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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hohn, Thomas Stavolone, Livia Leclerc, Denis Villani, Maria Elena |
| Description | Author Affiliation: Stavolone L ( Friedrich Miescher Institute, P.O. Box 2543, CH-4002, Basel, Switzerland. l.stavolone@ba.ivv.cnr.it); |
| Abstract | The function of the virion-associated protein (VAP) of cauliflower mosaic virus (CaMV) has long been only poorly understood. VAP is associated with the virion but is dispensable for virus morphogenesis and replication. It mediates virus transmission by aphids through simultaneous interaction with both the aphid transmission factor and the virion. However, although insect transmission is not fundamental to CaMV survival, VAP is indispensable for spreading the virus infection within the host plant. We used a GST pull-down technique to demonstrate that VAP interacts with the viral movement protein through coiled-coil domains and surface plasmon resonance to measure the interaction kinetics. We mapped the movement protein coiled-coil to the C terminus of the protein and proved that it self-assembles as a trimer. Immunogold labeling/electron microscopy revealed that the VAP and viral movement protein colocalize on CaMV particles within plasmodesmata. These results highlight the multifunctional potential of the VAP protein conferred by its efficient coiled-coil interaction system and show a plant virus possessing a surface-exposed protein (VAP) mediating viral entry into host cells. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 17 |
| Volume Number | 102 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2005-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Caulimovirus Physiology Amino Acid Sequence Genetics Isolation & Purification Ultrastructure Chromatography, Gel Escherichia Coli Genes, Reporter Glutathione Transferase Molecular Sequence Data Open Reading Frames Plants Virology Surface Plasmon Resonance Viral Proteins Chemistry Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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