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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | James, Chloe E. Lamont, Richard J. Tribble, Gena D. Mao, Song |
| Description | Author Affiliation: Tribble GD ( Department of Oral Biology, University of Florida School of Dentistry, Gainesville, FL 32610-0424, USA.); |
| Abstract | Haloacid dehalogenase (HAD) family phosphatases are widespread in prokaryotes and are generally involved in metabolic processes. Porphyromonas gingivalis, an invasive periodontal pathogen, secretes the HAD family phosphoserine phosphatase SerB653 when in contact with gingival epithelial cells. Here we characterize the structure and enzymatic activity of SerB653 and show that a SerB653 allelic replacement mutant of P. gingivalis is deficient in internalization and persistence in gingival epithelial cells. In contrast, mutation of a second HAD family serine phosphatase of P. gingivalis (SerB1170), or of a serine transporter, did not affect invasion. A pull-down assay identified GAPDH and heat-shock protein 90 as potential substrates for SerB653. Furthermore, exogenous phosphatase regulated microtubule dynamics in host cells. These data indicate that P. gingivalis has adapted a formerly metabolic enzyme to facilitate entry into host cells by modulating host cytoskeletal architecture. Our findings define a virulence-related role of a HAD family phosphatase and reveal an invasin of an important periodontal pathogen. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 29 |
| Volume Number | 103 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2006-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacterial Adhesion Hydrolases Classification Metabolism Phosphoproteins Phosphoric Monoester Hydrolases Porphyromonas Gingivalis Physiology Alleles Amino Acid Sequence Amino Acids Cell Line Enzyme Inhibitors Pharmacology Chemistry Genetics Hydrophobic And Hydrophilic Interactions Microtubules Molecular Sequence Data Mutation Phosphorylation Protein Binding Substrate Specificity Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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