transgenic rat model of SOD1 mutant-mediated amyotrophic lateral sclerosis (ALS).

Structural basis for the conformational adaptability of apolipophorin III, a helix-bundle exchangeable apolipoprotein.

Cho, a second endonuclease involved in Escherichia coli nucleotide excision repair.

Stable suppression of gene expression by RNAi in mammalian cells.

Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.

Selective up-regulation of dopamine D1 receptors in dendritic spines by NMDA receptor activation.

The unexpected landscape of in vivo somatic mutation in a human epithelial cell lineage.

Type 3 ryanodine receptors of skeletal muscle are segregated in a parajunctional position

Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein.

Oscillatory brain states and learning: Impact of hippocampal theta-contingent training.

Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity.

The enthalpy of the alanine peptide helix measured by isothermal titration calorimetry using metal-binding to induce helix formation.

Probing protein conformational changes in living cells by using designer binding proteins: application to the estrogen receptor.

Redundancy, antiredundancy, and the robustness of genomes.

Beta-amyloid precursor protein transgenic mice that harbor diffuse A beta deposits but do not form plaques show increased ischemic vulnerability: role of inflammation.

Combinatorial computational method gives new picomolar ligands for a known enzyme.

Neurotrophic factor intervention restores auditory function in deafened animals.

Importance of epidermal growth factor receptor signaling in establishment of adenomas and maintenance of carcinomas during intestinal tumorigenesis.

Dendritic spines elongate after stimulation of group 1 metabotropic glutamate receptors in cultured hippocampal neurons.

Dynamic association of transcriptional activation domains and regulatory regions in Saccharomyces cerevisiae heat shock factor.

Anatomical and physiological evidence for involvement of tuberoinfundibular peptide of 39 residues in nociception.

Comparative genomic analysis of Vibrio cholerae: Genes that correlate with cholera endemic and pandemic disease

Uncoating ATPase Hsc70 is recruited by invariant chain and controls the size of endocytic compartments.

Dissociation in human prefrontal cortex of affective influences on working memory-related activity.

Cytochrome c release from mitochondria proceeds by a two-step process.

Coherent infrared emission from myoglobin crystals: an electric field measurement.

Human Elongator facilitates RNA polymerase II transcription through chromatin.

The regulatory role for the ERCC3 helicase of general transcription factor TFIIH during promoter escape in transcriptional activation.

Leaf-to-shoot apex movement of symplastic tracer is restricted coincident with flowering in Arabidopsis.

Viewing protein folding from many perspectives.

Unstable molecules form stable tissues.

The methylator meets the terminator.

Antigen-presenting cells control T cell proliferation by regulating amino acid availability.

Location alters tRNA identity: Trypanosoma brucei's cytosolic elongator tRNAMet is both the initiator and elongator in mitochondria.

Dissecting the molecular mechanisms of olfaction in a malaria-vector mosquito.

Calcium signaling: A tale for all seasons

Stochastic and mesoscopic models for tropical convection.

The calculation of transport properties in quantum liquids using the maximum entropy numerical analytic continuation method: application to liquid para-hydrogen.

Product dependence and bifunctionality compromise the ultrasensitivity of signal transduction cascades.

Discrimination against purine-pyrimidine mispairs in the polymerase active site of DNA polymerase I: a structural explanation.

Structural diversity of heparan sulfate binding domains in chemokines.

Translocation of a functional protein by a voltage-dependent ion channel.

Stable 'zeta' peptides that act as potent antagonists of the high-affinity IgE receptor.

Muscle-derived hematopoietic stem cells are hematopoietic in origin.

Projecting the future of the U.S. carbon sink.

The analysis of 100 genes supports the grouping of three highly divergent amoebae: Dictyostelium, Entamoeba, and Mastigamoeba.

Complementary whole-genome technologies reveal the cellular response to proteasome inhibition by PS-341.

The murine CCR3 receptor regulates both the role of eosinophils and mast cells in allergen-induced airway inflammation and hyperresponsiveness.

Cooperation of multiple signaling pathways in CD40-regulated gene expression in B lymphocytes.

Targeting the prostate for destruction through a vascular address.

The Brucella suis virB operon is induced intracellularly in macrophages.

The murMN operon: a functional link between antibiotic resistance and antibiotic tolerance in Streptococcuspneumoniae.

DNA microarray analysis of differential gene expression in Borrelia burgdorferi, the Lyme disease spirochete.

The capsular polysaccharide of Enterococcus faecalis and its relationship to other polysaccharides in the cell wall.

The SNARE protein SNAP-25 is linked to fast calcium triggering of exocytosis

Circulating relaxin acts on subfornical organ neurons to stimulate water drinking in the rat.

A gene encoding a protein modified by the phytohormone indoleacetic acid.

Enhanced ADP-glucose pyrophosphorylase activity in wheat endosperm increases seed yield.

Global organization of the Wordnet lexicon.

Year: 2002 Volume: 99 Issue: Suppl 3

Year: 2002 Volume: 99 Issue: 2

Year: 2002 Volume: 99 Issue: Suppl 2

Year: 2002 Volume: 99 Issue: 1

Year: 2002 Volume: 99 Issue: Suppl 1

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Direct visualization at the single-cell level of electrically mediated gene delivery.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Golzio, Muriel Rols, Marie-pierre Teissie, Justin
Description	Author Affiliation: Golzio M ( Institut de Pharmacologie et de Biologie Structurale Centre National de la Recherche Scientifique/Unité Mixte de Recherche-5089, 205, Route de Narbonne, 31077 Toulouse Cedex, France.);
Abstract	Electropermeabilization is one of the nonviral methods successfully used to transfer genes into living cells in vitro and in vivo. Although this approach shows promise in the field of gene therapy, very little is known about the basic processes supporting DNA transfer. The present investigation studies this process at the single-cell level by using digitized fluorescence microscopy. Permeabilization is a prerequisite for gene transfer. Its assay by propidium-iodide (PI) penetration shows that it occurs at the sides of the cell membrane facing the two electrodes, whereas fluorescently labeled plasmids only interact with the electropermeabilized side of the cell facing the cathode. The plasmid interaction with the electropermeabilized part of the cell surface results in the formation of localized aggregates. These membrane-associated spots are formed only when pulses with a longer duration than a critical value are applied. These complexes are formed within 1 s after the pulses and cannot be destroyed by pulses of reversed polarities. They remain at the membrane level up to 10 min after pulsing. Although freely accessible to DNA dye (TOTO-1) 1 min after the pulses, they are fully protected when the addition takes place 10 min after. They diffuse in the cytoplasm 30 min after pulses and are present around the nucleus 24 h later.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Electroporation Luminescent Proteins Genetics Transfection Animals Biological Transport CHO Cells Cell Membrane Physiology Cell Membrane Permeability Cricetinae DNA Electrophysiology Fluorescent Dyes Green Fluorescent Proteins Metabolism Plasmids Recombinant Proteins Thiazoles Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in