NDLI: The cAMP-specific phosphodiesterase TbPDE2C is an essential enzyme in bloodstream form Trypanosoma brucei.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2002 Volume: 99 Issue: 26

Year: 2002 Volume: 99 Issue: 25

Year: 2002 Volume: 99 Issue: 24

Year: 2002 Volume: 99 Issue: 23

Year: 2002 Volume: 99 Issue: 22

Year: 2002 Volume: 99 Issue: 21

Year: 2002 Volume: 99 Issue: 20

Year: 2002 Volume: 99 Issue: 19

Year: 2002 Volume: 99 Issue: 18

Year: 2002 Volume: 99 Issue: 17

Year: 2002 Volume: 99 Issue: 16

Year: 2002 Volume: 99 Issue: 15

Year: 2002 Volume: 99 Issue: 14

Year: 2002 Volume: 99 Issue: 13

Year: 2002 Volume: 99 Issue: 12

Year: 2002 Volume: 99 Issue: 11

Year: 2002 Volume: 99 Issue: 10

Year: 2002 Volume: 99 Issue: 9

Year: 2002 Volume: 99 Issue: 8

Year: 2002 Volume: 99 Issue: 7

A mitochondrial-like aconitase in the bacterium Bacteroides fragilis: implications for the evolution of the mitochondrial Krebs cycle.

Social intelligence, innovation, and enhanced brain size in primates.

Large-scale analysis of the human and mouse transcriptomes.

Directed evolution of the site specificity of Cre recombinase

Atmospheric carbon dioxide levels for the last 500 million years

Inhibition of Xenopus oocyte meiotic maturation by catalytically inactive protein kinase A.

Nitrogen availability alters the expression of carnivory in the northern pitcher plant, Sarracenia purpurea.

Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: a tool for efficient genetic engineering of mammalian genomes.

Conformational flexibility of bacterial RNA polymerase.

Emission lines of [K V] in the optical spectra of gaseous nebulae.

In vivo NMR microscopy allows short-term serial assessment of multiple skeletal implications of corticosteroid exposure.

Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution.

Regulation of PKR and IRF-1 during hepatitis C virus RNA replication.

Adenosine deaminase-related growth factors stimulate cell proliferation in Drosophila by depleting extracellular adenosine.

HNF-1alpha G319S, a transactivation-deficient mutant, is associated with altered dynamics of diabetes onset in an Oji-Cree community.

The Caenorhabditis elegans mucolipin-like gene cup-5 is essential for viability and regulates lysosomes in multiple cell types.

Extreme genome reduction in Buchnera spp.: toward the minimal genome needed for symbiotic life.

Computational mapping identifies the binding sites of organic solvents on proteins.

HLTF gene silencing in human colon cancer.

Analysis of cell-cycle-specific gene expression in human cells as determined by microarrays and double-thymidine block synchronization.

Using RNA interference to identify genes required for RNA interference.

Mammalian germ-line transgenesis by transposition

Origin of sphinx, a young chimeric RNA gene in Drosophila melanogaster.

Curing metastatic testicular cancer.

Transcription-coupled and DNA damage-dependent ubiquitination of RNA polymerase II in vitro.

Actions of PP2A on the MAP kinase pathway and apoptosis are mediated by distinct regulatory subunits.

$PIP_{3}$ inhibition of RGS protein and its reversal by $Ca^{2+}/calmodulin$ mediate voltage-dependent control of the G protein cycle in a cardiac $K^{+}$ channel

Molecular phenotyping for analyzing subtle genetic effects in mice: application to an angiotensinogen gene titration.

Bartonella-associated endothelial proliferation depends on inhibition of apoptosis.

Algal blooms reduce the uptake of toxic methylmercury in freshwater food webs

Oligomerization and activation of caspase-9, induced by Apaf-1 CARD.

Endoplasmic reticulum localization of the low density lipoprotein receptor mediates presecretory degradation of apolipoprotein B.

Gramicidin A channels switch between stretch activation and stretch inactivation depending on bilayer thickness.

Impaired cardiac hypertrophic response in Calcineurin Abeta -deficient mice.

Dissociation of peripheral T cell responses from thymocyte negative selection by weak agonists supports a spare receptor model of T cell activation.

Whole-genome disassembly.

On the sequencing and assembly of the human genome.

Molecules, muscles, and machines: universal performance characteristics of motors.

A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli.

Paranodal junction formation and spermatogenesis require sulfoglycolipids.

Endothelial cells derived from human embryonic stem cells.

Rate heterogeneity among lineages of tracheophytes: integration of molecular and fossil data and evidence for molecular living fossils.

Examination of hypotheses for the Permo–Triassic boundary extinction by carbon cycle modeling

Vav3 is regulated during the cell cycle and effects cell division.

In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis.

Reversal of motor impairments in parkinsonian rats by continuous intrastriatal delivery of L-dopa using rAAV-mediated gene transfer.

Identification of a protein essential for a major pathway used by human cells to avoid UV- induced DNA damage.

Stimulation of bone formation and prevention of bone loss by prostaglandin E EP4 receptor activation.

Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks

Circadian 5-HT production regulated by adrenergic signaling.

Naive T cells proliferate strongly in neonatal mice in response to self-peptide/self-MHC complexes.

Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae.

A physiologic signaling role for the gamma -secretase-derived intracellular fragment of APP.

Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation.

Desensitization of mu-opioid receptor-evoked potassium currents: initiation at the receptor, expression at the effector.

Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles

Mutation of melanosome protein RAB38 in chocolate mice.

Lineage infidelity in myeloid cells with TCR gene rearrangement: a latent developmental potential of proT cells revealed by ectopic cytokine receptor signaling.

The procaspase-8 isoform, procaspase-8L, recruited to the BAP31 complex at the endoplasmic reticulum.

Leukotriene A4 hydrolase: selective abrogation of leukotriene B4 formation by mutation of aspartic acid 375.

Human receptors for sweet and umami taste.

Function of quaking in myelination: regulation of alternative splicing.

Elucidating the biosynthesis of 2-carboxyarabinitol 1-phosphate through reduced expression of chloroplastic fructose 1,6-bisphosphate phosphatase and radiotracer studies with $^{14}CO_{2}$

Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558 II.

Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2.

Chair-boat transitions in single polysaccharide molecules observed with force-ramp AFM.

MYC overexpression imposes a nonimmunogenic phenotype on Epstein-Barr virus-infected B cells.

DnaK dependence of mutant ethanol oxidoreductases evolved for aerobic function and protective role of the chaperone against protein oxidative damage in Escherichia coli

A role for survivin in chemoresistance of endothelial cells mediated by VEGF.

Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro.

Keratin binding to 14-3-3 proteins modulates keratin filaments and hepatocyte mitotic progression.

The homeobox gene BREVIPEDICELLUS is a key regulator of inflorescence architecture in Arabidopsis.

Cell membrane GM1 ganglioside is a functional coreceptor for fibroblast growth factor 2.

The accessory subunit of DNA polymerase gamma is essential for mitochondrial DNA maintenance and development in Drosophila melanogaster.

Genetic evidence for Shc requirement in TCR-induced c-Rel nuclear translocation and IL-2 expression.

Imaging the progression of Alzheimer pathology through the brain.

From bacteria to mitochondria: aconitase yields surprises.

What are big brains for?

Dynamics of self assembly of magnetized disks rotating at the liquid-air interface.

Phosphate backbone neutralization increases duplex DNA flexibility: a model for protein binding.

The Pythagorean Theorem: I. The finite case.

The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain.

A functional chaperone triad on the yeast ribosome.

Selenoprotein R is a zinc-containing stereo-specific methionine sulfoxide reductase

Diversity and evolution of the green fluorescent protein family.

Rapid compaction during RNA folding.

Fluorescence polarization of green fluorescence protein.

$Mg^{2+}-dependent$ conformational change of RNA studied by fluorescence correlation and FRET on immobilized single molecules

FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction.

The cAMP-specific phosphodiesterase TbPDE2C is an essential enzyme in bloodstream form Trypanosoma brucei.

Renewable and nonrenewable resources: amino acid turnover and allocation to reproduction in Lepidoptera.

Evolutionary conservation of chromosome territory arrangements in cell nuclei from higher primates.

Sexual selection driving diversification in jumping spiders.

Mitf and Tfe3, two members of the Mitf-Tfe family of bHLH-Zip transcription factors, have important but functionally redundant roles in osteoclast development.

The combined absence of NF-κB1 and c-Rel reveals that overlapping roles for these transcription factors in the B cell lineage are restricted to the activation and function of mature cells

Role of poly(ADP-ribosyl)ation in DNA-PKcs- independent V(D)J recombination.

Osteopontin deficiency protects joints against destruction in anti-type II collagen antibody-induced arthritis in mice.

Panhandle and reverse-panhandle PCR enable cloning of der(11) and der(other) genomic breakpoint junctions of MLL translocations and identify complex translocation of MLL, AF-4, and CDK6.

Severe iron deficiency anemia in transgenic mice expressing liver hepcidin.

Bradykinin antagonist dimer, CU201, inhibits the growth of human lung cancer cell lines by a “biased agonist” mechanism

Genes directly controlled by CtrA, a master regulator of the Caulobacter cell cycle.

Genetic control of quorum-sensing signal turnover in Agrobacterium tumefaciens.

The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.

Gene expression analysis in schizophrenia: reproducible up-regulation of several members of the apolipoprotein L family located in a high-susceptibility locus for schizophrenia on chromosome 22.

Mapping the evolution of regional atrophy in Alzheimer's disease: unbiased analysis of fluid-registered serial MRI.

Cloning and characterization of a cAMP-specific phosphodiesterase (TbPDE2B) from Trypanosoma brucei.

Structural requirement of bilin chromophore for the photosensory specificity of phytochromes A and B.

GCR1, the putative Arabidopsis G protein-coupled receptor gene is cell cycle-regulated, and its overexpression abolishes seed dormancy and shortens time to flowering.

Chaos in learning a simple two-person game.

Year: 2002 Volume: 99 Issue: 6

Year: 2002 Volume: 99 Issue: 5

Year: 2002 Volume: 99 Issue: 4

Year: 2002 Volume: 99 Issue: Suppl 4

Year: 2002 Volume: 99 Issue: 3

Year: 2002 Volume: 99 Issue: Suppl 3

Year: 2002 Volume: 99 Issue: 2

Year: 2002 Volume: 99 Issue: Suppl 2

Year: 2002 Volume: 99 Issue: 1

Year: 2002 Volume: 99 Issue: Suppl 1

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

The cAMP-specific phosphodiesterase TbPDE2C is an essential enzyme in bloodstream form Trypanosoma brucei.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Seebeck, Thomas Zoraghi, Roya
Description	Author Affiliation: Zoraghi R ( Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH-3012 Bern, Switzerland.);
Abstract	Chemotherapy of human sleeping sickness, a fatal disease caused by the protozoan parasite Trypanosoma brucei, is in a dismal state, and the identification and characterization of new drug targets is an urgent prerequisite for an improvement of the dramatic situation in the field. Over the last several years, inhibitors of cyclic nucleotide-specific phosphodiesterases have proven to be highly successful drug candidates for an assortment of clinical conditions. Their potential as antiparasitic drugs has not been explored so far. This study reports the characterization of a cAMP-specific phosphodiesterase from T. brucei, TbPDE2C. This enzyme is a class I phosphodiesterase, and it is a member of a small enzyme family in T. brucei, TbPDE2. Inhibitors of this enzyme block the proliferation of bloodstream form trypanosomes in culture. RNA interference experiments demonstrated that the TbPDE2 family, and in particular TbPDE2C, are essential for maintaining intracellular cAMP concentrations within a physiological range. Bloodstream form trypanosomes are exquisitely sensitive to elevated concentrations of intracellular cAMP, and a disruption of TbPDE2C function quickly leads to the disruption of nuclear and cellular cell division, and to cell death. TbPDE2C might represent a novel drug target for the development of new and effective trypanocidal drugs.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Parasitemia Parasitology Phosphoric Diester Hydrolases Physiology Trypanosoma Brucei Brucei Enzymology Amino Acid Sequence Animals Catalysis Cyclic AMP Cyclic Nucleotide Phosphodiesterases, Type 2 Molecular Sequence Data Chemistry Genetics RNA, Double-Stranded Pharmacology RNA, Messenger Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in