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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Das, Biswadip Das, Satarupa Sherman, Fred |
| Description | Author Affiliation: Das B ( Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA.); |
| Abstract | We previously demonstrated that mRNAs retained in the nucleus of Saccharomyces cerevisiae are subjected to a degradation system-designated DRN (degradation of mRNA in the nucleus), that is diminished in cbc1-Delta or cbc2-Delta mutants lacking components of the cap-binding complex and in rrp6-Delta mutants lacking Rrp6p, a 3' to 5' nuclear exonuclease. Two mutants, lys2-187 and lys2-121, were uncovered by screening numerous lys2 mutants for suppression by cbc1-Delta and rrp6-Delta. Both mutants were identical and contained the two base changes, one of which formed a TGA nonsense codon. LYS2 mRNAs from the lys2-187 and related mutants were rapidly degraded, and the degradation was suppressed by cbc1-Delta and rrp6-Delta. The U1A-GFP imaging procedure was used to show that the lys2-187 mRNA was partially retained in the nucleus, explaining the susceptibility to DRN. The creation of several derivatives of lys2-187 by site-directed mutagenesis revealed that the in-frame TGA by itself was not responsible for the increased susceptibility to DRN. Thus, mRNAs susceptible to DRN can be formed by a 2-bp change. Furthermore, this 'retention signal' causing susceptibility to DRN is lost by altering one of the base pairs, establishing that mRNAs susceptible and unsusceptible to DRN can be attributed to a single nucleotide in the proper context. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 29 |
| Volume Number | 103 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2006-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Nucleus Genetics Metabolism Saccharomyces Cerevisiae Classification Drug Effects Cytoplasm DNA, Fungal Chemistry Exoribonucleases Exosome Multienzyme Ribonuclease Complex Gene Deletion Gene Expression Regulation, Fungal Molecular Sequence Data Mutation Nuclear Proteins Paromomycin Pharmacology RNA Cap-Binding Proteins RNA Stability RNA, Messenger Saccharomyces Cerevisiae Proteins Research Support, N.I.H., Extramural Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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