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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nijagal, Amar Ray, Pritha Kim, Young J. Shelly, Stephanie M. Gambhir, Sanjiv S. Witte, Owen N. Radu, Caius G. Guo, Shuling Shu, Chengyi J. |
| Description | Author Affiliation: Shu CJ ( Department of Microbiology, Immunology, and Molecular Genetics, Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, USA.); |
| Abstract | Current methodologies that monitor immune responses rely on invasive techniques that sample tissues at a given point in time. New technologies are needed to elucidate the temporal patterns of immune responses and the spatial distribution of immune cells on a whole-body scale. We describe a noninvasive, quantitative, and tomographic approach to visualize a primary anti-tumor immune response by using positron emission tomography (PET). Bone marrow chimeric mice were generated by engraftment of hematopoietic stem and progenitor cells transduced with a trifusion reporter gene encoding synthetic Renilla luciferase (hRluc), EGFP, and Herpes virus thymidine kinase (sr39TK). Mice were challenged with the Moloney murine sarcoma and leukemia virus complex (M-MSV/M-MuLV), and the induced immune response was monitored by using PET. Hematopoietic cells were visualized by using 9-[4-[(18)F]fluoro-3-(hydroxymethyl)butyl]guanine ([(18)F]FHBG), a radioactive substrate specific for the sr39TK PET reporter protein. Immune cell localization and expansion were seen at the tumor and draining lymph nodes (DLNs). 2-[(18)F]fluoro-2-deoxy-D-glucose ([(18)F]FDG), which is sequestered in metabolically active cells, was used to follow tumor growth and regression. Elevated glucose metabolism was also seen in activated lymphocytes in the DLNs by using the [(18)F]FDG probe. When M-MSV/M-MuLV-challenged mice were treated with the immunosuppressive drug dexamethasone, activation and expansion of immune cell populations in the DLNs could no longer be detected with PET imaging. The method we describe can be used to kinetically measure the induction and therapeutic modulations of cell-mediated immune responses. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 48 |
| Volume Number | 102 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2005-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Moloney Murine Leukemia Virus Immunology Moloney Murine Sarcoma Virus Retroviridae Infections Sarcoma, Experimental Tumor Virus Infections Animals Bone Marrow Transplantation Dexamethasone Therapeutic Use Flow Cytometry Fluorodeoxyglucose F18 Green Fluorescent Proteins Guanine Analogs & Derivatives Hematopoietic Stem Cells Immunohistochemistry Luciferases Lymph Nodes Mice Mice, Inbred C57BL Mice, SCID Positron-Emission Tomography Drug Therapy Thymidine Kinase Comparative Study Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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